Abstract
A weighted biological sequence is a string in which a set of characters may appear at each position with respective probabilities of occurrence. We attempt to locate all the tandem repeats in a weighted sequence. A repeated substring is called a tandem repeat if each occurrence of the substring is directly adjacent to each other. By introducing the idea of equivalence classes in weighted sequences, we identify the tandem repeats of every possible length using an iterative partitioning technique. We also present the algorithm for recording the tandem repeats, and prove that the problem can be solved in O(n^{2}) time.
Introduction
A weighted biological sequence, called for short a weighted sequence, is a special string that allows a set of characters to occur at each position of the sequence with respective probability, instead of a fixed single character occurring in a normal string. It can be viewed as a compressed version of multiple alignment which shows strength in extracting and representing the conserved commonalities of a set of sequences.
Weighted sequences are apt at summarizing poorly defined short sequences, e.g. transcription factor binding sites, the profiles of protein families and complete chromosome sequences[1]. With this model, one can attempt to locate the motifs of biological importance, to estimate the binding energy of the proteins, even to infer the evolutionary homology. It thus exhibits theoretical and practical significance to design powerful algorithms on weighted sequences in proteins.
This paper concentrates on locating those tandem repeats in a weighted sequence. Tandem repeats occur in a string when a substring is repeated for two or more times and each repetition is directly adjacent to each other. For example, The substring ATT occurs in the string X = CATT ATT ATTG for three times, and each occurrence of ATT is consecutive, one after the other. Then ATT is a tandem repeat of length 3 of X.
The motivation for investigating tandem repeats in weighted sequences comes from the striking feature of DNA that vast quantities of tandemly repetitive segments occur in the genome, with high proportion of more than 50 percent in fact[2]. Some examples are microsatellite, minisatellite, and satellite DNA.
It should be noticed that tandem repeats are not redundant information, but of either functional or evolutionary significance [3]. For instance, tandem repeats frequently occur within or in the proximity of genes, i.e., either in the untranslated regions up and downstream of open reading frames, within introns, or in coding regions[4]. Recent evidence supports that tandem repeats in these regions can play a significant role in regulating gene expression and modulating gene function[5]. Thus it is of great biological interest to locate tandem repeats in biological DNA sequences and proteins.
It has been an effort for a long time to identify special areas in a biological sequence by their structure. Large amount of work has been done to find all tandem repeats in nonweighted strings. Technically, these solutions can be divided into two main categories. One employed traditional string comparison and searching method, where the most famous algorithms were Crochemore's partioning[6] and LZ decomposition[7], with time complexity O(n log n) respectively. The other computed tandem repeats by constructing suffix tree and suffix array. Although needing extra memory, these algorithms can also reach O(n log n) time by limiting the number of output[8–12].
However, relatively less work has been studied in weighted sequences circumstance. Iliopoulos et al.[13, 14] were the first to touch this field, and extract repeats and other types of repetitive motifs in weighted sequences by constructing weighted suffix tree. Weighted suffix tree was built simulating suffix tree, with the distinction that the weight of each substring should be considered. This directly led to a big size and its strong dependence on the presence probability of the weighed suffix tree. Another solution[15, 16] used the partitioning technique based on KMR algorithm to find tandem repeats of length d in O(n log d) time. But they did not give efficient algorithm for computing the tandem repeats of all lengths.
On the other hand, a lot of recent results of studies on identifying hot spots in proteins enlightened us. Huang et al.[17] firstly utilized the support vector machine(SVM) classifier based upon the hydropathy blocks to classify protein sequences. Then Xia et al.[18] used support vector machine (SVM) to predict hot spot residues in protein interfaces. Selecting nine individual features from 62 features, they developed a new ensemble classifier APIS to further improve the prediction accuracy. You et al.[19] developed a robust manifold embedding technique for assessing the reliability of interactions and predicting new interactions, which was reinterpreted into the problem of measuring similarity between points of its metric space after transforming a given PPI network into a low dimensional metric space using manifold embedding based on isometric feature mapping. Zheng et al.[20] employed independent component analysis for gene selection, then introduced gene selection and explicitly enforcing sparseness into nonnegative matrix factorization for tumor clustering. Wang et al.[21] proposed a novel tumor classification method based on correlation filters other than the model to identify the overall pattern of tumor subtype hidden in genes.
The paper focuses on finding tandem repeats of all length in a given weighted sequence in proteins. The paper is organized as follows. In the next section we give the necessary theoretical preliminaries used, then introduce the alltandemrepeats problem and explains why Crochemore's partitioning algorithm cannot be adapted to weighted sequences. After that, we present our algorithm for computing all the tandem repeats in weighted sequences, and give experimental results to verify the algorithm's performance. Finally we conclude and discuss our research interest.
Preliminaries
A biological sequence used throughout the paper is a string either over the 4character DNA alphabet Σ ={A,C,G,T} of nucleotides or the 20character alphabet of amino acids. Assume that readers have essential knowledge of the basic concepts of strings, now we extend parts of them to weighted sequences. Formally speaking:
Definition 1 Let an alphabet be Σ = {σ_{1}, σ_{2}, . . . , σ_{l}}. A weighted sequence X over Σ, denoted by X [1, n] = X[1]X [2] . . . X[n], is a sequence of n sets X[i] for 1 ≤ i ≤ n, such that:
Each X[i] is a set of couples (σ_{ j }, π_{ i } (σ_{ j })), where π_{ i }(σ_{ j }) is the nonnegative weight of σ_{ j } at position i, representing the probability of having character σ_{ j } at position i of X.
Let X be a weighted sequence of length n, σ be a character in Σ. We say that σ occurs at position i of X if and only if π_{ i }(σ) > 0, written as σ ∈ X[i]. A nonempty nonweighted string f[1,m] (m ∈ [1, n]) occurs at position i of X if and only if position i + j − 1 is an occurrence of the character f [j] in X, for all 1 ≤ j ≤ m. Then f is said to be a factor of X, and i is an occurrence of f in X.
The probability of the presence of f at position i of X is called the weight of f at i, written as π_{ i }(f), which can be obtained by using different weight measures. We exploit the one in common use, called the cumulative weight, defined as the product of the weight of the character at every position of f:{\pi}_{i}\left(f\right)={\prod}_{j=1}^{m}{{\pi}_{i}{}_{+j}}_{1}\left(f\left[j\right]\right).
Considering the following weighted sequence of length 5:
the weight of f = GAT at position 2 of X is: π_{2}(f) = 1 × 0.6 × 0.25 = 0.15. That is, GAT occurs at position 2 of X with probability 0.15. Note that for clarity, we employ a simplified vertical representation method for a weighted sequence, where the probability 1 can be ignored by simply remaining the character with probability 1.
A factor f of a weighted sequence X is called a repeat in X if there exist at least two distinct positions of X that are occurrences of f in X. As a special case of repeat, tandem repeats can be formally defined as follows.
Definition 2 A factor f of length p of a weighted sequence X is called a tandem repeat in X if there exists a triple (i, f, l) such that for each 0 ≤ j <l − 1, position i + jp is an occurrence of the factor f in X.
It is easy to see that, the difficulty for locating the tandem repeats in weighted sequences arises from uncertainties of weighted sequences. Firstly, different characters might occur at the same position, which yields multiple factors of equal length at each position of the weighted sequence. Secondly, as each character occurs at one position with respective probability, the corresponding factors produced also have different presence probabilities, thus the weight of each appearance of a factor f can be highly different.
As scientists pay more attention to the pieces with high probabilities in DNA sequences, we fix a constant threshold for the presence probability of the motif, that is, only those occurrences with probability not less than this threshold are counted.
Definition 3 Let f be a factor of length d of a weighted sequence X that occurs at position i, a real constant threshold k ≥ 1. We say that f is a real factor of X if and only if the weight (probability) of f at i, π_{ i }(f), is at least \frac{1}{k}. Exactly, {\prod}_{j=1}^{d}{{\pi}_{i}{}_{+j}}_{1}\left(f\left[j\right]\right)\ge \frac{1}{k}.
In the above example (1), set 1/k = 0.3, then AGA is a real factor of X that occurs at position 1 since π_{1}(AGA) = 0.5 × 1 × 0.6 = 0.3 ≥ 0.3, while CAC is not a real factor of X at position 3 since π_{3}(CAC) = 0.1 < 0.3.
The alltandemrepeats problem
Now we introduce the alltandemrepeats problem in weighted sequences as below:
Problem 1 Given a weighted sequence X[1, n] and a real constant k ≥ 1, the alltandemrepeats problem identifies the set S of all triples (i, f, l), where 1 ≤  f  ≤ n/2 and f is a real factor of X.
Our algorithm for picking all the tandem repeats is based on the following idea of equivalence relation on positions of a string:
Definition 4 Given a string x of length n over Σ, an integer p ∈{1, 2, . . . , n}, S be a set of positions of x: {1, 2, . . . , n − p + 1}, then E_{ p } is defined to be an equivalence relation on S such that: for two positions i, j ∈ S, (i, j) ∈ E_{ p } if x[i, i + p − 1] = x[j, j + p − 1].
In the following context, a nonempty substring of x of length p is called a psubstring of x. Clearly, two positions i and j of x are said to be pequivalent when two psubstrings starting at i and j in x are identical. Although this definition is defined on nonweighted strings, it can also be extended to weighted sequences. Before presenting our algorithm, we first introduce Crochemore's partitioning algorithm[6] for computing tandem repeats in nonweighted sequences. The algorithm employs the following idea of equivalence class and partition.
Definition 5 Consider the substring w = x[i, i + p − 1] for i ∈ S. The set of all positions of x that are related to i, i.e, {j(i, j) ∈ E_{ p }, j ∈ S}, is called the equivalence class of i, or alternatively, the equivalence class associated with w, denoted by C_{ w }.
Definition 6 Let S_{1}, S_{2}, . . . , S_{ r } be nonempty subsets of S, we say that {S_{1}, S_{2}, . . . , S_{ r }} is a partition of S if:

(i)
S = S_{1} ∪ S_{2} ∪ . . . ∪ S_{ r }

(ii)
S_{ i }∩ S_{ j }= Ø for 1 ≤ i, j ≤ r and i ≠ j.
For an equivalence relation E_{ p } on a set S, all the equivalence classes of E_{ p }, called E_{ p }classes, compose a partition of S, since every element of S falls into exactly one E_{ p }class. We also say that S is partitioned into a family of E_{ p }classes. In this sense, partitions and equivalence relations are the same.
It is obvious that each E_{ p }class of cardinality not less than two records the occurrences of a repetitive psubstring of x. Hence, the problem of computing all the repeated psubstrings of x can be rephrased as finding the partition of E_{ p }.
Observe that E_{ p }_{+1} is a refinement of E_{ p } by excluding the position n − p + 1. Thus the equivalence relations can be iteratively constructed by starting with E_{1}, then successively building E_{2}, E_{3}, etc., until E_{ L } such that each E_{ L }class is a singleton who refers to a set that consists of only one element. Crochemore efficiently executed this iterative computation and located all the tandem repeats in x in O(nlogn) time by introducing the following ideas:
 Smallclasses: Consider the refinement from E_{ p } to E_{ p }_{+1} . Assume that an E_{ p }class C is partitioned into r E_{ p }_{+1}classes, we call the one of maximal size a big class of C, and the other r − 1's are small classes.
 Smallerhalf trick : The trick depends on the following Lemma:
Lemma 1 Let × be a string of length n, p ∈ {1, 2, . . . , n}, i, j ∈ {1, 2, ... , n − p}. Then:
Therefore, instead of partitioning all E_{ p }classes at stage p, the algorithm simply examines each small E_{ p }class SC and partitions those related classes RC such that {RC i ∈ RC and i + 1 ∈ SC}. Simply speaking, for any E_{ p }class C, only the positions that will be transferred into small E_{ p }_{+1}classes are assigned new indexes, while the big E_{ p }_{+1}class directly inherits the index of C.
The running time of this algorithm is proportional to the union of small classes. By definition, all the E_{1}classes are small, with cardinality less than n. As each small E_{ p }_{+1}class has the size not greater than half of the cardinality of its corresponding E_{ p }class, a position cannot belong to a small class more than logn times. Therefore, the partitioning algorithm takes O(nlogn) time for a string of length n.
Although proved to be optimal, this algorithm cannot conform to a weighted sequence X due to the following reasons:
1. Multiple distinct characters may occur at the same one position of a weighted sequence. In this case, a position may goes to more than one equivalence classes associated with different substrings of the same length, thus the smallerhalf trick makes no sense.
2. In weighted sequence circumstance, the presence probability of any factor should not be ignored as it is restricted by the probability threshold.
Our algorithm
As we stated above, Crochemore's algorithm cannot be directly used in weighted sequence, but it enlightens us to borrow the idea of partitioning. By improving the method for computing repeated patterns in weighted sequences we proposed in [22], we first simulate the definition for E_{ p }classes of nonweighted strings, and give the corresponding weighted version:
Definition 7 Consider a factor f of length p in a weighted sequence X[1, n]. An E_{ p }class associated with f is the set C_{ f }(p) of all positionprobability pairs, denoted by (i, π_{ i }(f)), such that f occurs at position i with probability π_{ i }(f) ≥ 1/k.
C_{ f }(p) is an ordered list that contains all the positions of X where f occurs. Note that only the occurrences of those real factors are considered. For this reason, the probability of each appearance of a factor should be recorded and kept for the next iteration.
Although tandem repeats are special cases of repeats in weighted sequences, the following facts draw a distinction between the algorithms for computing tandem repeats and the repeats we proposed before.
Fact 1 The occurrences of a tandem repeat are not overlapping.
Fact 2 If a factor f is a tandem repeat of X, any consecutive alignment of f should not be reported as a tandem repeat again.
For instance, a string AT AT AT AT will report a tandem repeat (1, AT, 4), not (1, AT AT, 2). According to the above facts, tandem repeats can be timely filtered during the construction of equivalence classes.
Note that in this construction process, a position i is allowed to go to several but no more than Σ different E_{ p }classes, due to the uncertainty of weighted sequences. Though, we follow to use the notion "partition" to describe the process of building E_{ p }classes from E_{ p }_{−1}classes, which can be computed based upon the following corollary:
Corollary 1 Let p ∈ {1, 2, . . . , n}, i, j ∈ {1, 2, ... , n − p}. Then:
((i, π_{ i }(f)), (j, π_{ j }(f))) ∈ C_{ f }(p) iff ((i, π_{ i }(f'), \left(j,{\pi}_{j}\left({f}^{\prime}\right)\right)\in {C}_{{f}^{\prime}}\left(p1\right)and ((i + p − 1, π_{ i }_{+}_{ p }_{−1}(σ)), (j + p − 1, π_{ j }_{+}_{ p }_{−1}(σ))) ∈ C_{ σ }(1)
where σ ∈ Σ, f and f'are two factors of length p and p − 1 respectively, such that f = f'σ and π_{ i }(f) ≥ 1/k, π_{ j }(f) ≥ 1/k.
Our algorithm for picking all the tandem repeats of X then operates as follows:
1. "Partition" all the n positions of X to build E_{1} and detect all the tandem repeats of length 1: For every character σ ∈ Σ, create a class C_{ σ }(1) that is an ordered list of couples (i, π_{ i }(σ)), where i is an occurrence of σ in X with probability not less than 1/k. Each class composed of more than one element forms E_{1}. Those C_{ σ }(1)s in which the distance between two or more adjacent position i is 1 report the tandem repeats of length 1.
2. Iteratively compute E_{ p }classes from E_{ p }_{−1}classes using the above corollary for p ≥ 2, and find all the tandem repeats of length p: Take each class C(p − 1) of E_{ p }_{−1}, partition C(p − 1) so that any two positions i, j ∈ C(p − 1) go to the same E_{ p }class if positions i + p − 1, j + p − 1 belongs to a same E_{1}class, and this E_{ p }class represents a real factor of X.
3. For each E_{ p }class C(p) partitioned by C(p − 1), test if the factor associated with C(p) is a tandem repeat of X: If the cardinality of C(p) is at least two and any distance between two or more adjacent positions in C(p) equals p, add the corresponding triple into the tandem repeat set \mathcal{S}. Eliminate those C(p)s who are singletons, and keep the rest to proceed the iterative computation at stage p + 1.
4. The computation stops at stage L, once no new E_{ L }_{+1}classes can be created or each E_{ L }class is a singleton.
Algorithm 1 Compute all the tandem repeats of a weighted sequence
Input: a weighted sequence X[1, n], k ≥ 2 ∈ R
Output: all the tandem repeats of X
1: Algorithm ComputeTandemRepeats(X, k)
2: for i ← 1 to n do
3: l ← 0
4: for j ← 1 to Σ do
5: for each σ_{ j }∈ X[i] do
6: while {{\pi}_{i}}_{+l}\left({\sigma}_{j}\right)\ge \frac{1}{k}do
7: add(i + l, π_{ i }+l(σ_{j})) to {C}_{{\sigma}_{j}}(1)
8: l ← l +1
9: if l > 1 then
10: \mathcal{S}\leftarrow \mathcal{S}\cup \left(i,{\sigma}_{j},l\right)
11: p ← 1
12: while p\le \frac{n}{2}and there is a nonsingleton class C(p − 1) of E_{ p }_{−1} or E_{ p }_{−1}≠ Ø do
13: (C_{ f }(p − 1), f) ← extract a pair from E_{ p }_{−1} list
14: SUB ← CreateEquivClass(C_{ f }(p − 1), f)
15: p ← p + 1
16: add SUB to E_{ p }
We use a doubly linked list to store each equivalence class, which needs O(n) space for a boundedsize alphabet. The computation for tandem repeats is demonstrated as Algorithm 1, which repeatedly calls function CreateEquivClass. Algorithm 2 depicts the procedure to construct all possible E_{ p }classes from a certain E_{ p }_{−1}class, and report those tandem repeats of length p. It is easy to see that Algorithm 1 takes O(n^{2}) time for a constantsize alphabet, since each refinement of E_{ p } from E_{ p }_{−1} costs linear time, and there are O(n) stages in total. The running time of Algorithm 2 is proportional to the size of the given E_{ p }_{−1}class, since tandem repeats of length p are reported along with the partitioning of the given E_{ p− }_{1}class. Taking all the E_{ p }_{−1}classes into account, stage p requires O(n) time and O(n) extra space. Thus the overall time complexity of finding all tandem repeats of every possible length amounts to O(n^{2}).
Algorithm 2 Identify tandem repeats of length p
Input: An E_{ p }_{−1}pair: class C_{ f }(p − 1), a factor f corresponding to C_{p−1}
Output: All the E_{ p }pairs derived from the input
1: Function CreateEquivClass(C_{ f } (p − 1), f)
2: for each (i, π_{ i } (f)) ∈ C_{ f }(p − 1) do
3: l ← 0
4: for each σ_{ j }∈ X [i + p − 1] do
5: f_{ j }← fσ_{ j }
6: π_{ i } (f_{ j }) ← π_{ i } (f) × π_{ i } + p − 1(σ_{ j })
7: while {\pi}_{+l}\left({}_{j}\right)\ge \frac{1}{k}do
8: add(i + l, π_{ i }+l(j)) to {C}_{{f}_{j}}(p)
9: l ← l +1
10: if l > 1 then
11: \mathcal{S}\leftarrow \mathcal{S}\cup \left(i,{f}_{j},l\right)
12: for each j do
13: if {C}_{{f}_{j}} (p) = 1 then
14: delete {C}_{{f}_{j}} (p)
15: else
16: add \left({C}_{{f}_{j}}\left(p\right),{f}_{j}\right)\phantom{\rule{0.3em}{0ex}}{E}_{p}
17: return E_{ p }
Theorem 1 The alltandemrepeats problem can be solved in O(n^{2}) time.
Experimental results
To verify the running time of our algorithm, we implemented the algorithm, programmed in C++, for locating all the tandem repeats in a given weighted sequence. The experiment environment is a Intel Core2 Duo CPU P8700 2.5GHz system, with 2GB of RAM, under the Microsoft Windows XP operating system (SP2).
In our experiments, the family of SR (serine/arginine rich) proteins SC35 across species and alleles was used. We transformed the alignment of the sequences [23] to a weighted sequence as the input data. Firstly, we fixed the presence probability threshold to be a small constant, then simply tested the performance of the algorithm with respect to the size of the weighted sequence, denoted by n. In this case, set the constant 1/k = 0.01. Figure 1 demonstrates the running time curve of our algorithm with respect to n. It is easily observed that, the algorithm runs in O(n^{2}) time as expected.
As we stated before, our algorithms is heavily dependent on the presence probability. We then fixed the size of the input weighted sequence to be 400, and executed our algorithm considering different presence probabilities. Figure 2 gives the time consumption of the the algorithm with respect to the presence probability 1/k. Clearly, the running time grows exponentially as the probability threshold gets smaller.
Conclusions
The paper investigated the tandem repeats arisen in weighted sequences. As opposed to the nonweighted version, the uncertainty of weighted sequences and the presence probability of every character in the sequence must be considered. We devised efficient algorithm for identify all the tandem repeats in a weighted sequence, which operates in O(n^{2}) time.
Note that if Σ are sufficiently large, the total number of repeats might be very huge. In the worst case, i.e. each character of Σ appears at every position of the weighted sequence, the total number of repeats of a weighted sequence can be exponential, that is O(Σ^{n}). This fact of considering equivalenceclasses of positions seems to lead to a quadratic algorithm. If Σ is relatively small, and the number of weighted positions in the weighted sequence is bounded, the algorithm appears to be running in O(n^{2}) time as expected.
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Acknowledgements
This work is supported by National Key Technology R&D Program(Grant No: 2012BAI34B01).
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This article has been published as part of BMC Bioinformatics Volume 14 Supplement 8, 2013: Proceedings of the 2012 International Conference on Intelligent Computing (ICIC 2012). The full contents of the supplement are available online at http://www.biomedcentral.com/bmcbioinformatics/supplements/14/S8.
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Zhang, H., Guo, Q. & Iliopoulos, C.S. Locating tandem repeats in weighted sequences in proteins. BMC Bioinformatics 14 (Suppl 8), S2 (2013). https://doi.org/10.1186/1471210514S8S2
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DOI: https://doi.org/10.1186/1471210514S8S2