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Development and Characterization of Propranolol Selective Molecular Imprinted Polymer Composite Electrospun Nanofiber Membrane

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Abstract

Propranolol (PPL) imprinted microspheres (MIP) were successfully prepared via oil/water polymerization using a methyl methacrylate (MMA) monomer, PLL template, and divinylbenzene (DVB) cross-linker and favorably incorporated in a Eudragit-RS100 nanofiber membrane. A non-PPL imprinted polymer (NIP), without a template, was used as a control. The morphology and particle size of the beads were investigated using scanning electron microscopy. The results revealed that both MIP and NIP had a spherical shape with a micron size of approximately 50–100 μm depending on the amounts of DVB and PPL used. NIP2 (MMA/DVB, 75:2.5) and MIP8 (PPL/MMA/DVB, 0.8:75:2.5) were selected for reloading of PPL, and the result indicated that increasing the ratio of PPL to polymer beads resulted in increase PPL reloading (>80%). A total of 10–50% NIP2 or MIP8 was incorporated into a 40% (w/v) Eudragit-RS100 fiber membrane using an electrospinning technique. PPL could be bound to the 50% MIP8 composite fiber membrane with a higher extent and at a higher rate than the control (NIP2). Furthermore, the MIP8 composite fiber membrane showed higher selectivity to PPL than the other β-blockers (atenolol, metoprolol, and timolol). Thus, the MIP8 composite fiber membrane can be further developed for various applications in pharmaceutical and other affinity separation fields.

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References

  1. Ma Z, Kotaki M, Ramakrishna S. Surface modified nonwoven polysulphone (PSU) fiber mesh by electrospinning: a novel affinity membrane. J Membr Sci. 2006;272(1–2):179–87.

    Article  CAS  Google Scholar 

  2. Ma Z, Kotaki M, Ramakrishna S. Electrospun cellulose nanofiber as affinity membrane. J Membr Sci. 2005;265(1–2):115–23.

    Article  CAS  Google Scholar 

  3. Borcherding H, Hicke H, Jorcke D, Ulbricht M. Surface functionalized microfiltration membranes for affinity separation. Desalination. 2002;149(1–3):297–302.

    Article  CAS  Google Scholar 

  4. Mosbach K, Ramstrom O. The emerging technique of molecular imprinting and its future impact on biotechnology. Nat Biotechnol. 1996;14:163–70.

    Article  CAS  Google Scholar 

  5. Ye L, Haupt K. Molecularly imprinted polymers as antibody and receptor mimics for assays, sensors and drug discovery. Anal Bioanal Chem. 2004;378:1887–97.

    Article  CAS  PubMed  Google Scholar 

  6. Ye L, Weiss R, Mosbach K. Synthesis and characterization of molecularly imprinted microspheres. Macromolecules. 2000;33:8239–45.

    Article  CAS  Google Scholar 

  7. Vlatakis G, Andersson LI, Miller R, Mosbach K. Drug assay using antibody mimics made by molecular imprinting. Nature. 1993;361:645–7.

    Article  CAS  PubMed  Google Scholar 

  8. Yan H, Row KH. Characteristic and synthetic approach of molecularly imprinted polymer. Int J Mol Sci. 2006;7:155–78.

    Article  CAS  Google Scholar 

  9. Lubke C, Lubke M, Whitcombe MJ, Vulfson EN. Imprinted polymers prepared with stoichiometric template–monomer complexes: efficient binding of ampicillin from aqueous solutions. Macromolecules. 2000;33:5098–105.

    Article  Google Scholar 

  10. Yao LD, Tang YW, Huang ZF. Nicotinic acid voltammetric sensor based on molecularly imprinted polymer membrane-modified electrode. Anal Lett. 2007;40:677–88.

    Article  CAS  Google Scholar 

  11. Haupt K, Mosbach K. Molecularly imprinted polymers and their use in biomimetic sensors. Chem Rev. 2000;100:2495–504.

    Article  CAS  PubMed  Google Scholar 

  12. Kriz O, Ramstrom O, Mosbach K. Molecular imprinting: new possibilities for sensor technology. Anal Chem. 1997;69:345A–9.

    Article  CAS  Google Scholar 

  13. Ansell RJ, Kriz D, Mosbach K. Molecularly imprinted polymers for bioanalysis: chromatography, binding assays and biomimetic sensors. Curr Opin Biotechnol. 1996;7:89–94.

    Article  CAS  PubMed  Google Scholar 

  14. Liu HY, Row KH, Yan GL. Monolithic molecularly imprinted columns for chromatographic separation. Chromatographia. 2005;61:429–32.

    Article  CAS  Google Scholar 

  15. Hwang CC, Lee WC. Chromatographic resolution of the enantiomers of phenylpropanolamine by using molecularly imprinted polymer as the stationary phase. J Chromatogr B. 2001;765:45–53.

    Article  CAS  Google Scholar 

  16. Martin PD, Jones GR, Stringer F, Wilson ID. Comparison of normal and reversed-phase solid phase extraction methods for extraction of β-blockers from plasma using molecularly imprinted polymers. Analyst. 2003;128:345–50.

    Article  CAS  PubMed  Google Scholar 

  17. Lavignac N, Allender CJ, Brain KR. Current status of molecularly imprinted polymers as alternatives to antibodies in sorbent assays. Anal Chim Acta. 2004;510:139–45.

    Article  CAS  Google Scholar 

  18. Ye L, Mosbach K. Molecularly imprinted microspheres as antibody binding mimics. React Funct Polym. 2001;48:149–57.

    Article  CAS  Google Scholar 

  19. Chianella I, Lotierzo M, Piletsky SA, Tothill IE, Chen B, Karim K, et al. Rational design of a polymer specific for microcystin-LR using a computational approach. Anal Chem. 2002;74:1288–93.

    Article  CAS  PubMed  Google Scholar 

  20. Son SH, Jegal J. Chiral separation of D, L-serine racemate using a molecularly imprinted polymer composite membrane. J Appl Polym Sci. 2007;104:1866–72.

    Article  CAS  Google Scholar 

  21. Zaidi SA, Cheong WJ. Robust open tubular layer of S-ketoprofen imprinted polymer for chiral LC separation. J Sep Sci. 2008;31:2962–70.

    Article  CAS  PubMed  Google Scholar 

  22. Yoshimatsu K, Ye L, Lindberg J, Chronakis IS. Selective molecular adsorption using electrospun nanofiber affinity membranes. Biosens Bioelectron. 2008;23:1208–15.

    Article  CAS  PubMed  Google Scholar 

  23. Bhardwaj N, Kundu SC. Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv. 2010;28:325–47.

    Article  CAS  PubMed  Google Scholar 

  24. Bamford CH, Al-Lamee KG, Pm-brick MD, Wear TJ. Studies of a novel membrane for affinity separations. I. Functionalisation and protein coupling. J Chromatogr. 1992;606:19–31.

    Article  CAS  Google Scholar 

  25. Ongun MZ, Ertekin K, Gocmenturk M, Ergun Y, Suslu A. Copper ion sensing with fluorescent electrospun nanofibers. Spectrochim Acta A Mol Biomol Spectrosc. 2012;90:177–85.

    Article  CAS  PubMed  Google Scholar 

  26. Charernsriwilaiwat N, Opanasopit P, Rojanarata T, Ngawhirunpat T. Lysozyme-loaded, electrospun chitosan-based nanofiber mats for wound healing. Int J Pharm. 2012;427:379–84.

    Article  CAS  PubMed  Google Scholar 

  27. Yoshimatsu K, Reimhult K, Krozer A, Mosbach K, Sode K, Ye L. Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: the control of particle size suitable for different analytical applications. Anal Chim Acta. 2007;584:112–21.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors would like to acknowledge Commission of Higher Education (Thailand) and the Thailand Research Funds through the Golden Jubilee Ph.D. Program (grant no. PHD/0092/2554) and the Silpakorn University Research and development institute for the financial support (grant no. SURID55/02/12).

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Authors and Affiliations

  1. Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand

    Prasopchai Tonglairoum, Wanita Chaijaroenluk, Theerasak Rojanarata, Tanasait Ngawhirunpat, Prasert Akkaramongkolporn & Praneet Opanasopit

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  1. Prasopchai Tonglairoum
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  2. Wanita Chaijaroenluk
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  3. Theerasak Rojanarata
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  4. Tanasait Ngawhirunpat
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  5. Prasert Akkaramongkolporn
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  6. Praneet Opanasopit
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Correspondence to Praneet Opanasopit.

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Tonglairoum, P., Chaijaroenluk, W., Rojanarata, T. et al. Development and Characterization of Propranolol Selective Molecular Imprinted Polymer Composite Electrospun Nanofiber Membrane. AAPS PharmSciTech 14, 838–846 (2013). https://doi.org/10.1208/s12249-013-9970-0

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