Correction to: BMC Genomics 22, 809 (2021)
https://doi.org/10.1186/s12864-021-08132-w
Following publication of the original article [1], it was reported that there were errors in the x- and y-axes of Figs. 4, 6 and 7.
Sensitivity of different terminal modification TSO library construction methods. A The number of genes detected at 5 pg and 0.5 pg RNA inputs in different terminal modification TSO. B The ratio of the detected genes in the cell marker gene database of mice at 5 pg and 0.5 pg RNA inputs in different terminal modification TSO. C Precision for detecting genes in different terminal modification TSO. D Sensitivity for detecting genes in different terminal modification TSO. E, F The median number of genes detected per sample when downsampling total read counts to the indicated depths at 5 pg and 0.5 pg RNA inputs
The effect of RNA with different template structures on gene detection. A The number of genes detected in the different structure of mRNA templates. B The ratio of the detected genes in the cell marker gene database of mice at the different structures of mRNA templates. C, D Number of genes detected in different expression levels binned by standardized expression FPKM at the different structures of mRNA templates. E, F Scatter plots show the correlation between different replicates and mRNA structure for 5 pg RNA inputs. G, H Scatter plots show the correlation between different replicates and mRNA structure for 0.5 pg RNA inputs
Effect of RNA with different template structures on the accuracy and sensitivity of sequencing. A Precision for detecting genes at the different structures of mRNA templates. B Sensitivity for detecting genes at the different structures of mRNA templates. C, D The median number of genes detected per sample when downsampling total read counts to the indicated depths at 5 pg and 0.5 pg RNA inputs
In Fig. 4 the x axes of panels E and F were missing ‘2’ and the y axes contained a ‘$’ and “#” symbol after the values respectively.
In Fig. 6, in panel H the y axis contained a ‘$’ symbol after the reported values.
In Fig. 7, the x and y axis of panel C erroneously contained the ‘$’ symbol and in panel D both axes contained the “#” symbol.
The correct figures are presented in this Correction and the original article [1] has been corrected.
Reference
Jia E, Shi H, Wang Y, et al. Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues. BMC Genomics. 2021;22:809. https://doi.org/10.1186/s12864-021-08132-w.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Jia, E., Shi, H., Wang, Y. et al. Correction to: Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues. BMC Genomics 23, 108 (2022). https://doi.org/10.1186/s12864-022-08322-0
Published:
DOI: https://doi.org/10.1186/s12864-022-08322-0