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Development and Characterization of Bioadhesive Film Embedded with Lignocaine and Calcium Fluoride Nanoparticles

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Abstract

The formation of biofilm by Streptococcus mutans on the tooth surface is the primary cause of dental caries and periodontal diseases, and fluoride (F) has shown tremendous potential as a therapeutic moiety against these problems. Herein, we report an efficient multi-ingredient bioadhesive film-based delivery system for oral cavity to combat dental problems with an ease of administration. Thiolated chitosan-based bioadhesive film loaded with calcium fluoride nanoparticles (CaF2 NPs) and lignocaine as a continuous reservoir for prolonged delivery was successfully prepared and characterized. The polygonal CaF2 NPs with an average particle size less than 100 nm, PDI 0.253, and + 6.10 mV zeta potential were synthesized and loaded in film. The energy dispersive x-ray (EDX) spectroscopy confirmed the presence 33.13% F content in CaF2 NPs. The characterization of the three film trials for their mechanical strength, bioadhesion, drug release, and permeation enhancement suggested film B as better among the three trials and showed significant outcomes, indicating the potential application of the medicated bioadhesive film. In vitro dissolution studies revealed sustained release pattern of lignocaine and CaF2 NP following Krosmeyer-Peppas model over 8 h. Franz diffusion studies showed the prolonged contact time of film with mucosa that facilitated the transport of CaF2 NPs and lignocaine across the mucosa. Hence, the prepared bioadhesive film-based system showed good potential for better management of dental problems.

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Acknowledgments

The authors extend their gratitude to NovaMed Pharmaceuticals Pvt. Ltd. Pakistan, Government College University, Lahore, Pakistan, and Lahore University of Management Sciences, Lahore, Pakistan, for facilitation the research process.

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Authors and Affiliations

  1. Riphah Institute of Pharmaceutical Sciences (RIPS), Riphah International University, Lahore Campus, Lahore, Pakistan

    Hira Ghafar, Muhammad Imran Khan, Hafiz Shoaib Sarwar, Samiya Yaqoob & Muhammad Farhan Sohail

  2. Department of Chemistry and Chemical Engineering, Lahore University of Management Sciences (LUMS), Lahore, Pakistan

    Syed Zajif Hussain

  3. Department of Pharmaceutics and Biopharmaceutics, Philipps University Marburg, Marburg, Germany

    Imran Tariq

  4. Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

    Asad Ullah Madni

  5. Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Gul Shahnaz & Muhammad Farhan Sohail

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  1. Hira Ghafar
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  2. Muhammad Imran Khan
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Correspondence to Muhammad Farhan Sohail.

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Animal studies were conducted after approval from the institutional ethical committee of Riphah International University.

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Ghafar, H., Khan, M.I., Sarwar, H.S. et al. Development and Characterization of Bioadhesive Film Embedded with Lignocaine and Calcium Fluoride Nanoparticles. AAPS PharmSciTech 21, 60 (2020). https://doi.org/10.1208/s12249-019-1615-5

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