Abstract
Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.
Sammendrag
Ved at lade trypsin og diethylaminoethyl-dextran indgå i det faste substrat var det muligt at påvise bovin rotavirus (UK stammen) gennem plaque teknik. I kulturer af abeceller (BGM celler) blev der op-nået 1 mm store plaques, mens der i sekundaere kulturer af kalvenyre-celler regelmaessigt sås plaque af 3—4 mm st0rrelse.
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Butchaiah, G., Lund, E. Plaque Assay of Bovine Rotavirus. Acta Vet Scand 24, 362–373 (1983). https://doi.org/10.1186/BF03546710
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DOI: https://doi.org/10.1186/BF03546710