Colorectal cancer is the third most common cause of cancer mortality.1 Despite surgery and adjuvant therapies, half of patients have recurrences and ultimately die within 5 years from diagnosis.2 Patients with locoregional lymph node metastases (stage III) have an approximately 60% 5-year survival after surgery and adjuvant therapy, but when distant metastases are present the 5-year survival rate is less than 10%.3 The established adjuvant treatment in stage III patients was 5-fluorouracil (5-FU)-based chemotherapy at the time of the study, which has been reported to improve absolute 5-year survival at most by 10%.4,5 In stage II patients the statistically significant, absolute improvement in survival with chemotherapy is even smaller, and it is not generally recommended to all stage II patients.6 However, 20% of patients who are classified as being lymph node negative will suffer from recurrent disease.3 Attempts have been made to identify tumor characteristics conferring high risk of recurrence in order to offer adjuvant treatment exclusively to stage II patients with such tumors. Still, it is not clear whether this strategy is successful, and no consensus exists on which tumor characteristics are the most useful to predict relapse.

The most important negative prognostic factor for survival of colorectal cancer is the detection of lymph node or distant metastases, which are present in about 50% of all cases.7 Understaging is likely, since it is widely accepted that accurate staging of colon cancer is difficult and critically dependent on the number of lymph nodes analyzed by the pathologist.8 The sentinel node, originally defined in penile carcinoma, is the first lymph node to receive lymphatic drainage from a tumor.9 Sentinel node detection and analysis have recently been applied in colon cancer, improving staging considerably.10,11 To test if T-lymphocytes were components of a natural, tumor-evoked immune response originating in tumor-draining lymph nodes, we analyzed sentinel nodes from patients with urinary bladder cancer and colon cancer.1214 In those nodes we identified tumor-reactive lymphocytes, secreting interferon (IFN)-γ upon stimulation with tumor antigen in addition to dose-dependent proliferation, when assessed ex vivo directly after dissection. The sentinel node was the predominant location for activation and expansion of tumor-reactive lymphocytes.

Vaccination trials have shown promising results in colorectal cancer patients.1517 Adoptive immunotherapy, including collection and expansion of autologous tumor-reactive lymphocytes with retransfusion to the patient, has been explored in malignant melanoma, and recently a retroviral gene therapy approach using a melanoma-specific T-cell receptor has been tried.18,19 Immunotherapy offers an appealing complement to traditional chemotherapy, with possible long-term protection against tumor recurrences through immunological memory. Hitherto, the majority of studies have used peripheral blood or tumor-infiltrating lymphocytes as the source of CD8+ cytotoxic T lymphocytes for activation and expansion.

We became interested in the specific β-cell destruction observed in a mouse model, after transfer of in vitro β-cell surrogate antigen-activated CD4+ Th1-cells.20 In another mouse model, presence of CD4+ T-helper cells appeared necessary for the effective functioning of cytotoxic T-lymphocytes and for the development of memory cells.21 Furthermore, CD4+ T-helper-1 cells mature due to the expression of the transcription factor T-bet, which also induces production of IFN-γ necessary for effective defense against metastases.22,23 We reasoned that tumor-activated CD4+ Th1-cells harvested from the natural source, the tumor-draining sentinel node, may after in vitro expansion elicit a similar response in patients with disseminated cancer, eradicating metastatic cells which express antigens derived from the tumor. In the present study we obtained sentinel nodes from patients with disseminated or locally advanced, high-risk colon cancer with aggressive tumor characteristics such as ulcerations, vascular or perineural invasion. Sentinel or metinel (i.e., metastasis-draining lymph node) node-acquired lymphocytes were expanded ex vivo by stimulation with autologous tumor extract and retransfused into the patient with the primary objective of investigating feasibility, toxicity, and side-effects and secondarily to evaluate its effect on long-term tumor progression.14


Study Design

This was a pilot study, approved by the Local Ethical Committee at Karolinska Institute, Stockholm, Sweden. The inclusion criterion was operation due to metastatic or locally advanced, high-risk colon cancer, with peroperative sentinel node detection.


Sixteen consecutive patients diagnosed with colon cancer (six women and 10 men, average age 62 years) were included from September 2003 and followed with regular controls at the hospital until February 2008 (Table 1). Patients were informed both by surgeons and oncologists not participating in the study, and each patient gave informed consent. Patients were histopathologically classified as stage II, III or IV, as defined by American Joint Committee on Cancer (AJCC) criteria. All five patients with stage II disease were identified as high risk according to the vascular emboli, lymphatic invasion, and perineural invasion (VELIPI) criteria.24 They had aggressive tumor characteristics such as ulcerations, vascular or perineural invasion. Three patients (nos. 10, 13, and 15) had previously been treated for primary colon cancer and now had recurrent disease in the liver (nos. 10 and 13) or abdominally (no. 15). None of the patients had received any organ transplant, nor had a history of steroid treatment or other immunosuppressive regimes.

Table 1 Participant characteristics

Sentinel Node Detection

Identification of sentinel nodes was performed intraoperatively using the sentinel node technique.10 Mobilization of the colonic tumor or metastasis was conducted according to standard surgical procedures. Patent blue dye (Guerbet, Paris) was injected superficially in the serosa around the tumor or in the tissue surrounding the metastasis. One to three-first draining lymph nodes were colored blue, usually within 5 min. These sentinel nodes were marked with sutures and removed when the resection was complete (Fig. 1). Approximately half of each sentinel lymph node was retained for analysis and expansion, the other half being processed for routine histopathological examination by hematoxylin and eosin. A piece of the tumor or metastasis, including the invasive margin, was used for antigen preparation.

Fig. 1
figure 1

Perioperative identification of sentinel node(s). Identification of sentinel nodes was performed by subserosal injections of Patent blue dye at four sites around the tumor (a). Usually within 5 min, one or more blue-colored lymph nodes appear in the mesentery (b). Hypothetical scheme of activation of tumor-reactive lymphocytes in the sentinel node (c). In the tumor there is a rapid turnover of cells, and lack of oxygen and nutrition, causing a hostile environment which attracts macrophages and dendritic cells. These professional antigen-presenting cells (APC) phagocytose debris from tumor cells and then migrate via the lymph vessels to the draining sentinel node. In the sentinel node the APCs present tumor-derived peptides, thus activating tumor-reactive T-lymphocytes

Cell Culture

The sentinel node material was retained on ice and thereafter in AIM V® media (Invitrogen) in all subsequent steps. Single-cell suspensions of sentinel node lymphocytes were obtained through gentle homogenization in a loose-fit glass homogenizer, following which cells were washed twice in medium. The sentinel node lymphocytes were dispersed into cell culture flasks at a density of 4 million cells/ml and supplemented with interleukin-2 (IL-2) (Proleukin®, Chiron). Autologous tumor extract was prepared by homogenization with an Ultra Turrax in 5 volumes (w/v) of 2 × phosphate-buffered saline (PBS) followed by denaturation for 5 min at 97°C. Three to four days after initiation of the cell culture, autologous tumor extract was added at dilution of 1/100 (v/v). For long-term culture the cells were retained in a cell incubator at 37°C and 5% CO2, and IL-2 (Proleukin®, Chiron) was added every 3–4 days. After approximately 3 weeks the cell cultures were restimulated through the addition of autologous tumor extract at dilution of 1/100 (v/v). For efficient antigen presentation, autologous peripheral blood mononuclear cells (PBMC)were collected using Ficoll-Paque PLUS (Amersham Biosciences, GE Healthcare), irradiated with 2,500 rad, and added to the cell cultures. The day before transfusion, viable cells from the cultures were retrieved using Ficoll-Paque PLUS (Amersham Biosciences, GE Healthcare). On the day of transfusion, the cells were washed twice in saline solution (sodium chloride Baxter Viaflo 9 mg/ml, Baxter) and then transferred to a sterile plastic bag containing 100–200 ml saline solution and 1% human serum albumin (Baxter). Investigations to exclude microbial growth were performed prior to transfusion. Infusion of the cells back into the patient was performed according to the blood transfusion guidelines of the hospital.

Immunological Evaluation

The proliferative response upon stimulation with autologous tumor homogenate in PBMC was investigated using a tritium-labeled thymidine incorporation proliferation assay. PBMC were obtained by density centrifugation (Ficoll-Paque, Amersham Biosciences, GE Healthcare). Cells were resuspended and washed twice in Roswell Park Memorial Institute (RPMI) 1640 (Life Technologies) containing 2.5% fetal calf serum (FCS) (Life Technologies) before culture in RPMI 1640 medium containing 10% human AB serum (Sigma), 1% penicillin–streptomycin (Sigma), and 1% glutamine (Sigma). Lymphocytes were incubated at 3 × 105 cells/well in a 96-well plate and stimulated with tumor homogenate diluted 1/100 or 1/10 (v/v) or with the general T-lymphocyte mitogen Concanavalin A (Con A, 10 μg/ml; Sigma) in triplicate. Proliferation was measured on day 2 (Con A) and days 5, 6, and 7 (tumor homogenate) by adding 1 μCi 3H-thymidine/well (Amersham) 18 h prior to harvesting. Samples were subjected to scintillation counting. Stimulation of PBMC for measurement of IFN-γ secretion was performed in 96-well plates with 3 × 105 cells/well in triplicate with tumor homogenate diluted 1/10 and 1/100 (v/v), or Con A (10 μg/ml, Sigma). The amount of secreted IFN-γ was measured by enzyme-linked immunosorbent assay (ELISA, Human IFN-γ Duoset; R&D Systems) on culture supernatants in pooled triplicate samples. At the end of ex vivo culture of sentinel-node-acquired lymphocytes the amount of IFN-γ and IL-4 secreted into the supernatant was measured in triplicate by ELISA (Human IFN-γ Duoset and Human IL-4 Duoset, R&D Systems).

Flow Cytometry Analyses

Cells from the sentinel node, nonsentinel node, peripheral blood leukocytes (PBL), and from the tumor itself were characterized by fluorescence-activated cell sorting (FACS). Samples were analyzed by FACS regularly from the cultured sentinel-node-acquired lymphocytes. Cells were incubated for 30 min in PBS supplemented with 2% FCS and 0.05% NaN3 (FACS buffer) with antibodies against markers for immune cell subpopulations and for lymphocyte activation. Antibodies conjugated with fluorescein isothiocyanate (FITC) specific for the following markers were used: CD69, HLA-DR, CD45RA, CD25; conjugated with phycoerythrin (PE): CD62L, CD19, CD45RO, CD56, conjugated with peridinin-chlorophyll-protein (PerCP): CD8, CD3; conjugated with allophycocyanin (APC): CD4, CD14, CD8. The Vβ-TCR repertoire was examined using the Beta mark kit (Beckman Coulter), 5 × 105 cells/tube being stained in 10 μl of the eight different vials containing mixtures of FITC, PE, and dual-color FITC-PE-conjugated TCR Vβ antibodies. In addition, direct conjugated antibodies CD8 PerCP and CD4 APC were added to each tube. The presence of tumor cells was examined by intracellular staining for cytokeratin 20. Cells were permeabilized in FACS buffer containing 0.3% saponin, incubated for 30 min with anti-human cytokeratin 20 (Dako Cytomation), washed, and stained with FITC-conjugated donkey anti-mouse immunoglobulin G (IgG) for 30 min (Jackson ImmunoResearch).


Six patients (nos. 1, 3–5, 8, and 9) did not receive any chemotherapy. Patients 13 and 15 received chemotherapy after primary surgery. They later developed metastases (at least 1 year after chemotherapy) and at that time had sentinel-node-based immunotherapy, without any further chemotherapy. Patients 2, 6, 7, 10–12, 14, and 16 were treated with immunotherapy followed by chemotherapy (first-line 5-fluorouracil + leucovorin). Adverse effects related to chemotherapy and limited patient compliance led to early termination of chemotherapy treatment in four patients (nos. 6, 10, 12, and 14). Two stage IV patients received no chemotherapy treatment (nos. 8 and 9), and two patients received chemotherapy 1 year prior to immunotherapy (nos. 13 and 15).


Patients were clinically evaluated according to the regular follow-up program after diagnosis and treatment of colon cancer in Sweden. Follow-up visits usually took place 3, 6, 12, and 18 months after cell transfusion. On each of these occasions, general condition was estimated and the amount of carcinoembryonic antigen (CEA) in serum was measured. Every 6 months the patients were also examined for presence of liver and lung metastases by ultrasound, X-ray or computer tomography (CT) scan, or fluorodeoxyglucose positron emission tomography (FDG-PET). Assessment of clinical response was made by comparison of radiographic measurements and physical examinations before and after treatment using World Health Organization (WHO) criteria. Complete disappearance of all evaluable disease was defined as complete response (CR). Partial response (PR) was defined as decrease of 50% or more in the sum of the products of perpendicular diameters of all measurable lesions. Stable disease (SD) was defined as no signs of tumor progression between two consecutive CT evaluations of measurable remaining disease or lack of additional (stage IV) or no appearance of metastatic lesions (stage II and III).

Statistical Analysis

The number of cells received by patients with SD and PR disease was compared with the number of cells received by patients classified as CR using Wilcoxon’s rank-sum test. All patients in the Stockholm colon cancer registry diagnosed during the year 2003 with stage IV (n = 174) were used as a control group for cumulative survival comparison. The survival time of the nine stage IV patients treated with immunotherapy and the control stage IV cohort were plotted in an actuarial survival diagram. Survival times were compared using log-rank (Mantel–Cox) analysis. P < 0.05 was considered significant.


Expansion of Sentinel-Node-Acquired Lymphocytes

Sixteen patients with colorectal cancer were studied (Table 1). In all patients, 1–3 (average 2.1) sentinel node(s) were identified intraoperatively (Fig. 1) by peritumoral injections of Patent blue dye (Guerbet, Paris). We cultivated sentinel-node-acquired lymphocytes by stimulation with autologous tumor extract, to ensure clonal expansion of tumor-reactive CD4+ Th1-lymphocytes. Low amounts of interleukin-2 were added to overcome immunosuppression. The average number of sentinel-node-acquired lymphocytes at the start of expansion was 115.2 million cells (range 3.6–509 million, median 71 million). Cells were continuously monitored and characterized by flow cytometry (FACS) during expansion (Fig. 2a). The CD4+/CD8+ ratio at the start was 4.8 on average (range 0.36–10, median 5.0), indicating a natural enrichment of CD4+ T-helper lymphocytes in sentinel nodes when compared with the CD4/CD8 ratio in peripheral blood (range 1.0–2.5). In addition, B-lymphocytes (CD19+) and a small number of natural killer (NK) cells (CD56+, CD3) were present in sentinel nodes (not shown). The cells were held in culture for 36.5 days on average (range 23–58 days). Initially, the total number of cells decreased. B-lymphocytes disappeared almost completely (37.6% at start, 0.4% at transfusion), and the number of CD8+ T-cytotoxic cells diminished. This procedure mainly promoted the expansion of T-helper lymphocytes, since the average CD4/CD8 ratio at transfusion was 86.6 (Fig. 2a, Table 1). However, in some cases (Table 1) a substantial number of CD8+ T-cytotoxic lymphocytes developed. The transfused cells were heterogenous with respect to CD62L expression, but uniformly CD45RO positive, indicative of a mixed memory-cell population, comprising both central and effector memory cells (Fig. 2a).

Fig. 2
figure 2

Sentinel node lymphocytes are enriched in T-helper cells and secrete IFN-γ after ex vivo cell culturing. At the start and end of ex vivo cell culture, sentinel-node-acquired lymphocytes were characterized by flow cytometry with regards to activation and memory cell markers (a). The amount of IFN-γ and IL-4 secreted into the supernatant was measured by ELISA (b). The data presented is from patient 15 on the day prior to transfusion, which in this case corresponds to 40 days of ex vivo cell culture. T-cell receptor Vβ repertoire of expanded sentinel-node-acquired lymphocytes (c). In patient 7 the T-cell receptor Vβ repertoire was investigated by flow cytometry at the start and end of ex vivo cell culturing. Clonal (Vβ 22) expansion of CD4+ T-helper lymphocytes was detected

ELISA Results

In six cases (Table 1), the expanded T-lymphocytes were functionally tested before transfusion by measuring the levels of the Th1 cytokine IFN-γ and the Th2 cytokine IL-4 in the cell culture supernatants (Fig. 2b). The average level of IFN-γ was 956 pg/ml and the average level of IL-4 was 11 pg/ml, indicating that the expanded T- lymphocytes were functional and Th1 responsive. Restimulation after about 3 weeks with autologous tumor antigen and autologous PBMCs, acting as antigen-presenting cells, resulted in further clonal expansion of tumor-reactive T-lymphocytes as assessed by investigating the T-cell receptor Vβ repertoire of sentinel-node-acquired lymphocytes before and after restimulation (Fig. 2c).

Retransfusion and Toxicity

On average, 71 million clonally expanded autologous tumor-reactive T-lymphocytes were retransfused to each patient (Table 1). No toxic side-effects, such as fever, chills, malaise, severe fluid retention, pulmonary edema or respiratory distress, were observed.

Clinical Outcome

The patients were followed for 36 months on average (range 6–51 months) and monitored in accordance with the Swedish colorectal cancer follow-up protocol. The patients with distant metastases (stage IV) responded to treatment, with either extended periods of stable disease (n = 4), partial response with diminished tumor burden (n = 1) or complete response with no detectable remaining tumor (n = 4) (Table 1). Patient no. 14 (Table 1) with disseminated disease had a large sigmoid tumor resected en bloc, involving the left ovary, the left uterine tube, and the upper part of the uterus. She had metastases in both liver lobes. The metastases regressed completely after immunotherapy, as demonstrated by CT scan (Fig. 3a) and FDG-PET (Fig. 3b). Patient no. 12 (Table 1) displayed disseminated disease with large liver metastases and multiple lung metastases. The primary tumor was resected because of obstructive symptoms, and immunotherapy followed after 5 weeks. Partial response with marked regression of liver metastases and disappearance of several lung metastases occurred within 3 months (Fig. 3c). Notably, the stage IV patients responding with SD and PR received significantly fewer cells than the four patients with CR (P < 0.05) (Fig. 3d), thus indicating a dose response.

Fig. 3
figure 3

Patient outcome. Computer tomography (CT) of patient 14, showing a metastasis measuring 12.7 × 13.0 × 13.3 mm3 in the liver at time of treatment (a, left panel). Follow-up CT scan 3 months after treatment showed no signs of liver metastasis. (a, right panel). FDG-PET of patient 14 at time of treatment revealed additional FDG-positive metastases in the liver, indicated by arrows (b, left panel). Follow-up FDG-PET scan 6 months after treatment without any signs of FDG-positive metastases, indicating complete remission; the only remaining FDG uptake is seen in the kidneys (b, right panel). Imaging of lung metastases. In patient 12, bilateral lung metastases were detected on computer tomography (CT) prior to surgery (c, left panel). Follow-up CT scan 3 months after cell transfusion revealed complete remission of some metastases and significant decrease in the remaining metastases (c, right panel). Dose-dependent response in the stage IV patients (d). Complete response was seen in four of the nine stage IV patients. Patients with complete response were transfused with an average of 150 million cells, a significantly (P < 0.05) larger number of cells than the average of 27 million cells transfused in patients with partial response or stable disease

The cumulative survival of the nine treated stage IV patients was compared with all stage IV cases in the Stockholm region during the year of 2003 (n = 174). The median survival of stage IV patients who received immunotherapy was 2.6 years compared with 0.8 years median survival of the control group, and there was a tendency towards increased cumulative survival (P = 0.06) (Fig. 4e). Six patients have died, of whom five were classified as responders with stable disease (nos. 8 and 11), partial response (no. 12) or complete response (nos. 13 and 14). Patient 9 was lost to follow-up. Patients no. 13 and 14 were classified as complete responders after immunotherapy. However, patient 14 developed metastases to the liver after 3 years and died after 40 months. Patient no. 13 died from pancreatic cancer without any signs of recurrent colon cancer. Details regarding dead patients are presented in Table 1.

Fig. 4
figure 4

Treatment responses after sentinel-node-based adoptive immunotherapy. Immune response upon stimulation with autologous tumor extract could be detected in peripheral blood leukocytes from patient 10 (a and b). Patient 10 had, 10 months earlier, received a transfusion of ex vivo expanded sentinel node lymphocytes. The proliferative response (a) was measured by 3H-thymidine incorporation assay (day 5), and the amount of IFN-γ secreted into the supernatant was measured by ELISA (day 6) (b). Stimulation with autologous tumor extract diluted 1/100 or 1/10 or in medium alone (0). In patient 16 the proliferative response in peripheral blood after transfusion, at 10 months (c) and 42 months (d), was investigated by 3H-thymidine incorporation assay. At 10 months, peak proliferation upon stimulation with autologous tumor homogenate (diluted 1/100 and 1/10) was approximately 10,000 cpm (day 7) (c). By 42 months the peak proliferation had increased to 47,000 cpm (day 6) upon addition of autologous tumor homogenate (diluted 1/100 and 1/10) (d). Proliferation in response to addition of allogeneic tumor homogenate from patient 5 was also investigated (“CC pat 5 1/100” and “CC pat 5 1/10”), resulting in peak proliferation of 31,000 cpm (day 6) (d). Increased survival in stage IV patients treated with sentinel-node-based adoptive immunotherapy (e). Actuarial survival curves were obtained by comparing survival among patients with stage IV receiving adoptive immunotherapy (9 patients) versus historical controls diagnosed in the year 2003 (174 cases)

Five patients classified as stage II, with high-risk tumor characteristics, were included in the study (Table 1). One stage II patient (no. 2) developed liver recurrences, which responded to immunotherapy with regression of metastases. He is now free of recurrences and classified as having stable disease. The other patients with high-risk stage II disease (nos. 1 and 3–5) and the patients with stage III (nos. 6 and 7) have had no signs of recurrences and are thus classified as stable disease according to their original status.

Posttreatment Immunological Evaluation

We tested peripheral blood leukocytes for their memory capacity by proliferation assays in two patients (nos. 10 and 16) by recalling reactivity against autologous tumor extract. Ten months after transfusion we detected proliferation and secretion of IFN-γ in response to autologous tumor extract (Fig. 4a–c), indicating the presence of memory cells. Peripheral blood leukocytes from patient no. 16 were again tested for proliferation against autologous tumor extract 42 months after treatment. Interestingly an increased recall proliferation was noticed after 42 months (47,000 cpm) as compared with responses seen after 10 months (10,000 cpm) (Fig. 4c, d), suggesting that expansion of a long-lived memory cell population occurred in vivo. When these cells were tested for proliferation against allogeneic tumor extract from patient 5, a recall response was noted, albeit at a lower level, suggesting that common tumor antigens are present in these tumor preparations (Fig. 4d).


We have previously identified the localized presence of immune responses against the autologous tumor in sentinel nodes draining colorectal and urinary bladder cancers.1214 In the present study, sentinel-node-acquired lymphocytes were expanded ex vivo against an autologous tumor extract and retransfused to 16 patients with colorectal cancer.

In theory, processing and presentation of exogenous intact antigens via endocytosis favors loading of the major histocompatibility complex (MHC) class II pocket, thus mediating clonal expansion of class II restricted tumor-reactive CD4+ T-helper lymphocytes. This also appeared to be the case, as evidenced by an increased CD4/CD8 ratio in the majority of cell cultures (Table 1). However, in some cases a significant expansion of CD8+ cytotoxic T-cells was observed, most likely the result of cross-presentation of extracellular antigens on MHC class I molecules.25 The expanded cell populations displayed the surface phenotype of memory cells (Fig. 2a) and a cytokine secretion profile of Th1-cells (Fig. 2c). Both central (CD62L-positive) and effector (CD62L-negative) cells were present in the transfused cell populations (Fig. 2a). Whereas effector memory cells are capable of executing immediate effector functions upon antigen encounter, central memory cells home to lymph nodes and may provide a lifelong source of new effector cells, both upon secondary stimulation and under the influence of homeostatic cytokines.26,27 Indeed, both proliferation and IFN-γ secretion in response to tumor extract could be detected in peripheral blood 10–42 months after treatment (Fig. 4a–d).

On average, 71 million clonally expanded lymphocytes were used in the adoptive transfers, which is a relatively small number compared with protocols using primarily CD8+ cytotoxic T-cells.28 We consider this justifiable, since as few as 3 × 105 adoptively transferred, antigen-specific CD4+ Th1-cells are able to mediate tumor regression in mice, mainly through recruitment and expansion of host CD8+ T-cells.29 Indeed, four patients with distant metastases (stage IV) displayed complete tumor regression after treatment with sentinel-node-acquired lymphocytes. However, the five stage IV patients with PR or SD all received lymphocytes in the lower range (Fig. 3a), suggesting overwhelming immunosuppression from their residual metastatic disease. Even when an antitumoral response is induced by transfer of tumor-reactive CD4+ Th1-lymphocytes, tumor cells may escape elimination by losing targeted antigens, rendering T-cells anergic by downregulation of costimulatory molecules, by inducing regulatory T-lymphocytes, or by specifically deleting responding T-lymphocytes.3033 The tumor that finally develops may reflect a selection of poorly immunogenic and/or immune-resistant malignant cells.34 Tumor-induced immunomodulation may not be limited to the tumor microenvironment. Indeed, immunosuppression has been identified also in lymphocytes from sentinel nodes, which has been suggested to facilitate lymph node metastases.35 However, our method appears to reconstitute the function of tumor-reactive CD4+ Th1-lymphocytes and provide a large number of functioning tumor-reactive T-lymphocytes, resulting in large antitumoral responses in patients receiving large numbers of tumor-reactive T-cells. Thus, an iterated therapy may be advocated, especially in the presence of a significant tumor burden.

Life expectancy in stage IV patients is 8–12 months.36 Even though the cumulative survival was not significantly different from in conventionally treated controls, likely due to the limited number of patients included in our study, the median survival of 2.6 years in the nine immunotherapy-treated stage IV patients is encouraging (Fig. 4e). The possibility of receiving immunotherapy tended to select patients hesitant to subject themselves to chemotherapy, and therefore the overall amount of chemotherapy used was low. Most patients received only immunotherapy (as described in the “Methods” section). Among the four complete responders, two patients (nos. 14 and 16) received chemotherapy in association with immunotherapy, whereas the other two patients had chemotherapy over 1 year prior to immunotherapy (patients no. 13 and 15). Thus, the clinical results among immunotherapy-treated stage IV patients are unlikely due to chemotherapy. Theoretically, chemotherapy is a two-edged sword. Chemotherapy may decrease the proliferative capability of tumor-reactive T-cells since chemotherapy affects dividing cells, and therefore results may have been underestimated. On the other hand, when chemotherapy is administered, the increased tumor cell death will result in increased antigen presentation to T-lymphocytes, thus increased T-cell reactivity may occur, as discussed in a recent review.37 It would be interesting to test immunotherapy and chemotherapy in combination in a future systematic study.

To our knowledge, sentinel-node-based immunotherapy in patients with colon cancer is a novel approach. There are some major differences between our study and others that use anti-CD3/anti-CD28 expanded tumor-infiltrating lymphocytes, myeloablation, and high-dose IL-2.28 Firstly, we use sentinel-node-acquired lymphocytes providing a natural antitumoral response, enhanced by ex vivo stimulation with autologous tumor homogenate, physiological doses of IL-2, and restimulation with autologous antigen-presenting cells (APCs) on one occasion after about 3 weeks of expansion. Only T-lymphocytes with high affinity to antigens from the primary tumor will survive until transfusion. Secondly, myeloablation and high-dose IL-2 regimen entail high toxicity and bring along risk of severe complications, long treatment periods, and high costs. Our transfusions, however, were given without complications, during a period of 1 h, with patients often discharged the same day. Thirdly, our protocol aims at expansion of CD4+ Th1-lymphocytes from sentinel nodes, as opposed to expansion of cytotoxic CD8+ T-lymphocytes harvested as tumor-infiltrating lymphocytes.

In a recent retrospective analysis of resected tumors and lymph nodes from patients with colorectal cancer, the influence of immune cells on the advancement and outcome of disease was investigated.24 Increased numbers of memory effector cells, Th1 activation as indicated by increased messenger RNA (mRNA) for IFN-γ, as well as presence of the Th1-inducing transcription factor T-bet within the tumor were all associated with absence of early metastatic invasion and correlated strongly with prolonged survival. Notably, the patients in our study were treated with the same population of Th1-lymphocytes. Interestingly, a recent experimental mouse model supports the efficacy of CD4+ T-helper lymphocytes in adoptive immunotherapy. Mice with established tumors were treated with adoptive transfer using in vitro activated T-lymphocytes harvested from tumor-draining lymph nodes. The results demonstrated that expanded sorted CD4+ T-helper lymphocytes from the tumor-draining lymph node have therapeutic efficacy on their own, and a synergistic effect was found when CD4+ T-helper lymphocytes were used in combination with expanded cytotoxic CD8+ T-lymphocytes.38 In addition, adoptive transfer of a tumor antigen-specific CD4+ T-cell clone to one patient with metastatic malignant melanoma was recently reported to induce a 2-month clinical remission.39 In the light of these findings, future trials using sentinel-node-acquired CD4+ and CD8+ T-lymphocytes, raised separately under optimal conditions, may further improve the outcome of sentinel-node-based adaptive immunotherapy.

In conclusion, our study shows the novel finding that freshly isolated sentinel-node-acquired lymphocytes possess proliferative ability ex vivo against autologous tumor homogenate and may be retransfused to the patient without complications, as adoptive immunotherapy. Our results indicate that this cell population is capable of inducing tumor regression in patients with disseminated colorectal cancer. Due to its generic design, i.e., identification of sentinel node, isolation of tumor-reactive lymphocytes, and ex vivo expansion, this method may be used to treat other types of disseminated solid cancers.