In recent years, much attention has been given to the debate as to whether malaria elimination is a viable and realistic option for malaria-endemic countries [13], whether the tools currently available are able to achieve elimination, and if elimination is a sensible strategy for control programmes [1]. What is clear is that metrics suited to lower transmission levels will be needed to allow accurate evaluation of control and elimination programmes [4]. The performance of different metrics needs to be characterized in a variety of medium and low transmission settings to ensure that the heterogeneity of malaria burden can be accurately described, targeted and monitored across operationally appropriate geographical scales.

Microscopy has been the historic mainstay of the estimation of infection prevalence. More recently infection prevalence has been assessed by rapid diagnostic tests (RDT). Both approaches have a similar sensitivity with limits of detection in the order of 5–50 parasites/μl [4]. Whilst the operational utility of these measures, particularly RDT, cannot be discounted, sufficient data have accumulated to suggest that they significantly underestimate the level of infection [57]. Molecular methods based on PCR of DNA detect 0.5–5 parasites/μl and reports suggest on average twofold more infections than that detected by microscopy [6, 8]. This difference is more pronounced in older individuals and in lower transmission settings and appears to be related in part to immunity and parasite density [5, 9]. The relevance of these low-density, sub-patent infections is that they can still infect mosquitoes. Furthermore, despite the typically lower density parasitaemia, the chronic and widespread nature of these infections suggests longer periods of infectivity, which therefore act as an important source of transmission especially in areas of high vector competence [5, 10].

In low transmission settings, the burden of malaria shifts with infections distributed across age groups. There have been few studies comparing the utility of different diagnostics in these settings [68, 11, 12]. There is increasing evidence that serological measures of exposure (i.e., the detection of antibody responses specific for malaria parasites) can provide an additional, more sensitive measure of transmission in these low-endemic areas. Antibody responses measured as simple prevalence in Somaliland showed that almost one-fifth of people had serological evidence of exposure despite the absence of parasites by microscopy and that exposure as determined by antibody levels was associated with travel to nearby Ethiopia [13]. At larger scales, the seroconversion rate (SCR) calculated from age and antibody prevalence has been shown to correlate with other measures of transmission intensity and therefore may be more useful in low-endemic settings [4, 14].

To further understand malaria dynamics in a low transmission setting, a cross-sectional survey was carried out in a highland fringe area of western Kenya (altitude 1400–1600 m). Whilst several studies have reported malaria transmission from either lowland or highland areas of Nyanza Province [1520], few have been carried out between these two extremes. The aims of the surveys were to (1) characterize the epidemiology of Plasmodium falciparum in this setting; (2) to assess the utility of different diagnostic tools, including RDTs, PCR, and the presence of antibodies to the parasite antigen MSP1-19; and, (3) to identify factors associated with malaria infections/exposures based on the three malaria metrics used.


Study site and survey procedure

Malaria surveys were conducted in the neighbouring highland districts of Kisii Central, Kisii South and Rachuonyo South, Nyanza Province, western Kenya as part of the Malaria Transmission Consortium project [21]. The total population of the study districts at the time of the 2009 national census was 863,000. The area is predominantly rural with subsistence agriculture being the main occupation. People of the Kisii and Luo ethnic group predominantly occupy Kisii Central and Kisii South (henceforth referred to as Kisii) and Rachuonyo, respectively. Malaria transmission is seasonal with two peaks following the bimodal rainfall pattern, the heaviest rainfall typically occurring between March and June, with a smaller peak in October/November each year. The main malaria vectors are Anopheles funestus and Anopheles arabiensis, and P. falciparum is the predominant malaria parasite.

Sample size was calculated to adequately define the prevalence of P. falciparum infection among different age groups with 95 % confidence, with 80 % power, and assuming a design effect of 2. Based on historical prevalence data for the field area and its vicinity, the prevalence of infection was assumed to be 15 % among children under 5 years of age (the smallest population age group being considered in this study). Assuming an average household size of six persons and a 15 % non-response rate, a total sample of 1273 people per district was required to in order to estimate age-specific prevalence to a precision of ±3 %.

Survey compounds (which represent a family homestead of one or more houses) were selected using a two-stage sampling design. At the first stage, 46 enumeration areas (EAs, the primary sampling unit) were selected randomly from a sampling frame restricted to EAs with a mean altitude of 1400–1600 m (Fig. 1). EAs were defined by the Kenya National Bureau of Statistics during the 2009 national census to demarcate areas with approximately 500 residents. Urban EAs and EAs with boundaries contiguous with the main Rachuonyo-Kisii boundary were excluded.

Fig. 1
figure 1

Map highlighting the location of the randomly selected enumeration areas included in the survey

All compounds in selected EAs were geolocated and enumerated. Second stage sampling involved randomly selecting 12 compounds. All consenting occupants of the compound over the age of 6 months were eligible for inclusion in the study.

The survey was conducted in July 2009, at the end of the long rainy season. At each of the households, informed written consent was sought. The head of household was interviewed to assess household wealth indices and structure and individuals were questioned about malaria control behaviours. A finger-prick blood sample was taken from all consenting household members above the age of 6 months to measure parasite prevalence using PfHRP2 RDT (Paracheck Pf®, Orchid Biomedical Systems, Goa, India) and haemoglobin levels (HemoCue®, Angelholm, Sweden). Blood was also spotted onto filter paper (3MM Whatman®, Maidstone, UK) for subsequent molecular and serological analysis. Individuals with a positive RDT result were treated for malaria at the home according to national guidelines with artemether-lumefantrine (Coartem®, Novartis) or referred to a health facility if suspected to be pregnant or allergic to Coartem®. Participants found to be anaemic were provided haematinics according to national guidelines at the time of the survey.

Ethics, consent and permissions

This study was approved by the ethical committees of the London School of Hygiene and Tropical Medicine and the Kenya Medical Research Institute (#SSC 1802). Approval was also provided by the Division of Malaria Control, Ministry of Public Health and Sanitation. Prior to the surveys, meetings were held with all district administrative representatives, chiefs and district health teams to inform them of the study and acquire local approval. Community meetings were held at each of the EAs to explain the methods of study to potential participants. Household heads were approached and the study explained to members of the home. Individual informed consent was sought from all residents of the compound above the age of 6 months by signature or thumbprint accompanied by the signature of an independent witness. Consent for children under the age of 18 was provided by a parent/guardian and children between 14 and 17 years also provided written assent by signature or thumbprint accompanied by the signature of an independent witness. As defined in the Kenya national guidelines, participants below 18 years of age who were pregnant, married, or a parent were considered ‘mature minors’ and consented for themselves [22].

Laboratory procedures: ELISA

Antibody responses to P. falciparum were assessed by ELISA as previously described [23]. Briefly, recombinant protein P. falciparum MSP-119 was coated on plates at a concentration of 0.5 µg/mL in coating buffer and incubated at 4 °C overnight. After blocking with 1 % (w/v) skimmed milk solution, the plates were washed, and samples were added in duplicate at a final dilution of 1:1000, with a pool of hyper immune serum titrated on each plate. Plates were incubated overnight at 4 °C and 50 µl of HRP-conjugated rabbit anti Human IgG (DAKO, #P0214) were added into each well and incubated. After a further series of washes, substrate solution (OPD, Sigma #P8287, in PBS) was added and the reaction was allowed to develop for 15–20 min before addition of stopping solution (2 M H2SO4). Raw optical density (OD) measurements were averaged and normalized against the positive control samples on each plate. The cut-off value of these assays to define seropositivity was determined using a mixture model, which considers those individuals with OD values greater than the cut-off determined by the model as being seropositive. Age adjusted SCR were calculated by fitting a simple reversible catalytic model using the seropositivity data to determine the rate at which individuals convert from seronegative to seropositive by year of age. The seroreversion rate (SRR) was fixed at a reversion rate of 4.057 for both population and cluster specific calculations. The SRR used was obtained according to estimates from previous studies, and was fixed to ensure robust estimates of SCR, particularly with the smaller sample sizes for the cluster-level estimates [14, 23, 24].

Laboratory procedures: PCR

The presence of a current P. falciparum infection was assessed using nested PCR (nPCR). Blood-spotted filter papers were punched with a sterile hole punch and DNA was extracted using a Chelex-saponin method as described previously [25]. In brief, filter papers were incubated in a 0.5 % saponin-PBS solution overnight. Filter paper spots were washed once in a 1× PBS solution and DNA was eluted by boiling samples in a 6 % Chelex DNA/RNAase free solution. Extracted DNA was then added to two separate nPCR reactions targeting the ribosomal 18S [26] and a modified version targeting mitochondrial Cytochrome B region [27]. Gel electrophoresis was performed and samples were visualized on an ethidium bromide stained 2 % agarose gel. Samples were considered to be positive if they were positive in either assay.

Statistical analysis

All statistical analysis was conducted in STATA (v 12.0, StataCorps, Texas, USA) using the survey (svy) command to account for the two-stage sampling design (EA and compound) and stratifying by district. Prevalence and means and the corresponding exact 95 % confidence interval (CI) were calculated assuming a binomial and normal distribution, respectively. Tests of significance were assessed using the Spearman’s and Chi square test as appropriate. Socio-economic status (SES) was generated using a principal component analysis (PCA) using household level wealth indicators collected as part of the questionnaire [28]. Indicators included education level attained by the head of household, house construction including wall, floor and roof type, as well as ownership of items such as animals, televisions, mobile phones, vehicles, and bicycles. To assess the association between the three outcome measures in this study, RDT, PCR and seroprevalence, logistic regression was conducting using the svy command as described above. Univariate analysis was conducted to determine the association with all potential risk factors. Next, a multivariable analysis was conducted in a backwards step-wise fashion retaining all variables significant at the 0.05 level. Variables included in the analysis were anaemia status (haemoglobin <11 g/dl), recent travel, the type of eaves present in the house, as well as the use of mosquito control measures [insecticide-treated nets (ITNs), indoor residual spraying (IRS)]. Finally, elevation was included as an ordinal variable with cut-offs defined using quartiles. The optimum model was determined using a likelihood ratio test comparing the saturated to the reduced model.


In total, 3566 people were sampled from 46 EAs with a mean of 78 (range 70–96) people sampled per EA (Table 1). Forty per cent (95 % CI 38.3–41.8, range 27.5–54.4) of the population sampled were ≥20 years of age, whilst a low proportion of samples came from those aged 15–20 years (7.4 %, 95 % CI 6.5–8.5 %, range 0–27.1 %). 56.3 % (95 % CI 54.7–57.9, range 47.3–68.0) were female. Use of bed nets was the most common form of malaria control with 60.3 % (95 % CI 58.7–61.9, range 12.3–91.7) of the population reporting having slept under a net the previous night. 24.0 % (95 % CI 19.6–29.1, range 0–70.4) of the population reported that they did not use bed nets nor had their house been sprayed in the past 12 months.

Table 1 Demographic data of all surveyed households

Malaria infection by RDT was low overall with a mean prevalence of 3.0 % (95 % CI 2.2–4.2 %) but this ranged by EA from 0 to 13.1 % (Table 2). The malaria prevalence by PCR was over twice that of RDT with 8.5 % (95 % CI 7.0–10.4 %) being positive and 64.4 % (95 % CI 59.4–69.4 %) of PCR infections were sub-patent (i.e., PCR positive but RDT negative). The parasite prevalence by PCR was also highly heterogeneous with clusters ranging from 0 to 14.8 %. Exposure as assessed by antibody prevalence to malaria MSP antigen was 36.8 % (95 % CI 33.9–39.8 %) and varied by EA from 18.6 to 65.8 % (Table 2). Also, seropositivity in children <5 years old varied from 0 to 44 % across EAs suggesting heterogeneity in recent exposure across the study area. Mean SCR was 0.038 (95 % CI 0.035–0.040). There was a significant association between the rank of RDT and PCR prevalence at the EA level (Spearman’s p <0.001), but no association between SCR or seroprevalence and RDT (p = 0.185; p = 0.223) and PCR (p = 0.096; p = 0.114), respectively.

Table 2 Overall estimates of current malaria infection and exposure, as well as haemoglobin levels

Children aged 5–10 years had the highest malaria prevalence by both RDT (6.1 %, 95 % CI 4.2–8.0 %) and PCR (13.1 %, 95 % CI 10.4–15.7 %) (Table 3). However, older age groups were found to have the highest proportion of sub-patent infections; 78.1 % of PCR-confirmed infections in 15–20 year olds and 73.3 % of infections in those >20 years were sub-patent compared to 51.3 % in children <5 years (chi p = 0.015). Older children also reported the lowest bed net use; 55.6 % (95 % CI 51.0–60.2 %) and 56.5 % (95 % CI 50.6–62.5 %) in the 10–15 and 15–20 years age groups, respectively (Table 3).

Table 3 Malaria outcomes and factors commonly associated with malaria stratified by age group

Of the 46 EAs included in the survey, 15 had no RDT-positive infections detected (Fig. 2a). In these clusters with no evidence of infection by RDT, the mean PCR prevalence was 5.0 % and this ranged between 0 and 12.2 % (Fig. 2b). Similarly, despite no RDT-positive individuals present, 32.5 % of individuals were positive for MSP antibodies and this ranged from 18.6 to 65.8 % (Fig. 3). In the 15 RDT-negative clusters, when seroprevalence estimates were restricted to those under 5 years of age, a proxy for recent transmission, 14.3 % of children were seropositive and this ranged from 0 to 43.7 %.

Fig. 2
figure 2

Map of EAs by a RDT prevalence, b PCR prevalence

Fig. 3
figure 3

Map of EAs by seroprevalence

Variation according to EA altitude was observed for serological and parasitological outcomes. There was a large range in SCR across EAs (range: 0.013–0.128) and showed a non-significant negative trend with SCR decreasing by 0.0002 (95 % CI −0.0004 to 0.00001) with every 1 m increase in elevation. SCR was also highly variable by EAs with a similar mean elevation (Fig. 4a). For one EA in particular, SCR was significantly higher than those of the same altitude range. However, when stratifying the SCR by altitude band, those residing at an elevation between 1550 and 1665 m did show a significantly lower SCR compared to those residing at the lower elevations (Fig. 5a). Despite the overall RDT (Fig. 4b) and PCR (Fig. 4c) prevalence being low, no linear trend (increase of ~0 per increase in 1 m of elevation; RDT, p = 0.99; PCR, p = 0.32) was observed between EAs and elevation.

Fig. 4
figure 4

Scatter plots of malaria estimates per cluster by elevation, a seroconversion rate, b RDT prevalence, c PCR prevalence. Plots include 95 % CI

Fig. 5
figure 5

a Seroconversion curves per altitude band, b seroconversion curves per self-reported mosquito control

Next, seroprevalence curves were calculated based on the different reported mosquito control interventions. As would be expected, the group reporting having both received IRS and having slept under a bed net the previous night showed evidence of lower exposure compared to those reporting using a single intervention (Fig. 5b; data shown in Additional file 1). Interestingly, the exposure in groups having reported using a single mosquito control intervention, either bed nets or IRS, showed no difference compared to the group that reported using no malaria control interventions (Fig. 5b).

Factors associated with malaria infection by RDT and PCR, and malaria exposure, defined as MSP seropositivity, were also assessed. The unadjusted analysis for RDT positivity suggested that older age was associated with reduced odds of infection whereas reporting use of IRS and residing in a house with open eaves increased the odds of being RDT positive (Table 4). Next, an adjusted analysis for RDT positivity was conducted (Table 5). Children between 5 and 10 years of age showed the highest odds (AOR 1.81; 95 % CI 1.16–2.81; p = 0.009) of being infected compared to those under 5 years, whilst adults over 20 years were the least likely to be infected (AOR 0.27; 95 % CI 0.12–0.62; p = 0.003). Also, residing in a house with open eaves was associated with five times the odds of RDT infection (AOR 5.05; 95 % CI 1.6–16.0; p = 0.007). Use of mosquito control was also associated with having a patent infection with only those reporting use of IRS only being significant (AOR 2.61; 95 % CI 1.01–6.78; p = 0.48).

Table 4 Univariable logistic regression analysis of factors associated with infection and exposure to Plasmodium falciparum
Table 5 Multivariate logistic regression analysis of factors associated with infection and exposure to Plasmodium falciparum

In the unadjusted analysis using PCR positivity as the response variable, results were similar to those of RDT positivity. There were increased odds of infection in older children and in those residing in households reporting use of IRS only, whereas females were less likely to be infected (Table 4). In the adjusted analysis, PCR infections were greater in children aged 5–10 years compared to children <5 years (AOR 1.73; 95 % CI 1.22–2.45). Wealthy households also exhibited lower odds of PCR infection compared to the poorest households although not all SES categories were statistically significant (Table 5). Finally, there was borderline evidence (p = 0.051) to suggest that reporting use of both IRS and bed nets had greater odds of PCR infection than those using no interventions (AOR 1.67; 95 % CI 1.00–2.79). Those who reported using only IRS had over twice the odds of being PCR positive (AOR 2.13; 95 % CI 1.21–3.75) compared to those with no vector control.

As expected for antibodies to P. falciparum MSP-119, age was significantly associated with seropositivity (Table 4). As seen in Fig. 5a, a significant difference in seropositivity was observed only between EAs at the highest altitude compared to the lowest (OR 0.68, 95 % CI 0.52–0.77). Females also had increased odds of being seropositive (OR 1.41, 95 % CI 1.21–1.64). In the adjusted analysis those greater than 20 years had 8.18 times the odds of being seropositive compared to children under five (95 % CI 6.51–10.27; p < 0.001). Also, being anaemic was associated with increased odds of being seropositive (AOR 1.26; 95 % CI 1.02–1.56; p = 0.034). Both elevation and SES were associated with decreased odds of seropositivity (Table 5). Those residing at the highest altitude had 35 % reduction in odds of being seropositive (AOR 0.63; 95 % CI 0.46–0.86; p = 0.005) compared to those living in the lowest elevation band. Similarly, those in the highest SES quintile had an AOR of 0.60 (95 % CI 0.52–0.92; p = 0.013) compared to the poorest quintile.


In many areas, as malaria control interventions are scaled up, transmission is being reduced, resulting in an epidemiological transition with changes in disease burden [1]. Factors associated with these transitions are likely multifaceted and due partly to both curative (increased availability and use of RDT and artemisinin-based combination therapy) and preventative measures (increased coverage of ITNs and IRS) [2931]. As transmission decreases, surveys such as this one conducted in the western Kenyan highlands are important to understand the current local malaria epidemiology, describe the heterogeneity of transmission and identify areas where transmission persists to allow better targeting of control interventions. In this cross-sectional survey, three different but complimentary tools to assess malaria transmission were assessed: RDTs to determine those P. falciparum infections that would be targeted for treatment, PCR to identify current infections including those of low level parasitaemia and serology to describe historic exposure. The results here describe a large range in malaria exposure within this area of nominally low transmission and that the more sensitive diagnostics, using PCR to detect parasite DNA and ELISA to detect antibodies to malaria, provide added value in terms of understanding the epidemiology in this setting and determining risk factors for malaria.

RDTs are useful in a clinical setting and can provide a crude metric for malaria prevalence. However, not unexpectedly, in this study a large proportion of the infections that were detected using the more sensitive PCR metric were missed by RDT. The presence of a large reservoir of sub-patent infections is common in low transmission settings where parasite densities tend to be below the detection threshold of RDTs or microscopy [10]. Therefore, the more sensitive PCR metric provides an accurate estimate of the parasite reservoir in the community, the identification of which is essential when considering malaria elimination [32]. The expense of the equipment and reagents and time taken to conduct current versions of molecular assays limit use as a routine diagnostic test [33], however these tools can identify which sections of the population harbour sub-patent infections and therefore could be targeted for community-level control. For example, in this study the highest levels of sub-patent infection were in those over the age of 15 years.

Seroprevalence to the P. falciparum MSP antigen provided insight into malaria exposure levels in the study area. SCR has been associated with the entomological inoculation rate (EIR), which provides a measure of transmission intensity [23]. The wide range of SCR as well as the range of seroprevalence in children <5 years of age suggests that malaria exposure is highly variable in this area. As a measure that integrates exposure over time and reflects cumulative exposure rather than a single current infection, serology can provide a more robust picture of the malaria transmission dynamics in an area. In this setting, in the EAs with no evidence of infections by RDT, serological and molecular tools enabled a more complete understanding of the ongoing transmission and allowed for an examination of risk factors. Serological outcomes can supplement metrics of current infection and be used to identify risk factors where transmission is low [34, 35].

In this population, consistent with other findings, age and wealth indicators were both associated with malaria infection by all metrics [36]. Children between 5 and 10 years of age were more likely to be positive by both RDT and PCR compared to children under 5 years of age, whilst those over 20 years had reduced odds of RDT-confirmed infection. Those of higher SES were less likely to be infected with or show historical exposure to malaria. The presence of open eaves on households was also associated with being RDT positive, as would be expected due to increased house entry of the vector [37, 38]. House screening has been shown to reduce mosquito entry and reduce the odds of malaria infection [3941].

Interestingly, altitude of residence of survey participants was not associated with either measure of current infection in the adjusted analysis and only with malaria exposure, determined by serology, at the highest altitudes. This lack of association may be due to the low RDT and PCR prevalence in this population or due to the pre-selected narrow altitude range. However, this suggests that the association with altitude and current infection may be more useful at a larger spatial scale [42] or that in this setting, measures of current infection may be less stable on such a short time scale but is consistent with altitude over longer time periods. Also, recent travel was not associated with any malaria metric even though travel is consistently seen as a risk factor for malaria [43]. However, travel, as a risk factor for malaria, will be dependent on who is travelling and their destination in terms of changes in risk. Previous studies have shown those with higher SES are more likely to travel and may have a lower overall risk of malaria infection through increased knowledge and use of protective measures. Also, it has been found that the majority of people in this population travel to Nairobi, the capital city, which has a low or negligible risk of malaria compared to the Kisii and Rachuonyo [44]. Therefore, the lack of association with travel is not surprising.

Regression analyses suggested that those who had received IRS in the past 12 months had an increased risk of being malaria positive at the time of the survey. Although this is at first counter-intuitive, this finding is likely confounded by the fact that areas with higher perceived risk tend to be the ones more like to be targeted and receptive to the use of mosquito control [45]. In 2009 areas considered to be of higher risk in Kenya were targeted for the IRS campaign. Also, that year the IRS campaign was conducted after onset of the rains; at the time of the survey the current campaign was nearing completion. The RDT and PCR results may therefore have not measured any benefit of the campaign that year.

It was observed that those who reported using both ITN and IRS resided in a cluster with a significantly lower SCR than those using just a single intervention. This suggests that combining interventions may be more efficient at reducing exposure and driving transmission to even lower levels, as demonstrated in modelling studies [4648]. Data from studies in Bioko Island, lowland Kenya and Tanzania indicated combining ITN and IRS resulted in lower prevalence and incidence of malaria than those who received only nets [19, 4951]. However, other trials and observational studies in Africa indicate little or no added benefit of combining interventions [5255]. As each intervention is scaled up within Kenya and across the continent, it is increasingly likely that people will be protected by both ITN and IRS [56] and therefore the synergistic effect of the combined intervention coverage should be further explored [54].

One limitation of the serology component of this study was the use of only one antigen (which was all that was available at the time) to assess anti-malarial antibody responses. Increasing the number of antigens would most likely lead to marginal increases in the overall seroprevalence estimates due to heterogeneous immunity in the population. However, whilst this means that the serological outcomes presented here can be assumed to be underestimates, responses to non-variant antigens such as MSP-119 with a comparatively long antibody half-life [57] should be considered reasonably robust [58]. Furthermore, it is acknowledged that the small sample sizes within each cluster affects the precision of the estimated cluster-level SCR and are highlighted in the width of the reported confidence intervals. Calculations of SCR would be more robust with standardized age ranges per cluster but as the household survey was designed and powered to estimate parasite rate, and given that the sampling framework included all ages and the SRR was fixed, the bias is minimized [24].

In this study, EAs were sampled from two districts: Kisii is classified as having low and epidemic-prone malaria transmission whereas Rachuonyo has low and stable transmission. However, analysis of malaria burden determined by all metrics at the EA level showed no clear differences, with those with the highest burden being equally in both districts. The cluster with the highest SCR was in fact located in Kisii, the district with lower overall reported levels of malaria burden (although the low sample sizes used to estimate SCR on the EA level are acknowledged). This suggests that allocating resources at the district level may not be ideal; pockets of high burden within the overall low transmission area would be missed and may seed transmission to neighbouring areas [59]. Although it is logistically more difficult to deploy malaria control interventions at a more refined spatial scale, such efforts may prove to be more efficient as interventions could be more precisely targeted and prove to be more cost-effective than blanket treatment. Therefore, strong health systems and surveillance based on sensitive diagnostic tools, as well as the capacity to analyse the data collected, will be necessary for informed decisions to be made at a time scale that is relevant for malaria control.


Ultimately, in the highlands in western Kenya, malaria transmission is generally low, but highly heterogeneous and difficult to measure accurately using routine diagnostic tools. This study found a large range in malaria burden at the EA level and a large population of sub-patent infections. This suggests that for malaria surveillance and monitoring, more sensitive metrics such as molecular or serological methods may complement current diagnostic tools in order to accurately assess malaria burden in the population, determine reservoirs of infection, identify risk factors and, therefore, better target appropriate interventions.