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Reciprocal Communication Between Endometrial Stromal Cells and Macrophages

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Abstract

This study tested the hypothesis that reciprocal communication occurs between macrophages and cultured human endometrial stromal cells and that this communication may contribute to the pathology of endometriosis. An endometrial stromal cell line (telomerase-immortalized human endometrial stromal cell [T-HESC]) was treated with macrophage-conditioned medium (CM) ± estradiol + progesterone. Macrophages were treated without or with T-HESC CM. DNA microarray identified 716 differentially expressed genes in T-HESCs in response to factors secreted by macrophages. Upregulated genes in T-HESC included interleukin 8 (IL-8)/chemokine (C-X-C motif) ligand 8 (CXCL8), matrix metalloproteinase 3 (MMP3), phospholamban, cysteine-rich angiogenic inducer 61 (CYR61), connective tissue growth factor (CTGF), tenascin C, and nicotinamide N-methyltransferase (NNMT), whereas integrin α-6 was downregulated. In contrast, 15 named genes were differentially expressed in macrophages in response to factors secreted by endometrial stromal cells. The data document reciprocal communication between macrophages and endometrial stromal cells and suggest that interaction with macrophages stimulates the expression of genes in endometrial stromal cells that may support the establishment of endometriosis.

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Authors and Affiliations

  1. Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, 414 E Clark St, 57069, Vermillion, SD, USA

    Kathleen M. Eyster PhD, Emily Winterton BS, Olga Klinkova MD, Donis Drappeau MS & Connie J. Mark-Kappeler PhD

  2. Department of Obstetrics and Gynecology, Sanford School of Medicine of the University of South Dakota, Sioux Falls, SD, USA

    Kathleen M. Eyster PhD & Keith A. Hansen MD

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  1. Kathleen M. Eyster PhD
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  2. Keith A. Hansen MD
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  6. Connie J. Mark-Kappeler PhD
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Correspondence to Kathleen M. Eyster PhD.

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Eyster, K.M., Hansen, K.A., Winterton, E. et al. Reciprocal Communication Between Endometrial Stromal Cells and Macrophages. Reprod. Sci. 17, 809–822 (2010). https://doi.org/10.1177/1933719110371854

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