After the publication of this work [1] it was brought to our attention that the concentrations of the reagents listed for the cell lysis buffer are those of the original stock solutions and not the required final concentrations. The correct final concentrations for this buffer are as follows:
HEPES 50 mM
NaCl 100 mM
NaF 10 mM
EDTA 5 mM
Na3VO4 0.5 mM
NEM 2 mM
Triton 0.1%
Complete protease inhibitors (Roche).
We regret any inconvenience that this inaccuracy may have caused, and thank Dr. Bräuer for bringing it to our attention.
References
Burnett C, Makridou P, Hewlett L, Howard K: Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity. BMC Biochemistry. 2004, 5: 2-10.1186/1471-2091-5-2.
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The online version of the original article can be found at 10.1186/1471-2091-5-2
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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Burnett, C., Makridou, P., Hewlett, L. et al. Erratum to: Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity. BMC Biochem 6, 3 (2005). https://doi.org/10.1186/1471-2091-6-3
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DOI: https://doi.org/10.1186/1471-2091-6-3