Abstract
Background
Hepatitis B virus (HBV) and Hepatitis C Virus (HCV) infections are global issues that disproportionately affect developing countries. Pregnancy-related HBV and HCV infections are associated with a high risk of vertical transmission and complications for the mother as well as the newborn. Therefore, this study aims to determine the seroprevalence of hepatitis B virus and hepatitis C virus infection among pregnant women attending antenatal care at Guhala Primary Hospital, Northwestern Ethiopia.
Methods
A hospital-based retrospective study was conducted from July to September 2022 on HBV and HCV registered books from September 1, 2017, to August 30, 2019, for a year. The presence of HBsAg and anti-HCV in serum was detected using the One Step Cassette Style HBsAg and anti-HCV antibody test kit. Data were analyzed using SPSS version 26 software.
Results
In this study, a total of 2252 participants for HBsAg and 538 participants for ant-HCV rapid tests of records in the laboratory logbook were included. The mean age of the study participants was 25.6years (± 5.8SD). The overall prevalence of HBsAg and anti-HCV was 6.0% (134/2252) and 2.4% (13/538), respectively. There were 0.4% (2/538) coinfection results between HBV and HCV among pregnant women.
Conclusion and recommendation
In this study, intermediate seroprevalence of HBV and HCV infection was detected among pregnant women attending antenatal care. The Hepatitis B virus was predominantly higher among pregnant women aged between 25 and 34 years. To manage and stop the potential vertical transmission of these viral agents during the early stages of pregnancy, routine prenatal testing for HBV and HCV infections should be taken into consideration.
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Introduction
Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infection is an emerging worldwide public health concern affecting millions of people each year [1,2,3]. HBV and HCV are the etiology of HBV and HCV infection. These infections can cause both acute and chronic hepatitis, as well as possibly induce cirrhosis, liver cancer, or even death in those who are affected [3,4,5].
Globally, HBV and HCV cause chronic infections in 254 million and 50 million persons worldwide, respectively, and these diseases result in 1.1 million and 242, 000 deaths annually, respectively [6]. In developing countries like Asia and Africa, HBV and HCV infections are very common [7]. The World Health Organization reported that there were 1.2 million and 1.0 new cases of chronic HBV and HCV infection worldwide in 2022, respectively [6]. According to WHO estimates between 3 and 4 million people are acquired the disease annually, with the majority of cases occurring in Africa [8]. Other evidence also showed that an estimated 71.1 million people are infected with HCV worldwide [3, 9]. Despite being highly contagious, HBV and HCV are still underdiagnosed and underreported in the majority of African countries [10].
Both HBV and HCV have the potential to be widespread and impact a variety of populations, such as those with HIV, healthcare professionals, the general population of blood donors, pregnant mothers, and their children [11,12,13,14,15]. Numerous studies show that the prevalence of HBV and HCV in pregnant women is now seriously associated with public health [14, 16, 17]. Mother-to-child transmission of HBV, which might be through intrauterine transmission is a common event and causes chronic infection of the virus [18]. The seroprevalence of HBV among pregnant women in Ethiopia ranges from 4.5 to 7.9%, which is indicative of an intermediate degree of endemicity for the virus [19,20,21,22,23]. The seroprevalence of HCV, which ranges from 0.26 to 8.07%, was shown to be lower than that of HBV in the majority of Ethiopian studies [17, 23,24,25].
Childbearing women can potentially transmit HBV and HCV to their children’s. They transmit an infection to newborns usually during birth or soon after birth following close contact. There is a higher likelihood of vertical transmission of infection from mothers to offspring in 4.6% and 1.6% of babies delivered to pregnant women with HBV and HCV infections, respectively [26]. Newborns who are exposed to HBV will have almost 85–90% risk of developing chronic liver diseases [27].
Preterm delivery, placental separation, vaginal bleeding, early rupture of the membranes, and mortality are among the many issues that can arise from maternal infection with HBV and HCV during pregnancy [28, 29]. A significant risk of neonatal hepatitis is also linked to it, and this can result in liver cirrhosis and hepatocellular cancer in young adulthood [30, 31]. Generally, in the health system of Ethiopia, the health burden due to viral hepatitis is still less [32, 33]. Even though viral hepatitis screening is advised during routine antenatal care (ANC), routine antenatal screening of pregnant women is not common and obligatory in Ethiopia [34]. Recent research showed that both the general population and healthcare professionals reported having little knowledge of the hepatitis virus [25, 32]. Therefore, this study aimed to determine the seroprevalence of HBV and HCV among pregnant women for the last three years using a laboratory-recorded book to fill the current epidemiologic gap in the area.
Materials and methods
Study design, area, and period
A hospital-based retrospective study was conducted among pregnant women from HBV and HCV registered books at Guhala Primary Hospital, Northwestern Ethiopia, from September 1, 2017, to August 30, 2019. Guhala is the capital town of East Belesa, located northwest of Addis Ababa (the capital city of Ethiopia), which is 718 km away. Guhala town has an estimated total population of 148,758, of whom 75,732 were males and 73,026 were females. The town has only one public primary hospital, one health center, one health post, and four private health facilities. Guhala Primary Hospital is the only public hospital. Today, the hospital is providing health services to a total population of about 148,758. The data collection period was from July to September 2022.
Source population, study population, sample size, and sampling technique
The source populations were all pregnant mothers who were attending ANC in Guhala Primary Hospital in northwestern Ethiopia. The study population consisted of all pregnant mothers who had requested Hepatitis B surface Antigen (HBsAg) and anti-HCV screening from the respective ANC follow-up and gave blood for HBsAg and anti-HCV screening and recorded in the Laboratory logbook at the Guhala Primary Hospital from September 1, 2017, to August 30, 2019. The record of data HBsAg and anti-HCV statuses, years, and ages were included in this study. Patient information with incomplete demographic characteristics, and test results in the log book was excluded.
Data collection tools
We collected data from all pregnant women who screened for HBsAg and anti-HCV from serology registration logbooks using a structured checklist. Client information, including age, laboratory results, and year of diagnosis was obtained from the serology laboratory registration logbooks.
Laboratory methods
The hepatitis B surface antigen (HBsAg) and anti-HCV antibody test were requested as part of the antenatal care panel. A 5 ml of venous blood was collected from each pregnant woman. Then, the serum was separated by centrifugation at 5000 rpm for 15 min and tested for the presence of HBsAg and ant-HCV antibodies using a one-step HBsAg test strip (Nantong Diagnosis Technology Co., Ltd., China) and a one-step HCV test strip (Nantong Diagnosis Technology Co., Ltd., China), respectively, by following the instructions of the manufacturer. The sensitivity and specificity of rapid test kits of HBsAg and one-step HCV test strips were 99.1% and 99.6%, respectively.
Data quality control
We used different methods to ensure that the collected data had the required quality. For instance, we used a structured data collection checklist and regular communication with the hospital staff working at the serology laboratory. Finally, we checked the collected data, cleaned it manually, and entered SPSS version 26 for analysis.
Data processing and analysis
All participants’ information and laboratory data were entered and then analyzed using SPSS version 26 (IBM Corp., Armonk, NY). Descriptive analysis was used to describe and calculate the frequencies and percentages of variables.
Results
Socio-demographic characteristics of study participants
Over the five years (2017–2019), a total of 2,252 serologically tested pregnant women were included. The age of patients’ ranges from fifteen to sixty-five years and the mean age of the patients were 25.6 years (± 5.8 SD). Most of the samples were tested in 2019 (37.3%), followed by 2018 (33.5%), and 2017 (29.3%). Most of the samples came from pregnant women between the ages of 15 and 24 years (45.6%), followed by those the age between of 25–34 (44.2%) and those the ages of 35–44 years (9.6%). On the other hand, over the five years (2017–2019), a total of 538 samples collected from pregnant women were tested for anti-HCV at the Guhala Primary Hospital serology laboratory. Most of them, 45.3% (243) were between the ages of 15 and 24, followed by those the age between of 25–34 (44.3%) (Table 1).
Sero-prevalence of HBV and HCV among pregnant women
The overall prevalence of HBsAg and anti-HCV were 6% (134/2252) (95% CI = 5–7%) and 2.4% (13/538) (95% CI = 1.3–3.7%), respectively. Hepatitis B virus was predominantly higher among pregnant women aged between 25 and 34 years old 7.13% (71/995) followed by individuals aged between 15 and 24 years old 5.0% (51/1028). On the other hand, the prevalence of hepatitis C virus was high frequency among pregnant women in the age group between 35 and 44 years old 6% (3/51). There were 0.4% (2/538) coinfection results between HBV and HCV among pregnant women. Moreover, a high percentage of HBsAg and anti-HCV were seen in the year 2018 and 2019, respectively (Table 2).
Discussion
The seroprevalence of HBsAg in the current study was 6% (134/2252) 95% CI = 5–7%), and this infectivity rate can be considered as a highly endemic scenario for the virus [35]. The seroprevalence of HBsAg in the present study was comparable with other similar studies conducted in West Hararghe, Ethiopia (6.1%) [36], Borena Zone (5.7%) [37], Nekemte (5.8%) [38], Bishoftu (6.5%) [39], and Dilla, Ethiopia (5.1%). However this report is lower than the study conducted in Debre Markos, Ethiopia (8.3%) [40], Borumeda, Ethiopia (8.1%) [41], Adigrat General Hospital, Ethiopia (9.2%) [42] Jijiga, Ethiopia 8.5% [43], Southern, Ethiopia (10.9%) [44] and (7.8%) [45], Gedeo zone (9.2%) [46], Gambella (7.9%) [22], the Republic of South Sudan (11%) [47] and (8.5%) [48], Tanzania, (8.03%) [49], Uganda (11.8%), Gambia (9.2%) [50], Ghana (7.7%) [51], Cameroon (10.78%) [52], and Nigerian (14.1%) [53].
On the contrary, the result of this study was relatively high when compared to the result reported in Gondar, Ethiopia (4.6%) [54], Felegehiwot referral hospitals, Ethiopia (4.7%) [55], Bahir Dar, Ethiopia (3.9%) [13] and (3.8%) [56], Gandhi memorial hospital, Ethiopia (2.3%) [57], Addis Ababa, Ethiopia (4.5%) [58], Shone (4.9%) [59], Agena health center in the Gurage zone (4.1%) [60], Ambo town, Ethiopia (4.99%) [61], Southern, Ethiopia (4.5%) [23], Rwanda (3.1%) [62], Uganda (2.1%) [63], and Pakistan (3.7%) [64]. The variation may result from differences in the study period, sample size, population demographics, and clinical conditions of study participants across different locations, and cultural practices and methods utilized for infection prevention and unsafe behavior practices. When compared to other research conducted outside of the African continent, the study’s findings were higher than the study conducted in Pakistan (2.78%) [65], Turkey (2.1%) [66], and India (1.6%) [15]. The variation may be the result of the routine immunization and screening programs in those countries.
In the present study, we found that the majority (7.1%) of the study participants infected by hepatitis B were in the age group 25–34 years. This finding is similar to a study in Adigrat, Ethiopia where the majority of the study participants infected by hepatitis B were in the age group 25–34 years [42].
In this study, the seroprevalence of HBV among pregnant mothers was higher than that of HCV (6% versus 2.4%). This is similar to studies conducted in Ethiopia (8.1% versus 3.2%) [41], ( 4.6% versus 1.6%) [54], and Pakistan (3.7% versus 2.1%) [64]. In contrast to this a study conducted in West Oromia, Ethiopia, showed a higher HCV rate (8.07%) than HBV rate (2.4%) among pregnant women [17]. Another similar study in the Democratic Republic of Congo also showed a slightly higher HCV rate of infectivity (4.8%) than HBV (3.9%) [67]. In Egypt, HCV was found to be the more common infection than HBV, despite the extremely low prevalence [14]. This dominance of HCV over HBV in other studies might be associated with a non-detection ability of HBsAg due to mutation on the surface antigen gene [68].
In the present study, we found 2.4% (95% CI = 1.3–3.7%) of the overall seroprevalence of HCV Among pregnant women screened for HCV infection in Guhala Primary Hospital. This finding is consistent with the study conducted in Gondar Ethiopia (1.6%) [54], southwest, Ethiopia (2.3%) [69] Southern Ethiopia 1.83% [23], and systematic review and meta-analysis in Ethiopia (1.83%) [24]. However, this finding is lower than the study conducted in the East Wollega zone (8.07%) [17] and Ghana (7.7%) [70]. On the other hand, our study is higher than the study conducted in Bahir Dar, Ethiopia, (0.62%) [71] and (0.26%) [25], and Dessie, Ethiopia (0.7%) [72].
In the present study, there was a slight connectivity rate between the two viruses among pregnant women. This study is concordant with the study conducted in Ethiopia [54], Nigeria [73], and Ghana [70], where, a similar scenario in which the presence of HBV and HCV connectivity was documented. Besides, this study is discordant with the study conducted in Ethiopia [25, 41], Nigeria [74, 75], and Pakistan [64], Where, the absence of HBV and HCV connectivity was documented. A study carried out in China clarified HCV spontaneous clearance among HBV/HCV-co-infected study subjects, and it also demonstrated that female gender, concentration of HBV DNA, and genotype are associated with enhanced spontaneous clearance of HCV [76].
Limitations of the study
The study has drawbacks related to missing data and an insufficient document-keeping system since it relied on secondary sources. Another drawback is that only the detection of HBsAg and anti-HCV has been possible in laboratory testing and enzyme linked immunosorbant assay and the Nucleic Acid Amplification Technique was not employed.
Conclusions and recommendation
In the current study, the seroprevalence of the two common viral hepatitis infections was intermediate, and the rate of HBV and HCV particularly can be considered in the intermediate endemic category of the WHO classification scheme. Collaboration amongst various stakeholders is essential to reducing the prevalence of infections among pregnant women. It is recommended that pregnant women undergo regular screening, that women of childbearing age receive the Hepatitis B vaccine, and that health education programs raising awareness of HBV and HCV should take place. Furthermore, we recommend studies be conducted to compare the real infections of HBV and HCV by using more advanced laboratory techniques among pregnant women living in rural and urban areas of Ethiopia.
Data availability
The datasets generated and/or analysed during the current study are not publicly available due ongoing analyssi but are available from the corresponding author on reasonable request.
Abbreviations
- Anti-HCV:
-
Anti Hepatitis C virus Antibody
- ANC:
-
Antenatal Care
- HBsAg:
-
Hepatitis B surface antigen
- HBV:
-
Hepatitis B virus
- HCV:
-
Hepatitis C virus
- WHO:
-
World Health Organization
References
Averhoff FM, Glass N, Holtzman D. Global burden of hepatitis C: considerations for healthcare providers in the United States. Clin Infect Dis. 2012;55(suppl1):S10–5.
Organization WH. Global hepatitis report 2017: executive summary. World Health Organization; 2017. URLs: https://www.who.int/publications/i/item/9789241565455
Organization WH. Prevention and control of viral hepatitis infection: framework for global action. World Health Organization; 2012. URLs: https://www.who.int/publications/i/item/prevention-and-control-of-viral-hepatitis-infection-framework-for-global-action
Perz JF, Armstrong GL, Farrington LA, Hutin YJ, Bell BP. The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide. J Hepatol. 2006;45(4):529–38.
Blach S, Zeuzem S, Manns M, Altraif I, Duberg A-S, Muljono DH, et al. Global prevalence and genotype distribution of hepatitis C virus infection in 2015: a modelling study. Lancet Gastroenterol Hepatol. 2017;2(3):161–76.
World Health Organization. Progress report on HIV vhastiaftghss, 2016–2024. World Health Organization. 2024. URLs: https://www.who.int/news-room/fact-sheets
Organization WH. Combating hepatitis B and C to reach elimination by 2030: advocacy brief. World Health Organization; 2016. URLs: https://www.who.int/publications/i/item/combating-hepatitis-b-and-c-to-reach-elimination-by-2030
Schweitzer A, Horn J, Mikolajczyk RT, Krause G, Ott JJ. Estimations of worldwide prevalence of chronic hepatitis B virus infection: a systematic review of data published between 1965 and 2013. Lancet. 2015;386(10003):1546–55.
Organization WH. Global hepatitis report 2017: World Health Organization; 2017. URLs: https://www.who.int/publications/i/item/9789240027077
Organization WH. Global policy report on the prevention and control of viral hepatitis in WHO Member States. 2013. URLs: https://iris.who.int/bitstream/handle/10665/85397/9789241564632_eng.pdf
Coppola N, De Pascalis S, Onorato L, Calò F, Sagnelli C, Sagnelli E. Hepatitis B virus and hepatitis C virus infection in healthcare workers. World J Hepatol. 2016;8(5):273.
Hofstraat S, Falla A, Duffell E, Hahné S, Amato-Gauci A, Veldhuijzen I, et al. Current prevalence of chronic hepatitis B and C virus infection in the general population, blood donors and pregnant women in the EU/EEA: a systematic review. Epidemiol Infect. 2017;145(14):2873–85.
Geta M, Yizengaw E, Getaneh Z, Getahun T. Seroprevalence of hepatitis B virus infection among patients attending at Addis Alem Primary Hospital, Bahir Dar, Northwest Ethiopia. Int J Gen Med. 2021:405–11.
Elkhateeb R, Hassan K. Prevalence of hepatitis B and C in pregnant ladies and their neonates in Minia governorate. Int J Pregnancy Child Birth. 2018;4(1):71–83.
Giri S, Sahoo S, Angadi S, Afzalpurkar S, Sundaram S, Bhrugumalla S. Seroprevalence of hepatitis B virus among pregnant women in India: a systematic review and meta-analysis. J Clin Experimental Hepatol. 2022.
Fernandes CNS, Alves MM, Souza MLd, Machado GA, Couto G, Evangelista RA. Prevalence of hepatitis B and C seropositivity in pregnant women. Revista Da Escola De Enfermagem Da USP. 2014;48:89–96.
Dabsu R, Ejeta E. Seroepidemiology of hepatitis B and C virus infections among pregnant women attending antenatal clinic in selected health facilities in East Wollega Zone, West Oromia, Ethiopia. BioMed research international. 2018;2018.
Zhao X, Bai X, Xi Y. Intrauterine infection and mother-to-child transmission of hepatitis B virus: route and molecular mechanism. Infect Drug Resist. 2022:1743–51.
Umare A, Seyoum B, Gobena T, Haile Mariyam T. Hepatitis B virus infections and associated factors among pregnant women attending antenatal care clinic at Deder Hospital, Eastern Ethiopia. PLoS ONE. 2016;11(11):e0166936.
Bancha B, Kinfe AA, Chanko KP, Workie SB, Tadese T. Prevalence of hepatitis B viruses and associated factors among pregnant women attending antenatal clinics in public hospitals of Wolaita Zone, South Ethiopia. PLoS ONE. 2020;15(5):e0232653.
Atalay AA, Abebe RK, Dadhi AE, Bededa WK. Seroprevalence of hepatitis B virus among pregnant women attending antenatal care in Dilla University Referral Hospital Gedio Zone, Ethiopia; health facility based cross-sectional study. PLoS ONE. 2021;16(3):e0249216.
Tanga AT, Teshome MA, Hiko D, Fikru C, Jilo GK. Sero-prevalence of hepatitis B virus and associated factors among pregnant women in Gambella hospital, South Western Ethiopia: facility based cross-sectional study. BMC Infect Dis. 2019;19(1):1–7.
Bafa TA, Egata AD. Seroepidemiological patterns and predictors of hepatitis B, C and HIV viruses among pregnant women attending antenatal care clinic of Atat Hospital, Southern Ethiopia. SAGE Open Med. 2020;8:2050312119900870.
Dessalegn Mekonnen B. Prevalence of hepatitis C virus infection among pregnant women in Ethiopia: a systematic review and meta-analysis. Adv Prev Med. 2021;2021:1–8.
Molla S, Munshea A, Nibret E. Seroprevalence of hepatitis B surface antigen and anti HCV antibody and its associated risk factors among pregnant women attending maternity ward of Felege Hiwot Referral Hospital, northwest Ethiopia: a cross-sectional study. Virol J. 2015;12:1–9.
Dagnew M, Million Y, Gizachew M, Eshetie S, Yitayew G, Asrade L et al. Hepatitis B and C viruses’ infection and associated factors among pregnant women attending antenatal care in hospitals in the Amhara national regional state, Ethiopia. International journal of Microbiology. 2020;2020.
McMahon BJ, Alberts SR, Wainwright RB, Bulkow L, Lanier AP. Hepatitis B–related sequelae: prospective study in 1400 hepatitis B surface antigen–positive Alaska native carriers. Arch Intern Med. 1990;150(5):1051–4.
Reddick K, Jhaveri R, Gandhi M, James A, Swamy G. Pregnancy outcomes associated with viral hepatitis. J Viral Hepatitis. 2011;18(7):e394–8.
Safir A, Levy A, Sikuler E, Sheiner E. Maternal hepatitis B virus or hepatitis C virus carrier status as an independent risk factor for adverse perinatal outcome. Liver Int. 2010;30(5):765–70.
Gambarin-Gelwan M. Hepatitis B in pregnancy. Clin Liver Dis. 2007;11(4):945–63.
Zahran KM, Badary MS, Agban MN, Aziz NHA. Pattern of hepatitis virus infection among pregnant women and their newborns at the women’s health center of Assiut University, Upper Egypt. Int J Gynecol Obstet. 2010;111(2):171–4.
Shiferaw F, Letebo M, Bane A. Chronic viral hepatitis: policy, regulation, and strategies for its control and elimination in Ethiopia. BMC Public Health. 2016;16(1):1–13.
Belyhun Y, Maier M, Mulu A, Diro E, Liebert UG. Hepatitis viruses in Ethiopia: a systematic review and meta-analysis. BMC Infect Dis. 2016;16:1–14.
Bane A, Patil A, Khatib M. Healthcare cost and access to care for viral hepatitis in Ethiopia. Int J Innov Appl Stud. 2014;9(4):1718.
Nelson NP, Easterbrook PJ, McMahon BJ. Epidemiology of hepatitis B virus infection and impact of vaccination on disease. Clin Liver Dis. 2016;20(4):607–28.
Mamuye B, Gobena T, Oljira L. Hepatitis B virus infection and associated factors among pregnant women attending antenatal clinics in West Hararghe public hospitals, Oromia region, Ethiopia. Pan Afr Med J. 2020;35.
Kampe A, Kannaiyan Abbai M, Tilahun D, Daka D, Aliyo A, Dedecha W, et al. Seroprevalence of hepatitis B virus infection and associated factors among pregnant women attending antenatal care at public hospitals in Borena Zone, Southern Ethiopia. Health Serv Res Managerial Epidemiol. 2023;10:23333928231161946.
Eba S, Kejela G, Tamiru A. Seroprevalence of hepatitis B virus and associated factors among pregnant women attending antenatal care services at public health facilities in Nekemte Town. International Journal of Reproductive Medicine. 2021;2021.
Kenea D, Lemessa F. Assessment of hepatitis B virus infection and associated factors among pregnant women attending antenatal care service in Bishoftu Town, South East, Ethiopia. Am J Health Res. 2020;8(2):11–7.
Demeke G, Ayalneh GM, Shiferaw AA, Toru M, Dilnessa T. Sero-prevalence and associated factors of hepatitis B virus among pregnant women at north West Ethiopia: an institution-based cross-sectional study. Int J Gen Med. 2021:2799–805.
Gebretsadik D, Assefa M, Fenta GM, Daba C, Ali A, Tekele SG. High seroprevalence of hepatitis B and C virus infections among pregnant women attending antenatal clinic in Borumeda General Hospital, northeast Ethiopia. BioMed Research International. 2022;2022.
Kinfe H, Sendo EG, Gebremedhin KB. Prevalence of hepatitis B virus infection and factors associated with hepatitis B virus infection among pregnant women presented to antenatal care clinics at Adigrat General Hospital in Northern Ethiopia. Int J Women’s Health. 2021:119–27.
Roble AK, Roba KT, Mengistie B, Abdurke Kure M. Seroprevalence of hepatitis B virus and associated factors among pregnant women attending antenatal care in public health facilities in Jigjiga Town, Eastern Ethiopia. Int J Women’s Health. 2021:1299–310.
Geda YF, Desse H, Gesesse MM, Berhe TM. Hepatitis B surface antigen and associated factors among mothers who had antenatal care contact in Attat Hospital, southern Ethiopia. SAGE Open Med. 2021;9:20503121211024462.
Metaferia Y, Dessie W, Ali I, Amsalu A. Seroprevalence and associated risk factors of hepatitis B virus among pregnant women in southern Ethiopia: a hospital-based cross-sectional study. Epidemiol Health. 2016;38.
Tadiwos MB, Kanno GG, Areba AS, Kabthymer RH, Abate ZG, Aregu MB. Sero-prevalence of hepatitis B virus infection and associated factors among pregnant women attending antenatal care services in Gedeo Zone, Southern Ethiopia. J Prim Care Community Health. 2021;12:2150132721993628.
Kirbak ALS, Omolo J, Idris H, Usman A, Mbabazi WB. Sero-prevalence for hepatitis B virus among pregnant women attending antenatal clinic in Juba Teaching Hospital, Republic of South Sudan. Pan Afr Med J. 2017;26.
Mudardum AH, Mohammed AA. Prevalence and risk factors for hepatitis B infection among pregnant women attending antenatal clinic in UM dafog area, South Darfur state, Sudan. Sudan J Med Sci. 2019;14(3):116–25.
Manyahi J, Msigwa Y, Mhimbira F, Majigo M. High sero-prevalence of hepatitis B virus and human immunodeficiency virus infections among pregnant women attending antenatal clinic at Temeke municipal health facilities, Dar Es Salaam, Tanzania: a cross sectional study. BMC Pregnancy Childbirth. 2017;17:1–6.
Bittaye M, Idoko P, Ekele BA, Obed SA, Nyan O. Hepatitis B virus sero-prevalence amongst pregnant women in the Gambia. BMC Infect Dis. 2019;19(1):1–8.
Dortey BA, Anaba EA, Lassey A, Damale N, Maya ET. Seroprevalence of Hepatitis B virus infection and associated factors among pregnant women at Korle-Bu Teaching Hospital, Ghana. PLoS ONE. 2020;15(4):e0232208.
Taheu CN, Nguwoh PS, Demtaley A, Olinga PZ, Fokam J. Seroprevalence of hepatitis B virus using HBV-5 rapid panel test and associated factors amongst pregnant women attending antenatal care in Garoua, North-Cameroon. Eur J Med Health Sci. 2022;4(1):114–9.
Musa B, Bussell S, Borodo M, Samaila A, Femi O. Prevalence of hepatitis B virus infection in Nigeria, 2000–2013: a systematic review and meta-analysis. Niger J Clin Pract. 2015;18(2):163–72.
Dagnew M, Million Y, Gizachew M, Eshetie S, Yitayew G, Asrade L, et al. Hepatitis B and C viruses’ infection and associated factors among pregnant women attending antenatal care in hospitals in the Amhara National Regional State, Ethiopia. Int J Microbiol. 2020;2020:8848561.
Gedefaw G, Waltengus F, Akililu A, Gelaye K. Risk factors associated with hepatitis B virus infection among pregnant women attending antenatal clinic at Felegehiwot referral hospital, Northwest Ethiopia, 2018: an institution based cross sectional study. BMC Res Notes. 2019;12:1–7.
Zenebe Y, Mulu W, Yimer M, Abera B. Sero-prevalence and risk factors of hepatitis B virus and human immunodeficiency virus infection among pregnant women in Bahir Dar City, Northwest Ethiopia: a cross sectional study. BMC Infect Dis. 2014;14:1–7.
Zemenu AN, Daniel GD. Seroprevalence of hepatitis B surface antigen and associated risk factors among pregnant women in Gandhi Memorial Hospital, Addis Ababa. J Public Health Epidemiol. 2019;11(6):123–9.
Wabe Y, Reda D, Ali M. Seroprevalence of syphilis and HBV among pregnant women at Saint Paul s hospital millennium medical college, Addis Ababa Ethiopia. Prim Health Care: Open Access. 2021;11(7):1–6.
Firde M, Mosa H, Ahmed R, Demelash M, Endale F. Sero-prevalence and risk factors associated with hepatitis B virus infections among pregnant women at Shone Hospital, Southern Ethiopia: a cross-sectional study. J Midwifery Reproductive Health. 2022;10(1).
Agazhu HW, Robele MN, Ayele IT. Prevalence and associated factors of hepatitis B virus infection among pregnant women attending antenatal care at Agena health center, South Ethiopia, 2019: a cross sectional study. 2019.
Wakjira M, Darega J, Oljira H, Tura MR. Prevalence of hepatitis B virus and its associated factors among pregnant women attending antenatal care in Ambo town, Central Ethiopia: a cross-sectional study. Clin Epidemiol Global Health. 2022;15:101054.
Nyamusi MM, M’Imunya JM, Muvunyi CM, Habtu M. Factors associated with Hepatitis B surface antigen seropositivity among pregnant women in Kigali, Rwanda: a cross sectional study. 2017.
Mugabiirwe N, Kalyetsi R, Ayella R, Obote J, Ssedyabane F. Hepatitis B virus infection and HBeAg positivity among pregnant women in South West Uganda. Afr J Lab Med. 2022;11(1):1–6.
Israr M, Ali F, Nawaz A, Idrees M, Khattak A, Ur Rehman S, et al. Seroepidemiology and associated risk factors of hepatitis B and C virus infections among pregnant women attending maternity wards at two hospitals in Swabi, Khyber Pakhtunkhwa, Pakistan. PLoS ONE. 2021;16(8):e0255189.
Jamil A, Hamid S, Aziz Q. Prevalence of hepatitis B among pregnant women. J Islamabad Med Dent Coll. 2018;7(2):128–31.
Cetin S, Cetin M, Turhan E, Dolapcioglu K. Seroprevalence of hepatitis B surface antigen and associated risk factors among pregnant women. J Infect Developing Ctries. 2018;12(10):904–9.
Mudji J, Madinga B, Horsmans Y. Seroprevalence of viral hepatitis B and C and knowledge of the hepatitis B virus among pregnant women attending prenatal care in the Democratic Republic of Congo. Am J Trop Med Hyg. 2021;104(3):1096.
Makvandi M. Update on occult hepatitis B virus infection. World J Gastroenterol. 2016;22(39):8720.
Taye BD, Kassa T, Teshager L, Kedir R, Yeshanew AG, Aseffa A et al. Prevalence and associated risk factors of hepatitis B and C virus infections among mothers in Jimma, South West Ethiopia: a community-based study. Ethiop Med J. 2019;57.
Ephraim R, Donko I, Sakyi SA, Ampong J, Agbodjakey H. Seroprevalence and risk factors of hepatitis B and hepatitis C infections among pregnant women in the Asante Akim North Municipality of the Ashanti region, Ghana; a cross sectional study. Afr Health Sci. 2015;15(3):709–13.
Zenebe Y, Mulu W, Yimer M, Abera B. Sero-prevalence and risk factors of hepatitis C virus infection among pregnant women in Bahir Dar City, Northwest Ethiopia: cross sectional study. Pan Afr Med J. 2015;21(1).
Seid M. Sero-prevalence of HBV and HCV infections among pregnant women attending antenatal care clinic at Dessie Referral Hospital, Ethiopia. Adv Life Sci Health. 2014;1(2):109.
Oti V, Mohammed I, Ibrahim Y, Ibrahim C, Orok I. Epidemiologic survey of HBV, HCV and HIV infections in a pregnant women population in Central Nigeria: a cross-sectional study. J Infect Dis Epidemiol. 2021;7:194.
Oladeinde BH, Omoregie R, Olley M, Anunibe JA, Oladeinde OB. Hepatitis B and C viral infections among pregnant women in a rural community of Nigeria. Int J Basic Appl Virol. 2012;1(1):01–5.
Omatola CA, Lawal C, Omosayin DO, Okolo M-lO, Adaji DM, Mofolorunsho CK, et al. Seroprevalence of HBV, HCV, and HIV and associated risk factors among apparently healthy pregnant women in Anyigba, Nigeria. Viral Immunol. 2019;32(4):186–91.
Xiong H, Rong X, Wang M, Xu R, Huang K, Liao Q, et al. HBV/HCV co-infection is associated with a high level of HCV spontaneous clearance among drug users and blood donors in China. J Viral Hepatitis. 2017;24(4):312–9.
Acknowledgements
We heartfelt thank the Guhala primary hospital Laboratory staff for their encouragement and technical support. We also very grateful and would like to extend our heartfelt thanks and appreciation to the study participants.
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DB, GA, WA, and AA play a great role in the conception, study design, execution, acquisition of data, analysis and interpretation, EF, MK, MF, AJ, and TM took part in data curation, DB, and AA took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work. All authors reviewed the manuscript.
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Ethical approval was obtained ethical clearance from the University of Gondar; School of Biomedical and Laboratory Sciences ethical review committee with reference number SBMLS/2934/2022. We conducted the study by following the Declaration of Helsinki. Before data collection, we explained the study objectives to the heads of the hospital director and the serology laboratory personnel. Due to the nature of this retrospective study and the preserved anonymity of patients, a waiver of informed consent was obtained. The waivers of informed consent were the University of Gondar; School of Biomedical and Laboratory Sciences and Guhala primary hospital. To ensure the confidentiality of information from the participant’s records, we didn’t record any personal identifiers on the data collection sheet, and secured data from participant records were not available to anyone except for the investigators.
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Belete, D., Fekadie, E., Kassaw, M. et al. Seroprevalence of hepatitis B virus and hepatitis C virus infection among pregnant women attending antenatal care at Guhala Primary Hospital, Northwestern Ethiopia. BMC Pregnancy Childbirth 24, 512 (2024). https://doi.org/10.1186/s12884-024-06714-6
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DOI: https://doi.org/10.1186/s12884-024-06714-6