Abstract
Background
The vestibular system provides the primary input of our sense of balance and spatial orientation. Dysfunction of the vestibular system can severely affect a person's quality of life. Therefore, understanding the molecular basis of vestibular neuron survival, maintenance, and innervation of the target sensory epithelia is fundamental.
Results
Here we report that a point mutation at the phospholipase Cγ (PLCγ) docking site in the mouse neurotrophin tyrosine kinase receptor TrkB (Ntrk2) specifically impairs fiber guidance inside the vestibular sensory epithelia, but has limited effects on the survival of vestibular sensory neurons and growth of afferent processes toward the sensory epithelia. We also show that expression of the TRPC3 cation calcium channel, whose activity is known to be required for nerve-growth cone guidance induced by brain-derived neurotrophic factor (BDNF), is altered in these animals. In addition, we find that absence of the PLCγ mediated TrkB signalling interferes with the transformation of bouton type afferent terminals of vestibular dendrites into calyces (the largest synaptic contact of dendrites known in the mammalian nervous system) on type I vestibular hair cells; the latter are normally distributed in these mutants as revealed by an unaltered expression pattern of the potassium channel KCNQ4 in these cells.
Conclusions
These results demonstrate a crucial involvement of the TrkB/PLCγ-mediated intracellular signalling in structural aspects of sensory neuron plasticity.
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Background
The vestibular system consists of 5 end organs, which include 3 semicircular canals and their associated cristae involved in angular acceleration, and two otolith organs (the utricle and the saccule) involved in linear acceleration, including responses to gravity. Each of these end organs contains a sensory epithelium, which in mammals consists of two types of hair cells, namely type I and type II (50% each) that have distinct distribution patterns, polarity and physiology [1]. Bdnf is expressed in both types of differentiated hair cells in each of the sensory organs during embryonic and neonatal inner ear development [2].
Vestibular sensory neurons reside in the vestibular ganglia and are bipolar, sending a peripheral process (afferent) to the hair cells in their respective sensory epithelia, and a central process to the vestibular nuclei of the medulla [3]. Two different types of afferent fibers innervate the hair cells. Thick fibers innervate type I hair cells and form large calyceal endings, whereas thin fibers innervate type II hair cells and exclusively form bouton terminals. It has been clearly shown by studies of genetic mutations in mice that in the inner ear (which comprises the vestibular and cochlear systems) vestibular sensory neuron survival and innervations mainly depend on the neurotrophin receptor tyrosine kinase TrkB and its ligand BDNF [4].
BDNF/TrkB activates three main intracellular signalling cascades. The Ras/mitogen activated protein kinase (MAPK) pathway and the phosphoinositide 3-kinase (PI3K) pathway are activated primarily through SHC/FRS-2 binding to phosphorylated tyrosine (Y)515, whereas phosphorylated Y816 activates a calcium/calmodulin-dependent protein kinase II (CaMKII) pathway through PLCγ. We have previously described the creation of highly defined mouse models that carry tyrosine to phenylalanine (F) point mutations at either Y515 (TrkBSHC mice) or Y816 (TrkBPLC mice). Using these genetic models, we have been able to show that in the central nervous system the PLCγ docking site is necessary for TrkB-mediated hippocampal synaptic plasticity [5]. Moreover, a TrkB Y816F but not Y515F point mutation impairs both the acquisition of an associative learning task and long-term potentiation (LTP) between hippocampal neurons in vivo [6]. Recently, we have also shown that the Y816F point mutation in TrkB impairs acquisition of fear learning, amygdalar synaptic plasticity, and CaMKII signalling at synapses. In contrast, a Y515F point mutation affects consolidation but not acquisition of fear learning to tone, and also alters AKT activation [7]. In the peripheral nervous system, we have previously reported that a point mutation in the SHC binding site of TrkB only mildly affects vestibular sensory neuron survival, indicating that TrkB receptors promote long-term survival of sensory neurons mainly in a SHC site-independent manner [8]. In contrast, fiber growth was reduced, and target innervation by these sensory neurons was ultimately lost in TrkBSHC mice [9]. These results suggested that TrkB receptor signals that maintain target innervation require the SHC site. Here we have asked whether the PLCγ docking site of TrkB would be responsible for the fiber guidance role of TrkB reported in the inner ear [10], possibly through activation of TRPC channels as recently demonstrated in vitro [11, 12]. We find that the major function of the PLCγ site downstream of TrkB is fiber guidance, but surprisingly only inside the vestibular sensory epithelia, with limited effects on the survival of vestibular sensory neurons and growth of afferent processes toward the sensory epithelia. We also demonstrate that double point mutations in both the SHC and the PLCγ docking sites (referred to as TrkBD mice) [13] results in a loss of vestibular neurons that is essentially indistinguishable from that of Trkb null mice (Trkb-/- ), suggesting that the survival function of BDNF/TrkB is mediated predominantly through the cooperative action of these two docking sites, whereas the PLCγ site-activated signalling pathway(s) of TrkB is essential for the proper intraepithelial navigation of fibers. In addition, we show that the transformation of afferent dendritic boutons into calyces requires BDNF/TrkB signalling via the PLCγ docking site, revealing the requirement of this site for structural plasticity of sensory neurons.
Results
Vestibular neurons survive in the absence of a functional TrkB PLCγ site, but not in absence of both the PLCγ and the SHC sites
Previously we have shown that a point mutation at the SHC site of TrkB only partially affects the survival of inner ear vestibular neurons (25% loss) compared to control mice at postnatal day 7 (P7) [8]. Neurons remaining at P7 survived into adulthood, indicating that activation of pathways independent of the SHC site support the survival of vestibular neurons given that in the Trkb-/- mutants the majority of these neurons are already lost at birth [8, 9]. Vestibular ganglia of TrkbPLC/PLC mutants, which are homozygous for the Y816F point mutation, and control mice (TrkbWT/WT , TrkbPLC/+ ) were analysed to determine if the PLCγ site of TrkB supports vestibular neuron survival. Neuronal counts revealed a comparable number of sensory neurons in the vestibular ganglion of TrkbPLC/PLC mutants at P7 compared to the control genotypes (TrkbWT/WT , and TrkbPLC/+ ) (Fig. 1A). We then determined whether the two tyrosines comprising the SHC and the PLCγ docking sites together mediate the functions of Trkb in the vestibular ganglion. Homozygous double point mutant mice (TrkbD/D )[13] showed a loss of vestibular neurons equivalent to that of Trkb-/- mice (Fig. 1A-D) [14], and had a complete lack of afferent innervations to the posterior canal cristae with only an occasional fiber projecting to the anterior and horizontal cristae (Fig. 2A). The innervations to the utricle and saccule were reduced and closely resembled those of Bdnf-/- mice [15] and of Trkb-/- mice [16] previously reported. Similar effects were also obtained for the efferent innervations to the ear (Fig. 2B,C).
Previously, it was shown that deletion of Trkb or Bdnf reduces radial fiber density in the apex of the cochlea [16], a region that normally expresses high levels of BDNF during development [17]. Mice carrying a point mutation at the PLCγ site of TrkB did not show any deficits in the cochlea (data not shown), and only mild defects were found in the cochlea of the TrkbSHC/SHC point mutants [9]. In contrast, mice lacking both the PLCγ and the SHC docking sites of TrkB had an obvious reduction in radial fiber density in the cochlea (Fig. 2D, E) compared to control mice (Fig. 2F). These data strongly support the idea that the intracellular signalling of TrkB predominantly depends on these two docking sites, as the vestibular and cochlear phenotypes resulting from their mutation produces a virtually identical phenotype to that seen in Trkb null mutant animals.
TrkB/PLCγ signalling is necessary for fiber guidance in the sensory epithelium
Previously, counts of nerve fibers to a single vestibular sensory epithelium (the posterior canal cristae, PC) showed that TrkbSHC/SHC point mutant mice had reduced fiber growth with the remaining fibers exhibiting a reduction in nerve diameter and loss of myelin [9]. We carried out a similar analysis in TrkbPLC/PLC mutants by counting all nerve fibers to the PC sensory epithelium and found no differences in the number of nerve fibers apparent in juvenile or adult mutant mice, compared to controls (Fig. 3, 4. However, in the TrkbPLC/PLC mice there was a slight reduction in the degree of myelination (Fig. 4C), which was particularly apparent by transmission electron microscopy analysis (TEM) (Fig. 4E-F). These data suggest that the PLCγ signalling downstream of TrkB is not required for vestibular neuron fiber growth or maintenance, but rather functions in certain aspects of fiber maturation pertaining to their degree of myelination.
We next investigated nerve fiber innervation toward and within the vestibular sensory epithelia. The afferent fibers were targeted to a given sensory epithelia in both the TrkbPLC/PLC point mutant and the control animals at P0 and P8, showing no fibers outside the sensory epithelia (data not shown). Nerve fibers targeting the vestibular sensory epithelia were also observed in the TrkbSHC/SHC point mutants [9], and in the TrkbD/D point mutants (Fig. 2). Thus, nerve fiber targeting to the sensory epithelia mediated by BDNF [18] must use signalling mechanisms independent of the TrkB/SHC or PLCγ sites and/or factors other than BDNF/TrkB signaling such as NT3/TrkC.
Closer examination of the nerve fiber trajectories within the sensory epithelia showed aberrations in the TrkbPLC/PLC point mutant mice that were not found in the TrkbWT/WT control mice (Fig. 5) nor in the Trkb+/+ mice (data not shown). As early as P0, the TrkbWT/WT , like the Trkb+/+ , control mice showed obvious targeting of nerve fibers toward hair cells and already partial formation of calyces (Fig. 5C). Conversely, in TrkbPLC/PLC point mutants, nerve fibers extended for long distances along the perimeter of sensory epithelia (Fig. 5B, D). It is noteworthy that these fibers did not leave the sensory epithelia despite their obvious disorientation. In addition, at this stage these nerve fibers rarely engaged in the formation of calyces as compared to the TrkbWT/WT controls (Fig. 5C).
We further investigated calyx development at P8, a stage when most calyces are present [19, 20]. Labeling of afferent fibers from the cerebellum and the brainstem showed distinct groups of labelled fibers in control mice, apparently situated on either side of the striola region (Fig. 6A). Interestingly, TrkbPLC/PLC point mutant mice showed a comparable distribution of fibers labelled from the cerebellum and the brainstem as control mice (Fig. 6B), however, two major differences were immediately apparent even at low magnification. Firstly, many fibers in the TrkbPLC/PLC mutants crossed into the vicinity of the other fiber compartments and circled along the sensory epithelia perimeter, occasionally for several 100 μm (Fig. 6B, D). Secondly, despite the apparently normal fiber density, the calyces detected in the TrkbPLC/PLC point mutants were almost exclusively inside the striola region (Fig. 6D), whereas controls at P8 showed numerous calyces and good segregation of nerve fibers into discrete territories up to the perimeter of the utricle (Fig. 6C). Only a few fibers were near the margin of the sensory epithelium, and the fibers showed numerous branches consistent with their innervating type II hair cells [21]. In contrast, TrkbPLC/PLC point mutants displayed little fiber segregation, with both those at the margin and within the epithelium broadly overlapping (Fig. 6B, D). Similar effects were found in the other four vestibular epithelia (data not shown). Comparable results were also observed in older mutants (P24) and control mice (data not shown). This phenotype results in impaired balance in TrkbPLC/PLC mutants compared to control mice. The TrkbPLC/PLC mice showed a higher basal locomotor activity and displayed circling behavior, in either direction, compared to wild-type control mice (TrkbWT/WT) ) (Additional files 1 & 2).
Additional file 1: Locomotor activity of a TrkbPLC/PLC mouse. Movie demonstrating the circling behaviour of a TrkbPLC/PLC mouse in its home cage. Note the higher basal locomotor activity compared to controls (TrkbWT/WT , additional file 2) and that circling occurs in both directions. (MOV 16 MB)
Additional file 2: Locomotor activity of a TrkbWT/WTmouse. Movie demonstrating the locomotor behaviour of a TrkbWT/WT control mouse in its home cage. (MOV 16 MB)
Overall, these data suggest that a point mutation at the PLCγ site of TrkB causes disorientation of afferent fibers inside the vestibular sensory epithelia. In addition, nerve fibers either do not find type I hair cells or cannot engage in calyx formation, as a reduction in both the distribution and the degree of differentiation of calyces is apparent.
Ultra structural analysis confirms the inability of calyx formation in TrkbPLC/PLC point mutants
We next investigated the formation of calyces in histological sections of P24 and P100 control and TrkbPLC/PLC point mutants. Histological sections clearly revealed formation of calyces in all vestibular epithelia of control mice already at P24 (data not shown, and Fig. 7B, P100, utricle), as previously described [20, 22]. In contrast, TrkbPLC/PLC mutants showed little evidence of calyx formation in any vestibular sensory epithelium (Fig. 7C, P100, and data not shown). Likewise, electron microscopy (EM) analysis showed limited and mostly partial calyx formation throughout the vestibular epithelia of TrkbPLC/PLC mutants, in particular in the utricle (Fig. 7C, D), with the exception of an occasional calyx-like structure around the striola region of the utricle (Fig. 7E). In the canal cristae calyx formation was almost absent and only formation of boutons was apparent (Fig. 7F and insets Fig. 7H), indicating that the conditions for calyx formation inside and outside of the striola region and between sensory epithelia differ.
Trpc3 is overexpressed in vestibular organs of TrkbPLC/PLC mutants and may lead to the fiber misdirection
BDNF signalling has been shown to induce chemo-attractive turning of axonal growth cones in vitro [23]. Moreover, it has also been shown that BDNF-induced guidance of nerve growth cones in rat cerebellar granule cell cultures depends on the activation of signalling downstream of the PLCγ site of TrkB and Ca2+ elevation [11]. TRPC channels, in particular TRPC3/6, mediated the BDNF-induced elevation of Ca2+ in these cells, and were required for the nerve-growth cone turning induced by BDNF [11]. Because individual fibers inside the sensory epithelia showed impaired guidance in TrkbPLC/PLC mutants compared to controls (Fig. 6E-F), we decided to investigate the involvement of TRPC channels in fiber misdirection in the vestibular sensory epithelia of TrkbPLC/PLC mutants. We first examined the expression of Trpc1-7 by RT-PCR analyses using total RNA preparations from dissected vestibular ganglion tissue of young adult CF-1 male mice (4-5 weeks old). Positive control samples consisted of cDNA templates derived from a pool of total RNAs prepared from mouse embryonic day 15 (E15) brain and retina. As shown in Fig. 8A-B, Trpc1, 2s, 3, 4, and 7 are expressed in both vestibular tissue and E15 brain/retina. Since TRPC3/6, but not TRPC1, has been shown to be essential in the guidance of nerve growth cones by BDNF [11], we examined the expression of Trpc3 in the vestibular organ of TrkbPLC/PLC mutants and control (Trkb+/+ and the TrkbWT/WT ) mice. As shown in Fig. 8C, Trpc3 was overexpressed in the TrkbPLC/PLC mutants compared to controls (one-way ANOVA, p = 0.02). Moreover, mRNA levels of the neuronal marker neuron-specific enolase (NSE) were not significantly different between the TrkbPLC/PLC mutants and the control genotypes (Fig. 8D). We also anlysed Trpc4 channel expression by RT-PCR and found no differences between all genotypes analysed (data not shown).
KCNQ4 shows normal distribution in TrkbPLC/PLC mutants
KCNQ4 is an M-type K+ channel expressed in sensory hair cells of the inner ear and in the central auditory pathway. Previous work had suggested that Kcnq4 expression is restricted to the vestibular afferent calyx-like nerve endings ensheathing the type I hair cells [24]. However, an analysis of conditional Bdnf null mutant mice has revealed that type I hair cells express Kcnq4 in the absence of afferent and/or efferent innervations in the vestibular canal cristae [25]. Immunohistochemical analyses revealed no differences in the expression pattern of Kcnq4 in the canal cristae and the utricle of TrkbPLC/PLC and control mice at both P0 and P24 (Fig. 9A-D). Therefore, the distribution of type I hair cells is normal at a time (P0) when calyx formation is already affected in the absence of the PLCγ site of TrkB. By P24, the intensity of the immunohistochemical response of KCNQ4 in the tissue from TrkbPLC/PLC mutants compared to control tissue was reduced (Fig. 9A-D), suggesting that calyx formation does not regulate the expression pattern of KCNQ4, but its expression level in type I hair cells is influenced by proper innervation.
Discussion and Conclusions
The neurotrophin receptor tyrosine kinase TrkB is primarily known for its function during peripheral and central nervous system development [26]. In the CNS it is only recently emerging as a potent regulator of synaptic plasticity of the hippocampus as well as of other brain regions, and the signalling pathways and molecular mechanisms involved in these functions are beginning to be revealed [7, 27]. As for the peripheral nervous system, in order to dissect the signalling pathways involved in the predominant functions of TrkB receptors, such as neuronal survival as well as target innervation, we have chosen the vestibular organ as a model. We have previously reported that a point mutation at the SHC site of TrkB has limited effects on vestibular sensory neuron survival, indicating that TrkB receptors promote long-term survival of sensory neurons mainly in a SHC site-independent manner [8]. In contrast, target innervations of these sensory neurons were eventually lost in TrkBSHC mice; at P0 both afferent and efferent fibers were evident to all sensory epithelia, however by P8 they were greatly reduced [9], revealing that signalling through the SHC site of TrkB is required to maintain target innervations. However, no molecular data has been known until now concerning the growth of vestibular fibers toward specific vestibular hair cell types and, in the saccule and utricle, toward hair cells of different polarity. It was recently shown that fibers targeting hair cells of different polarity have distinct central projections [28], but the mechanisms underlying these features are unclear. We now show that in the absence of the PLCγ docking site of TrkB the apparent segregation of fibers from distinct central projection areas is partially disrupted. In particular, we show that while the growth of vestibular afferents to inner ear sensory epithelia are unaffected in TrkBPLC mutants, these fibers run along the peripheral margin of the sensory epithelia without taking sharp turns to innervate their specific hair cells as in control mice (Fig. 5,6). Thus, signalling elicited through the PLC-γ site of TrkB is involved in targeting processes inside the sensory epithelia. This is consistent with previous reports showing that targeted growth to sensory epithelia does not require neurotrophins [17, 29], does not require differentiated hair cells [18, 30], and can occur in the absence of sensory epithelia formation [31]. Moreover, it is the cooperation of both the SHC and the PLCγ docking sites of TrkB [13] that mediate the survival of the vestibular neurons, as the phenotype of the TrkBD mice with respect to the loss of these cells is essentially indistinguishable from that observed in Trkb null mice.
The TRPC3/6 calcium channels have been shown to mediate the BDNF-induced elevation of Ca2+ in rat cerebellar granule cell cultures and to be required for the nerve-growth cone turning induced by BDNF in these cells [11]. Here we show that TRPC3 is overexpressed in vestibular neurons of TrkBPLC point mutants. Overexpression of the Trpc3 channel has been reported to interfere with the cellular calcium homeostasis both in vitro and in vivo [32, 33], this could explain the misdirection of fibers observed in the TrkbPLC/PLC mutants. We show that calyx formation around type I hair cells in the vestibular sensory epithelia requires BDNF/TrkB signalling via the PLCγ docking site. In absence of this site, fibers show a limited ability to switch from growth to calyx formation.
Past research has convincingly shown that absence of any fibers at any time does not interfere with the maturation of recognizable type I and type II vestibular hair cells [20, 22, 34, 35] implying that this process is cell autonomous. Indeed, we show here that the development of morphologic characteristics of type I hair cells does not depend on the formation of a calyx. Instead, we suggest that calyx formation is mediated through the PLCγ activated signalling pathway(s) downstream of the TrkB receptor. In the absence of this pathway(s), fibers cannot be converted in fully developed calyces and form bouton terminals instead. Of note is the fact that type I hair cells receive efferent terminals (Fig. 7) which are virtually excluded from these cells by the developed calyx in wild type mice, and suggest that formation of a calyx is directly related to the exclusion of direct efferent innervations on type I hair cells. It remains unclear what exactly are the differences in the BDNF release from type I and type II hair cells, both of which are strongly positive for BDNF in embryos [2, 17, 36]. Closer examination of the amounts of BDNF expressed in postnatal type I and type II hair cells is necessary to understand whether there is a differential expression of BDNF in one of the two differentiating hair cell types thereby contributing to the transformation of boutons into calyces via the TrkB/PLCγ signalling activated pathway(s). It is entirely possible that the downregulation of Bdnf expression in postnatal ears [37–39] is different in the two hair cell types and thus provides the proper signalling for afferent transformation into calyces.
In summary, these data support the notion that the TrkB/PLCγ site activated pathway(s) mediate structural synaptic plasticity in the PNS. Indeed, our data showing the lack of proper development of afferent calyces on differentiated type I vestibular hair cells demonstrate clearly that BDNF signalling through the TrkB/PLCγ site activated pathway(s) is an essential component of this structural synaptic plasticity.
Methods
Mouse strains
Generation of the TrkB point mutant mice has been described previously [5, 13]. Briefly, the TrkBPLC and TrkBD mice were created using a cDNA "knock-in" approach [40]. Y816 (for the TrkBPLC mice) or Y515 and Y816 (for the TrkBD mice) were changed into phenylalanine in the mouse Trkb cDNA. Mutant Trkb cDNAs and wild-type Trkb cDNA (as a control for the strategy) were fused to the juxtamembrane exon of the mouse Trkb gene, and standard homologous recombination in ES cells (E14.1) was used to generate the targeted TrkBPLC, TrkbD and TrkbWT alleles.
Histology and neuron counts
Mice were fixed by cardiac perfusion using 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). The heads were decalcified in 5% formic acid in phosphate-buffered saline for 4 days, embedded in paraffin, and serial sectioned at 8 μm in the horizontal plane. For cellular counts, the neurons within the vestibular ganglia were visualized using 0.02% cresyl violet. Neurons with a clear nucleus were counted in every 5 sections (40 μm intervals). The total number of neurons in one vestibular ganglion was then calculated by dividing the cell count by the number of sections counted, and multiplying this value by the number of sections containing the vestibular ganglion. The total number of neurons obtained for each of the two vestibular ganglia per animal were averaged and the result was corrected for split nuclei using Abercrombie's correction formula [41]. In the figure, the values represent the means and standard errors for each genotype. Mean neuronal counts were compared using unpaired t-tests or using one-way ANOVA followed by a Tukey's Multiple Comparison test (GraphPad Prism). The number of animals used is as follows: TrkbWT/WT , n = 3; TrkbPLC/+ , n = 2; TrkbPLC/PLC , n = 5; TrkbD/D , n = 4; TrkbSHC/SHC , n = 2; Trkb-/- , n = 2.
Circling behavior analysis
Mice were allowed to roam freely in their home cage whilst videos were recorded using a digital camera. A representative sample of the locomotor activity is shown.
File Format: MOV. Size: 16 MB. Playing the movie requires QuickTime.
Immunohistochemistry
We used the rabbit KCNQ4 antibodies against KCNQ4 N- and C- termini followed by a goat anti-rabbit secondary antibody conjugated to Alexa Fluor 568 (Molecular Probes, Eugene, OR) as previously described [25]. Analyses using whole mount immunohistochemistry (IHC) and whole mount immunofluorescence (IF) were performed in the vestibular endorgans of mice at P0, P8 and P24. Due to low signal in the whole mount IF preparation of early postnatal vestibular tissue, we performed whole mount IHC and used the Vectastain® ABC Kit (Vector Laboratories, Inc, Burlingame, CA). Images were taken with a Zeiss LSM 510 Meta NLO confocal microscope. The number of animals used is as follows: TrkbWT/WT (P0 n = 3, P8 n = 3, P24 n = 2); TrkbPLC/PLC (P0 n = 7, P8 n = 3, P24 n = 2); TrkbD/D (P0 n = 2); TrkbSHC/SHC (P0 n = 2).
Lipophilic dye tracing
Mice at different stages were fixed by transcardiac perfusion with 4% PFA, and DiI lipophilic tracers (NV Maroon) were used to reveal afferent and efferent fibers of the inner ears as described [42]. Briefly, dyes were inserted into central target nuclei and left to diffuse to fill all profiles. Ears were subsequently dissected, mounted in glycerol and viewed with a Leica confocal microscope. The number of animals used for this experiment is as follows: TrkbWT/WT (P0 n = 3, P8 n = 5, P24 n = 2); TrkbPLC/+ (P8 n = 2, P24 n = 2); TrkbPLC/PLC (P0 n = 8, P8 n = 6, P24 n = 2); TrkbD/D (P0 n = 4); TrkbSHC/SHC (P8 n = 2).
Transmission Electron Microscopy and nerve diameter
Transmission Electron Microscopy (TEM) was preformed on osmicated ears, subdissected to allow serial sectioning of specific endorgans or nerves as previously described [9]. Thick sections were imaged in a compound microscope, while ultrathin sections were imaged using Hitachi or Jeol JEM 1230 TEM. Nerve numbers and diameters were counted and measured as previously described [9]; briefly, the diameter of the nerve to the posterior canal (PC) was measured using ImagePro software. The number of nerve fibers in the posterior vertical canal of P24, and P100 animals was determined by counting fibers on photographs taken at random throughout the nerve. The total number of fibers was then calculated using the measured area of the nerves. At least three sections at different levels of one canal were examined per animal. The number of animals used for this experiment is as follows: TrkbWT/WT (P0 n = 2, P8 n = 2, P24 n = 6, P100 n = 6); TrkbPLC/+ (P8 n = 1, P24 n = 4, P100 n = 6); TrkbPLC/PLC (P0 n = 3, P8 n = 3, P24 n = 10, P100 n = 8).
RNA isolation and Real-Time PCR
Trpc isoform expression in vestibular ganglion: total RNA was prepared according to [43]. RT-PCR reactions were performed according to [44] on total RNA from dissected vestibular ganglion of CF1 mouse inner ear. Positive controls consisted of cDNA templates derived from E15 mouse brain and retina. Negative controls consisted of no template or template without reverse transcriptase treatment. Gene-specific (gs) oligodeoxynucleotide primers were prepared for each of the Trpc genes (designed with the assistance of the Oligo 4.0 program, Natl. Biosciences, Plymouth, MN) as shown below (Table 1).
Analysis of Trpc3 channel in vestibular organ of Trkb point mutants: mice of each genotype at 4 months of age (for Trkb+/+ , n = 5 mice; for TrkbPLC/PLC , n = 6 mice and for TtrkbWT/WT , n = 3 mice) were killed by cervical dislocation, then decapitated and their heads bisected. After removing skin and fat from the specimen, vestibular ganglions were dissected bilaterally. Briefly, brain material was slowly lifted up with forceps in order to visualize the inner ear and afferent fibers under a dissecting microscope (Leica Microsystems, USA). Total RNA from vestibular ganglions was extracted with TRizol reagent (Invitrogen, Paisley, UK), according to the instructions of the manufacturer. For first strand cDNA synthesis, 1 μg of total RNA was reverse-transcribed with the Ready To Go T-Primed first strand kit (Amersham Biosciences, NJ, USA), according to the manufacturer's instructions. All the samples were finally brought to a final volume of 200 μl in DEPC-treated water. PCR primers for the murine Trpc3, Trpc4, homologs and internal controls ubiquitin and NSE (Table 2) were designed based on published sequences in GenBank (for Trpc3 and Trpc4 primers see Table 1).
Real-Time PCR analysis was performed using the LightCycler 480 system (Roche, Portugal). The PCR reactions were performed using SYBR Green Jumpstart Taq ReadyMix (Sigma-Aldrich, UK) using 384 plates (Roche, Portugal) in a total volume of 20 μl: 10 μl of 2X SYBR Green I, 5 μl of primers mastermix (1.2 μm), 2,5 μl cDNA sample and water to 20 μl. Each sample was analyzed in duplicate. Thermal cycling was initiated with activation of the Jumpstart Taq DNA Polymerase by denaturation during 10 min at 95°C, followed by 45 cycles of a 15 s melting step at 95°C, a 15 s annealing step at 62°C, and a 10 s elongation step at 72°C (all temperature transition rates at 20°C/s). After amplification for 45 cycles, at least 10 cycles beyond the beginning of the linear phase of amplification, samples were subjected to a melting curve analysis according to manufacturer's instructions in order to confirm the absence of nonspecific amplification products and primer-dimers. Melting curves for, Trpc3, Trpc4, ubiquitin, and NSE showed one specific peak.
mRNA quantitative analysis: the mRNA levels of the constitutively expressed housekeeping gene encoding ubiquitin were used as a control in all experiments and final results were compared with the mRNA levels of NSE. The relative changes in the mRNA levels of TRPC in murine vestibular ganglions were determined using the ΔΔCp method as previously reported [45, 46]. Accordingly, for each sample the "Crossing point" (Cp) values given by the LightCycler system II software, for each target gene, were subtracted by the respective Cp value determined for the ubiquitin gene for the same sample and condition (ΔCp). This allows normalizing changes in target gene expression. Afterwards, the ΔCp values were subtracted by the respective values of the control for the target gene giving ΔΔCp. The derivation to the formula 2-(ΔΔCp) sets each control at the unity since ΔΔCp (control) = 0.Statistical analysis was performed using one-way ANOVA followed by the Student's t test.
Authorization for the use of experimental animals
EMBL-Monterotondo Animal Ethics Committee approved all animal procedures. We further confirm that all animal experiments conform to Italian and European regulatory standards.
Abbreviations
- AKT:
-
v-akt murine thymoma viral oncogene homolog
- BDNF:
-
brain-derived neurotrophic factor
- CamKII:
-
calcium/calmodulin-dependent kinase II
- DiI:
-
1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate
- FRS-2:
-
FGF receptor substrate 2
- IF:
-
immunofluorescence
- IHC:
-
immunohistochemistry
- KCNQ4:
-
potassium voltage-gated channel subfamily KQT member 4
- LTP:
-
Long-term potentiation
- MAPK:
-
Ras/mitogen activated protein kinase
- NSE:
-
neuron-specfic enolase
- PI3K:
-
phosphoinositide 3-kinase
- PLCγ:
-
phospholipase Cγ
- PC:
-
posterior canal crista
- SHC:
-
Src homology 2 domain-containing-transforming protein
- TrkB/Ntk2:
-
neurotrophin tyrosine kinase receptor B
- Trkb -/- :
-
mice carrying the germ-line null mutation for Trkb
- TrkBD:
-
mice with germ-line Y515F and Y816F point mutations in TrkB
- TrkBPLC:
-
mice with a germ-line Y816F point mutation in TrkB
- TrkBSHC:
-
mice with a germ-line Y515F point mutation in TrkB
- TrkBWT:
-
control mouse line for the TrkBPLC mutant mice
- TRPC3:
-
Transient receptor potential cation channel, subfamily C, member 3.
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Acknowledgements
The Authors would like to thank Emerald Perlas for technical assistance. This work was supported in part by a grant from the NIH/NIDCD (RO1DC005590) to BF, and a grant from the NIH/NIDCD (RO1DC009418) to LM.
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CS and BF planned and performed most of the experiments and, together with LM, drafted the manuscript. AB, KB and SR-S carried out the TrpC investigation and imaging of adult sensory epithelia. JH performed the behavioral analysis and imaging of the vestibular organs. LM conceived of the study, contributed to the experimental plans, provided theoretical input and supervision and wrote the manuscript. All authors read and approved the manuscript.
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Sciarretta, C., Fritzsch, B., Beisel, K. et al. PLCγ-activated signalling is essential for TrkB mediated sensory neuron structural plasticity. BMC Dev Biol 10, 103 (2010). https://doi.org/10.1186/1471-213X-10-103
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DOI: https://doi.org/10.1186/1471-213X-10-103