Imaging of Biological Cells Using Luminescent Silver Nanoparticles
- 3.1k Downloads
The application of luminescent silver nanoparticles as imaging agents for neural stem and rat basophilic leukemia cells was demonstrated. The experimental size dependence of the extinction and emission spectra for silver nanoparticles were also studied. The nanoparticles were functionalized with fluorescent glycine dimers. Spectral position of the resonance extinction and photoluminescence emission for particles with average diameters ranging from 9 to 32 nm were examined. As the particle size increased, the spectral peaks for both extinction and the intrinsic emission of silver nanoparticles shifted to the red end of the spectrum. The intrinsic photoluminescence of the particles was orders of magnitude weaker and was spectrally separated from the photoluminescence of the glycine dimer ligands. The spectral position of the ligand emission was independent of the particle size; however, the quantum yield of the nanoparticle-ligand system was size-dependent. This was attributed to the enhancement of the ligand’s emission caused by the local electric field strength’s dependence on the particle size. The maximum quantum yield determined for the nanoparticle-ligand complex was (5.2 ± 0.1) %. The nanoparticles were able to penetrate cell membranes of rat basophilic leukemia and neural stem cells fixed with paraformaldehyde. Additionally, toxicity studies were performed. It was found that towards rat basophilic leukemia cells, luminescent silver nanoparticles had a toxic effect in the silver atom concentration range of 10–100 μM.
KeywordsPhotoluminescence Surface plasmon resonance Plasmon radiative decay Surface-enhanced photoluminescence Nanoparticles Bio-imaging Neural stem cells Rat basophilic leukemia cells
Noble metal nanoparticles (NPs) exhibit unique electronic and optical properties when excited by external light because of the collective and coherent excitations of their conduction electrons known as surface plasmon resonance (SPR) [1, 2]. SPR excitations in metal NPs can find applications in biosensing, [3, 4] bio-imaging,  photothermal therapy, , and in plasmonic rulers, [7, 8] to name just a few. Silver NPs exhibit the strongest SPR response when compared to other noble metals, such as gold or copper. The size of the metal NPs plays a crucial role in their photoluminescence emission due to the strong size dependence of the local field enhancement factor [9, 10, 11, 12]. The size dependence of SPR in noble metal NPs is a well-known phenomenon;  however, the size-dependent photoluminescence (PL) has been studied much less, despite its potential applications in solar cells,  SERS, , and bio-imaging [5, 14].
In the work presented here, the SPR in silver NPs was exploited to control their intrinsic PL emission, as well as to enhance the PL of glycine ligands. In previous studies, the intrinsic PL of NPs has been rationalized as SPR-enhanced interband electronic transitions,  or alternatively direct plasmon emission due to the radiative damping of the SPR . Previous reports have indicated a quantum yield (QY) for the intrinsic PL of silver NPs as high as 1.2 × 10−2 for 8 nm NPs, and 4.6 × 10−3 for 11 nm NPs,  a somewhat lower quantum yield 10−4–10−5 for 5 nm gold NPs, , and even lower 10−5 for 17 nm copper NPs . All of the mentioned QY values are orders of magnitude larger than that of 10−10 for bulk metals .
Another SPR-related effect, the enhancement of a ligand’s PL from a nanoparticle’s surface is caused by the strong local field at the NPs surface, whereby the particle enhances the incoming and outgoing photons. The surface enhancement of Raman scattering for Rhodamine 6G was reported to be most efficient for 50-nm-diameter NP . In the experiments presented here, evidence of the connection between SPR excitations in silver NPs and their intrinsic PL signal was found. This connection was then compared to the surface-enhanced PL of the glycine dimer ligands, with the goal of developing robust, non-toxic, stable, and bright fluorescent biomarkers.
All experiments were performed with chemicals and solvents of reagent grade and were used without further modification or purification. For synthetic procedures, glycine (CAS: 56-40-6, Reagent Plus 99 %) and silver nitrate (CAS: 7761-88-8, ACS Reagent 99+ %) were purchased from Sigma-Aldrich. The quantum yield standard, quinine sulfate (CAS: 207671-44-1, meets USP testing specifications), was purchased from Sigma-Aldrich. Aqueous quinine standards were prepared in 0.05 M sulfuric acid (H2SO4(aq), CAS: 7664-93-9, Alfa Aesar). Distilled deionized water (pH 6.3) was used for all experiments and reagent dilutions.
The nanoparticles used for this study were synthesized based on a procedure previously described in the literature . Silver nitrate and glycine (1-to-10 mass ratio) were dissolved in distilled deionized water in order to form an intimate mixture. The water was then evaporated under low pressure to form a solid crystalline reaction mixture. After solvent evaporation, a solid phase thermal reduction was performed on the crystalline mixture at 445 K for 5 min. During this procedure, glycine-dimer-coated silver nanoparticles were formed via a reduction-oxidation reaction, while the solid glycine matrix controlled the size of these particles. The resulting product was dissolved in water and sonicated for 24 h to break apart any aggregates. The absorption and emission spectra, obtained every hour during the sonication procedure, showed no evidence of additional product formation (such as nanoclusters). The product was then filtered through a 2-μm-core-diameter filter and subjected to a centrifugation procedure that separated the insoluble aggregates and free glycine molecules from the glycine-dimer-coated nanoparticles.
The C17.2 cell line adopted from the cerebellum of newborn mice was supplied by Dr. Evan Y. Snyder from Harvard Medical School, Boston, MA. C17.2 neural stem cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) and supplemented with HEPES, penicillin streptomycin (Pen-Strep), l-glutamine, 10 % fetal bovine serum (FBS), and 5 % normal horse serum.
Rat basophilic leukemia (RBL) cells (RBL-2H3; ATCC number CRL-2256) were maintained in minimal essential medium (MEM) supplemented with 10 % FBS, 1 % Pen-Strep, and 1 % l-glutamine (L-glut).
TEM Size and Shape Characterization
Samples containing the desired NPs of different sizes were characterized by a JEOL JEM-100CX II transmission electron microscope. Images of selected samples and their size distributions are shown in Fig. 1. As was expected, the largest RCF applied resulted in the smallest NPs to be found in the supernatant (Fig. 1a), and the lowest RCF resulted in the largest NPs to be dragged into pellet (Fig. 1d).
Relative QY was determined by comparing the photoluminescence from glycine-dimer-coated silver NPs to the well-characterized QY standard, quinine (in 0.05 M H2SO4(aq)). Each sample of NPs was matched in its optical density to that of the standard at the excitation wavelength of 366 nm (the QY of the quinine is known to be 0.54 ± 0.003 when excited at 366 nm) . This condition guarantees that the NPs in the sample and the QY standard absorb the same amount of light upon excitation. Once the optical density of the standard and NP samples was matched, the PL spectra were recorded and the relative quantum yields of each sample were calculated using the ratio of the areas under their PL spectra.
A Leica TCS SP5 confocal microscope was used to obtain fluorescence and transmission images of neural and RBL cells incubated with NPs. The C17.2 cells were fixed using 1 % and RBL using 4 % paraformaldehyde for 20 min. The paraformaldehyde was then removed and cells were washed with PBS, covered with the media, and imaged with a ×40 oil objective. The excitation laser wavelength was 405 nm, and the emission channel was set to 420–535 nm. A 0.2-mL aqueous solution of fluorescent glycine-dimer-coated NPs with average diameter of 9 ± 4 nm was added to the fixed cells, which were contained in 2 mL of media. Imaging was performed every 15 min, starting immediately after addition of NPs. Every set of images included a z-stack of 100 focal planes. Images obtained of cells taken during NP incubation are shown on Figs. 6 and 7.
Results and Discussion
Intrinsic Silver NP Photoluminescence
After size separation, the samples contained NPs of mean silver core diameters 9 ± 4, 14 ± 5, 23 ± 4, and 32 ± 8 nm (Fig. 1). The smaller particles were near-spherical, while the larger particles were more irregular (Fig. 1a–d).
Optical properties of the particles were then studied to determine their size dependence. The intrinsic silver NP’s PL spectra were obtained with the excitation wavelength of 250 nm. This excitation wavelength is within the spectral region of the interband absorption for silver (the edge of the interband absorption is at 388 nm) . Under a 250 nm excitation, the glycine dimer’s PL was not excited, and only the size-dependent intrinsic PL of the NPs was observed. It was found that as the NP’s diameter increased, the PL peak (Fig. 2c) was spectrally shifted towards the red end of the spectrum, similar to that of the SPR-related peak in absorbance, shown in Fig. 2b. This shift in absorbance is well-known and has been rationalized by the so-called polaritonic effect caused by a phase retardation of the electromagnetic waves (also known as extrinsic or electrodynamic effect) . Multiple experimental studies confirm this effect and have shown a red shift of the SPR peak in the absorbance spectrum as the diameter of the NP increases (for diameters >10 nm) ; however, the same shift in the intrinsic PL has been previously reported in very few studies .
With the observed the shared trend in size dependence and spectral shifts of both the PL and absorbance (also called extinction or optical density (OD)) spectra (Fig. 2a) indicate that the intrinsic PL of the NPs is due a radiative decay of the SPR. The radiative decay of the SPR in noble metal NPs is also known as plasmon emission, or plasmon scattering, because of its very fast (a few femtoseconds) decay . It has been stated that the red shift of both OD and PL spectra is an indicator of the plasmon emission, as opposed to the interband emission mechanism . It is worth noting that some previous studies have observed PL due to both, the interband transitions and plasmon emission in noble metal NPs [11, 15, 24].
Comparison of the PL intensity from NPs of different sizes was performed for samples matched in OD at 250 nm. This guaranteed that the same number of photons was absorbed by all samples. The intensity of intrinsic PL from the silver NPs increased as their size increased. This is further evidence towards the rationalization of radiation damping as the source of PL in the metal NPs.
Briefly, the mechanism of PL due to a radiative decay is as follows: First, the interband electronic transitions are excited by the incident light. This excitation relaxes into the SPR modes, and the SPR then radiates or emits this energy as photons (radiatively decays) . Seen as photoluminescence from the NPs, the energy radiated during this decay process is size-dependent because the SPR is also size-dependent. Evidence for such a PL mechanism in noble metal NPs has been shown in previous studies [24, 25, 26].
Despite the low QY observed from the intrinsic PL of metal NPs, much of the recent literature suggest its potential in bio-imaging and other applications . An alternative use of SPR excitations in silver NPs can be found in the enhancement of PL from fluorescent ligands on the surface of a NP. In the next section, the size-dependent surface-enhanced PL of glycine dimer ligands is reported. Further discussion on how radiative decay affects the surface enhancement of the ligand PL is also provided.
Surface-Enhanced Photoluminescence of Glycine Dimers
Glycine was initially chosen as a NP-ligand because of the bio-compatibility of this amino acid. The bio-compatibility of the cyclic glycine dimers is yet to be studied.
It was found that the quantum yield for the glycine-dimer-NPs was non-monotonically dependent on the NP’s diameter, as it reached a maximum for the 23-nm-core-diameter NPs. Mainly, for the NP-ligand system, with a mean core diameter of 9 nm, the QY was 2.7 %; for 23-nm core, it was 5.2 %; and for 32 nm, it was 1.5 %. The dimer-NP emission spectral position, however, did not show any noticeable size dependence (Fig.5), which supports the hypothesis that the origin of this PL is glycine dimer’s emission.
In order to evaluate the possible use of glycine-dimer-NPs as biomarkers, fixed neural stem and RBL cells were incubated with NPs. Fluorescence confocal microscopy was then used to study the particles’ penetration into the cells.
In living cells, the membrane selectively controls transport of ions, large bio-molecules, and NPs into and out of the cell. The mechanisms of this transport depend on the size of the object being transported and include active endocytosis/exocytosis and passive transport (this mechanism is predominant in red blood cells). Internalization of a NP not only depends on their size, shape, surface charge, and surface chemistry  but also depends on the cell-specific parameters, such as cell type or cell cycle phase .
In this study, cells were fixed, then stained with NPs (as often performed in immunoassays), and hence only passive transport could take place. According to Van Lehn et al., pore formation in the membrane can be avoided if the ligand layer on the NP is able to adjust to the membrane, allowing surface charges to rearrange, so that the NP appears hydrophobic . As the ligand layer around smaller particles contains a large amount of free volume because of the high curvature, ligand fluctuations are maximized, allowing small NPs to more easily penetrate a membrane . We note that another possibility of NP internalization in this study is the partial permeabilization of a cell membrane by paraformaldehyde. Further study of the size-dependent NP internalization by live cells would be of great interest.
Toxicity for the RBL Cells
Silver NPs are known to have antibacterial properties . They are toxic towards bacterial cells but non-toxic towards mammalian cells up to relatively high concentrations . This property of NPs is becoming more of interest, as antibiotic resistance in bacteria has become a major clinical and public health problem . The main mechanism of NP toxicity is inhibition of the cell proliferation due to NP decomposition onto Ag+ ions. This decomposition is a result of dissolution of NPs in the liquid medium and digestion of NPs upon intercellular uptake. Ag+ ions can bind to thiol-containing molecules within the cell . It has been shown that it leads to cell membrane detachment from the cell wall, cell wall damage, cell shrinkage and dehydration, and stress response via accumulation of DNA in the center of the cell . In Escherichia coli bacteria, silver NPs cause cell proliferation inhibition . Minimal inhibition concentration of Ag NPs has been determined to be 3.3 nM, which is relatively low compared to the Ag NPs concentration in our study (compare to 50 nM, for which the toxic effect was observed). Furthermore, other studies have observed that the toxicity of silver NPs versus Ag+ ions on mammalian cells showed significant toxic effects starting from 200 nM NP concentration , which suggests that our NPs are more toxic. Literature also suggests that other effects on the rat basophilic leukemia cells, such as degranulation, may occur . We would like to add, that according to the experimental evidence presented in this work the origin of the photoluminescence of silver nanoclusters reported in previous publication  must be glycine dimers, and not the intrinsic emission of the silver clusters, as we suggested before.
In conclusion, a correlation between the SPR-related peaks in the extinction and intrinsic emission spectra of silver nanoparticles of different sizes has been experimentally demonstrated. It was found that for NPs with an average diameter in the range of 9–32 nm, an increase in particle size leads to a red shift of the peak in both extinction and intrinsic photoluminescence spectra. These observations expand previous studies and prove the role of the SPR in the PL of noble metal NPs. The intrinsic particle PL emission was rationalized via radiative damping of the surface plasmon. In addition, the size-dependent quantum yield of the PL for the glycine dimer-coated NPs was studied. A quantum yield of the NP-glycine-dimer complex was shown to depend on silver particle's core size (maximum QY of (5.1 ± 0.1) % was estimated for averge NP's diameter 23+/-4 nm). Additionally, it was shown that NPs with average diameters of 9 ± 4 nm can be used for bio-imaging of fixed C17.2 neural stem cells as well as RBL cells. For RBL cells, nanoparticles were non-toxic up to a 10-μM silver concentration and inhibition of cell proliferation occurred at a concentration of 100 μM.
The authors thank Dr. Eugenia Olesnicky Killian, Dr. Kevin Tvrdy, and Sara Goldman for their help with the image processing and interpretation, rationalization of the results of the synthetic procedure, and critical reading of the manuscript, correspondingly. We acknowledge partial support from the UCCS Center for Biofrontiers Institute and the Butcher Foundation seed grant.
- 2.Brongersma ML (2008) Recent progress in plasmonics, Conference on Quantum Electronics and Laser Science (QELS) - Technical Digest SeriesGoogle Scholar
- 5.Braun GB, Friman T, Pang HB, Pallaoro A, De Mendoza TH, Willmore AMA, et al (2014) Etchable plasmonic nanoparticle probes to image and quantify cellular internalization. Nat Mater 13:904–911Google Scholar
- 8.Kravets VV, Ocola LE, Khalavka Y, Pinchuk AO. Polarization and distance dependent coupling in linear chains of gold nanoparticles. Appl Phys Lett 2015;106:053104.Google Scholar
- 11.Dulkeith E, Niedereichholz T, Klar TA, Feldmann J, Von Plessen G, Gittins DI, et al (2004) Plasmon emission in photoexcited gold nanoparticles. Phys Rev B: Condens Matter Mater Phys 70:205424Google Scholar
- 12.Dmitruk I, Blonskiy I, Pavlov I, Yeshchenko O, Alexeenko A, Dmytruk A, et al (2009) Surface plasmon as a probe of local field enhancement. Plasmonics 4:115–119Google Scholar
- 15.Yeshchenko OA, Dmitruk IM, Alexeenko AA, Losytskyy MY, Kotko AV, Pinchuk AO. Size-dependent surface-plasmon-enhanced photoluminescence from silver nanoparticles embedded in silica. Phys Rev B: Condens Matter Mater Phys 2009;79:235438.Google Scholar
- 17.Yeshchenko OA, Bondarchuk IS, Losytskyy MY. Surface plasmon enhanced photoluminescence from copper nanoparticles: influence of temperature. J Appl Phys 2014;116:054309-054309-8Google Scholar
- 25.Gaiduk A, Yorulmaz M, Orrit M (2011) Correlated absorption and photoluminescence of single gold nanoparticles. Chem Phys Chem 12:1536–1541Google Scholar
- 26.Zhang T, Lu G, Shen H, Shi K, Jiang Y, Xu D, et al. Photoluminescence of a single complex plasmonic nanoparticle. Scientific Reports. 2014;4:3867.Google Scholar
- 30.Shang L. Engineered nanoparticles interacting with cells: size matters in Nienhaus K (ed), BioMed Central, 2011.Google Scholar
- 32.Mahmoudi M, Saeedi-Eslami SN, Shokrgozar MA, Azadmanesh K, Hassanlou M, Kalhor HR, et al (2012) Cell “vision”: complementary factor of protein corona in nanotoxicology. Nanoscale 4:5461–5468Google Scholar
- 33.Verma A, Uzun O, Hu Y, Han HS, Watson N, Chen S, et al (2008) Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. Nat Mater 7:588–595Google Scholar
- 34.Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, et al (2007) Antimicrobial effects of silver nanoparticles. Nanomed: Nanotechnol Biol Med 3:95–101Google Scholar
- 35.Manshian BB, Pfeiffer C, Pelaz B, Heimerl T, Gallego M, Möller M, et al (2015) High-content imaging and gene expression approaches to unravel the effect of surface functionality on cellular interactions of silver nanoparticles. ACS Nano 9:10431–10444Google Scholar
- 40.Kravets V, Culhane K, Dmitruk I, Pinchuk A. Glycine-coated photoluminescent silver nanoclusters, Proceedings of SPIE, Vol. 8232, 82321AGoogle Scholar
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.