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Pectin and Mucin Enhance the Bioadhesion of Drug Loaded Nanofibrillated Cellulose Films

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Abstract

Purpose

Bioadhesion is an important property of biological membranes, that can be utilized in pharmaceutical and biomedical applications. In this study, we have fabricated mucoadhesive drug releasing films with bio-based, non-toxic and biodegradable polymers that do not require chemical modifications.

Methods

Nanofibrillar cellulose and anionic type nanofibrillar cellulose were used as film forming materials with known mucoadhesive components mucin, pectin and chitosan as functional bioadhesion enhancers. Different polymer combinations were investigated to study the adhesiveness, solid state characteristics, film morphology, swelling, mechanical properties, drug release with the model compound metronidazole and in vitro cytotoxicity using TR146 cells to model buccal epithelium.

Results

SEM revealed lamellar structures within the films, which had a thickness ranging 40–240 μm depending on the film polymer composition. All bioadhesive components were non-toxic and showed high adhesiveness. Rapid drug release was observed, as 60–80% of the total amount of metronidazole was released in 30 min depending on the film formulation.

Conclusions

The liquid molding used was a straightforward and simple method to produce drug releasing highly mucoadhesive films, which could be utilized in treating local oral diseases, such as periodontitis. All materials used were natural biodegradable polymers from renewable sources, which are generally regarded as safe.

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Abbreviations

NFC:

Nanofibrillar cellulose

ANFC:

Anionic type nanofibrillar cellulose

MZ:

Metronidazole

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Acknowledgements

The financial support from Academy of Finland (Grant No. 258114) is gratefully acknowledged. Orion Foundation of the Professor Pool, Finland is greatly acknowledged.

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Author notes

  1. Patrick Laurén and Heli Paukkonen contributed equally to this work.

Authors and Affiliations

  1. Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Patrick Laurén, Heli Paukkonen, Timo Oksanen, Marjo Yliperttula & Timo Laaksonen

  2. Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Tiina Lipiäinen, Heikki Räikkönen & Henrik Ehlers

  3. Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland

    Yujiao Dong & Päivi Laaksonen

  4. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Marjo Yliperttula

  5. Laboratory of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland

    Timo Laaksonen

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  1. Patrick Laurén
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  2. Heli Paukkonen
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Correspondence to Timo Laaksonen.

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Laurén, P., Paukkonen, H., Lipiäinen, T. et al. Pectin and Mucin Enhance the Bioadhesion of Drug Loaded Nanofibrillated Cellulose Films. Pharm Res 35, 145 (2018). https://doi.org/10.1007/s11095-018-2428-z

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  • DOI: https://doi.org/10.1007/s11095-018-2428-z

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