Background
Environmental factors play a major role in the rising prevalence of type 1 and type 2 diabetes mellitus. Cereulide is a lipophilic peptide that is often found at low concentrations in starchy food. It is a culprit to consider in this era of prepackaged meals.
Materials and methods
Mouse and rat insulin producing beta cell lines, MIN6 and INS-1E respectively, as well as whole mouse islets, isolated from 2 week old C57Bl/6J mice, were exposed to cereulide concentrations ranging from 0.05ng/ml to 5ng/ml for 24 and 72h. Cell death was evaluated by a Hoechst/Propidium Iodide assay, and compared to cell death in human hepatocellular HepG2 and monkey fibroblast-like COS-1 cells. Subsequently, MIN6 cells were exposed to low concentrations of cereulide (0.15 - 0.5 ng/ml) for 24h and glucose-stimulated insulin secretion was evaluated as well as mechanisms of toxicity by mRNA profiling, electron microscopy and caspase activation and cytochrome c release assay.
Results
Cereulide exposure caused cell death in MIN6, INS-1E and pancreatic islets, but not in HepG2 or COS-1E cells (Table 1). Caspase 3/7 activation confirmed the apoptotic cell death process. Glucose-stimulated insulin secretion decreased from 10.48 ± 3.33 fold to 2.01 ± 0.51 (P < 0.05) in MIN6 cells after 24h exposure with 0.25 ng/ml cereulide. Exposure to 0.25ng/ml cereulide induced markers of mitochondrial stress, including PUMA (p53 upregulated modulator of apoptosis; 271 ± 77 % of control; P < 0.05) but also markers of ER stress, such as CHOP (CCAAT/-enhancer-binding protein homologous protein; 641 ± 190 % of control; P < 0.01). EM revealed swelling and loss of mitochondria, and cytoplasmic cytochrome c release confirmed mitochondrial cell death signalling (360 ± 83 % of control after exposure to 0.5 ng/ml for 24h (P < 0.05).
Conclusion
Cereulide, a toxin frequently found in prepackaged or prepared starchy meals, increases levels of mitochondrial and ER stress markers in beta cells of rats and mice, even at low doses. In a dose dependent way, it also leads to impaired beta cell function and apoptosis. Cereulide might thus be involved in the current diabetes.
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Vangoitsenhoven, R., Rondas, D., Crèvecoeur, I. et al. Foodborne cereulide causes beta cell dysfunction and apoptosis. Arch Public Health 72 (Suppl 1), O8 (2014). https://doi.org/10.1186/2049-3258-72-S1-O8
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DOI: https://doi.org/10.1186/2049-3258-72-S1-O8