Abstract
Mineral-microbe interactions are widespread in a number of environmental processes such as mineral weathering, decomposition, and transformation. Both clay minerals and silicate-weathering bacteria are widely distributed in nature, and the latter contribute to weathering, diagenesis, and mineralization of major rock-forming minerals. The purpose of this study was to observe changes in the chemical composition and structure, especially the phase transformation, of smectite after processing by a silicate-weathering bacterium. The interaction between Bacillus mucilaginosus and bentonite was studied using custom culture media. Results from Inductively Coupled Plasma-Atomic Emission Spectrometry revealed that the bacterium promoted release of Si and Al from solid bentonite to solution. Concomitantly, the Ka nd Fe contents of the mineral increased as shown by X-ray photoelectron spectroscopy results. After interaction with the bacterium, the montmorillonite underwent a possible structure transformation to smectite, as indicated by the emergence of a new weak peak (d = 9.08 Å) shown by X-ray diffraction patterns. The mineralogical changes were also demonstrated by the decrease in the specific surface area of the mineral from 33.0 to 24.0 m2/g (these lower values for SSA of bentonite are related to the particle size of the smectite examined (120-160 mesh) and the weakened absorption bands in Al-O-H and Si-O-Si vibrations by Micro Fourier-transform infrared spectroscopy. The morphology changes in the bacteria observed by environmental scanning electron microscopy and atomic force microscopy revealed an obvious growth of the flagella in the presence of bentonite.
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References
Aleksandrov, V.G. (1953) Silicate Bacteria. Science Publishing House, Beijing.
Boyle, J.R. and Voigt, G.K. (1973) Biological weathering of silicate for tree nutrition and soil genesis. Plant and Soil, 38, 191–201.
Dong, H.L. (2010) Mineral-microbe interactions: a review. Frontiers of Earth Science in China, 4, 127–147.
Dong, H.L., Jaisi, D.P., Kim, J., and Zhang, G.X. (2009) Microbe-clay mineral interactions. American Mineralogist, 94, 1505–1519.
Ehrlich, H.L. (1998) Geomicrobiology: its significance for geology. Earth-Science Reviews, 45, 45–60.
Emmerich, K., Wolters, F., Kahr, G., and Lagaly, G. (2009) Clay profiling: the classification of montmorillonites. Clays and Clay Minerals, 57, 101–114.
Farmer, V.C. (1982) The Infrared Spectra of Minerals (Chinese version). Science Publishing House, Beijing.
Fortin, D., Ferris, F.G., and Beveridge, T.J. (1998) Surfacemediated mineral development by bacteria. Pp. l6l–180 in: Geomicrobiology: Interactions between Microbes and Minerals (J.F. Banfield and K.H. Nealson, editors). Reviews in Mineralogy, 35, Mineralogical Society of America, Washington, D.C.
Francesco, S. (1972) Structure and function of bacterial flagella. Bolletino di Zoologia, 39, 111–118.
Gates, W.P., Wilkinson, H.T., and Stucki, J.W. (1993) Swelling properties of microbially-reduced ferruginous smectite. Clays and Clay Minerals, 41, 360–364.
Glowa, K.R., Arocena, J.M., and Massicotte, H.B. (2003) Extraction of potassium and/or magnesium from selected soil minerals by Piloderma. Geomicrobiology Journal, 20, 99–111.
Harvey, C. and Lagaly, G. (2006) Conventional applications. Pp. 501–540 in: Handbook of Clay Science (F. Bergaya, B.K.G. Theng, and G. Lagaly, editors). Elsevier, Amsterdam.
Jaisi, D.P. (2007) Fe(III) reduction in clay minerals and its application to technetium immobilization. 319 pp. PhD dissertation, Miami University, Oxford, Ohio, USA.
Kim, J., Dong, H.L., Seabaugh, J., Newell, S.W., and Eberl, D.D. (2004) Role of microbes in the smectite-to-illite reaction. Science, 303, 830–832.
Kim, J., Furukawa, Y., Dong, H.L., and Newell, S.W. (2005) The effect of microbial Fe(III) reduction on smectite flocculation. Clays and Clay Minerals, 53, 572–579.
Kostka, J.E., Haefele, E., Viehweger, R., and Stucki, J.W. (1999) Respiration and dissolution of Fe(III)-containing clay minerals by bacteria. Environmental Science & Technology, 33, 3127–3133.
Li, X., Wu, Z. Q., Li, W.D., and Yan, R.X. (2007) Growth promoting effect of a transgenic Bacillus mucilaginosus on tobacco planting. Applied Microbial and Cell Physiology, 74, 1120–1125.
Lian, B. (1998) A study on how silicate bacteria GY92 dissolves potassium from illite. Acta Mineralogica Sinica, 18, 234–237.
Lian, B., Chen, Y., Zhao, J., Teng, H., Zhu, L.J., and Yuan, S. (2008) Microbial flocculation by Bacillus mucilaginosus: Applications and mechanisms. Bioresource Technology, 99, 4825–4831.
Liu, W.X., Xu, X.S., Yang, X.H., Luo, Y.M., and Christie, P. (2006) Decomposition of silicate minerals by Bacillus mucilaginosus in liquid culture. Environmental Geochemistryand Health, 18, 133–144.
Maurice, P.A., Vierkorn, M.A., Hersman, L.E., Fulghum, J.E., and Ferryman, A. (2001) Enhancement of kaolinite dissolution by an aerobic Pseudomonas mendocina bacterium. GeomicrobiologyJo urnal, 18, 21–35.
Monib, M., Zahra, M.K., and Abdel-Al, H.A. (1986) Role of silicate bacteria in releasing Kand Si from biotite and orthoclase. Soil Biologyan d Conservation of the Biosphere, 2, 733–743.
Richter, H., McCarthy, K., Nevin, K.P., Johnson, J.P., Rotello, V.M., and Lovley, D.R. (2008) Electricity generation by Geobacter sulfurreducens attached to gold electrodes. Langmuir, 24, 4376–4379.
Slosiarikova, H., Bujdak, J., and Hlavaty, V. (1992) IR spectra of octadecylammonium-montmorillonite in the range of the Si-O vibrations. Journal of Inclusion Phenomena and Molecular Recognition in Chemistry, 13, 267–272.
Srasra, E., Bergaya, F., and Fripiat, J.J. (1994) Infrared spectroscopy study of tetrahedral and octahedral substitutions in an interstratified illite-smectite clay. Clays and Clay Minerals, 42, 237–241.
Stucki, J.W. and Kostka, J.E. (2006) Microbial reduction of iron in smectite. Comptes Rendus Geoscience, 338, 468–475.
Stucki, J.W., Komadel, P., and Wilkinson, H.T. (1987) Microbial reduction of structural iron(III) in smectites. Soil Science Societyof America Journal, 51, 1663–1665.
Stucki, J.W., Lee, K., Zhang, L., and Larson, R.A. (2002) The effect of iron oxidation state on the surface and structural properties of smectite. Pure Applied Chemistry, 74, 2079–2092.
Sun, D., Zhang, X., and Zhang, Q. (2006) Leaching effects of metabolites of silicate bacterium on silicate minerals. Mining and Metallurgical Engineering, 26, 27–30.
The People’s Republic of China Agricultural Standards, NY882-2004 (2004) Silicate-dissolving bacteria culture.
Wu, J., Roth, C.B., and Low, P.F. (1988) Biological reduction of structural Fe in sodium-nontronite. Soil Science Society of America Journal, 52, 295–296.
Yin, X.P. (2005) The geological and application characteristics of bentonite deposit in Jianping. China Non-metallic Mining IndustryHer ald, 51, 56–58.
Zhang, G.X., Dong, H.L., Kim, J., and Eberl, D.D. (2007) Microbial reduction of structural Fe3+ in nontronite by a thermophilic bacterium and its role in promoting the smectite to illite reaction. American Mineralogist, 92, 1411–1419.
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Zhu, Y., Li, Y., Lu, A. et al. Study of the Interaction Between Bentonite and a Strain of Bacillus Mucilaginosus. Clays Clay Miner. 59, 538–545 (2011). https://doi.org/10.1346/CCMN.2011.0590511
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DOI: https://doi.org/10.1346/CCMN.2011.0590511