Author Correction to: BMC Biol 18, 102 (2021)
https://doi.org/10.1186/s12915-020-00836-x
Following publication of the original article [1], it has been brought to the authors’ attention that after 16S sequencing of the v1-8 region, the bacterium which we originally believed to be F. prausnitzii (a butyrate-producer) in our penultimate figure, Fig. 5k and Additional file 1: Figure S5B, was in fact L. plantarum (a lactate-producer). This does not affect the other data, especially the animal work, nor does it alter the final conclusions of this manuscript.
The correct Figs. 5 and S5 and their caption have been included below and the fully corrected version of Additional file 1 is attached to this Author Correction, and the original article [1] has been corrected.
Figure S5. Cell survival analysis of RT112 bladder tumour cells treated with SCFAs and bacterial supernatants. (A) Inhibition of cell viability of RT112 cells single SCFA and combined SCFAs mixture in a time-dependent manner (N=3). The combined SCFAs denote the mixtures of 10 mM butyrate, 10 mM propionate, 10 mM butyrate for the left-hand graph and the mixtures of 10 mM butyrate, 5 mM propionate, 1.7 mM butyrate for the right-hand graph. (B) Reduced cell survival of RT112 cells by bacterial supernatants at day 3 (N=1). BA+LP denotes the cross-feeding of B. acidifaciens and L. plantarum, while Bif+LP denotes the cross-feeding of Bifidobacterium and L. plantarum. *P<0.05; **P<0.01; ***P<0.001.
Furthermore, some sentences in the original article’s main text need to be corrected. The affected text has been highlighted in bold typeface, and the original article [1] has been corrected.
Previous version
Results
To validate the anti-tumoural effects of B. acidifaciens, we treated the bladder tumour cells with bacterial supernatants of B. acidifaciens and its cross-feeding with F. prausnitzii, and compared their effects with Bifidobacterium (acetate-producer) and F. prausnitzii (butyrate-producer). Bacterial supernatants of B. acidifaciens and its cross-feeding with F. prausnitzii significantly increased cytotoxicity of bladder tumour cells compared to the other supernatants in day 2 (Figure 5K) and in day 3 (Additional file 1: Figure S5B).
Discussion
In this study, we revealed that bacterial supernatant from B. acidifaciens and its cross-feeding with F. prausnitzii caused significantly higher levels of cytotoxicity compared to the other supernatants (Figure 5K and Additional file 1: Figure S5B). This result supports our finding that B. acidifaciens may drive the radiosensitising effect. Moreover, B. acidifaciens in vitro has a greater effect on cell kill than F. prausnitzii (butyrate-producer; p<0.001), implying that metabolites other than butyrate may be involved in its effect.
Methods
All bacterial strains were obtained from DSMZ-German collection of microorganisms. Three strains of bacteria, namely B. acidifaciens (BA; DSM 15896), Bifidobacterium animalis (Bif; DSM10140), F. prausnitzii (FP; DSM17677), and two cross-feeding combinations (BA+FP and Bif+FP) were cultured in Gifu Anaerobic Broth, Modified (GAM; Nissui Pharmaceutical, Japan).
Corrected version
Results
To validate the anti-tumoural effects of B. acidifaciens, we treated the bladder tumour cells with bacterial supernatants of B. acidifaciens and its cross-feeding with L. plantarum, and compared their effects with Bifidobacterium (acetate-producer) and L. plantarum (lactate-producer). Bacterial supernatants of B. acidifaciens and its cross-feeding with L. plantarum significantly increased cytotoxicity of bladder tumour cells compared to the other supernatants in day 2 (Figure 5K) and in day 3 (Additional file 1: Figure S5B).
Discussion
In this study, we revealed that bacterial supernatant from B. acidifaciens and its cross-feeding with L. plantarum caused significantly higher levels of cytotoxicity compared to the other supernatants (Figure 5K and Additional file 1: Figure S5B). This result supports our finding that B. acidifaciens may drive the radiosensitising effect. Moreover, B. acidifaciens in vitro has a greater effect on cell kill than L. plantarum (lactate-producer; p<0.001), implying that metabolites other than lactate may be involved in its effect.
Methods
All bacterial strains were obtained from DSMZ-German collection of microorganisms. Three strains of bacteria, namely B. acidifaciens (BA; DSM 15896), Bifidobacterium animalis (Bif; DSM10140), L. plantarum (LP), and two cross-feeding combinations (BA+LP and Bif+LP) were cultured in Gifu Anaerobic Broth, Modified (GAM; Nissui Pharmaceutical, Japan).
Reference
Then CK, Paillas S, Wang X, Hampson A, Kiltie AE. Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation. BMC Biol. 2020;18(1):102 https://doi.org/10.1186/s12915-020-00836-x.
Author information
Authors and Affiliations
Corresponding author
Supplementary Information
Additional file 1: Figure S1.
Similar bacterial components in the faecal and caecal microbiomes. Figure S2. Faecal butyrate levels and time taken for tumours to reach 50 mm3. Figure S3. Differences in composition of the gut microbiome when tumours reached 350 mm3. Figure S4. Individual mouse tumour growth curves. Figure S5. Cell survival analysis of RT112 bladder tumour cells treated with SCFAs and bacterial supernatants. Figure S6. Correlation of time to culling with B. acidifaciens or Parabacteroides genus abundance different groups. Figure S7. Effect of cage location of mice on relative abundance of B. acidifaciens and Parabacteroides genus. Table S1. Rodent diets used in the study with varying levels of cellulose or inulin per 4000 kcal. Table S2. Details mouse diets, cages, B. acidifaciens relative abundance and time of culling.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Then, C.K., Paillas, S., Wang, X. et al. Author Correction to: Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation. BMC Biol 19, 139 (2021). https://doi.org/10.1186/s12915-021-01066-5
Published:
DOI: https://doi.org/10.1186/s12915-021-01066-5