Correction
The original article [1] contains an omission in the Methods. The authors would like to note that the paragraph constituting the L1 promoter assay should read as the following:
L1 promoter assay
HEK-293T cells were transfected with the L1 promoter luciferase reporter plasmid (L1RP-luc) together with empty vector (pcDNA6mycHis) or SAMHD1 expressing vector using calcium phosphate. The reporter plasmid L1RP-luc and the L1 promoter activity assay have been described previously [2]. Two days posttransfection, cells were lysed with Cell Culture Lysis 5× reagent (Promega) and luciferase activity was quantified using commercially available components (Promega).
References
Herrmann A, et al. The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation. Mob DNA. 2018;9:11.
Yu F, Zingler N, Schumann G, Strätling WH. Methyl-CpG-binding protein 2 represses LINE-1 expression and retrotransposition but not Alu transcription. Nucleic Acids Res. 2001;29:4493–501.
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Herrmann, A., Wittmann, S., Thomas, D. et al. Correction to: The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation. Mobile DNA 9, 16 (2018). https://doi.org/10.1186/s13100-018-0121-8
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DOI: https://doi.org/10.1186/s13100-018-0121-8