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Evaluation of Matrix Metalloproteinase—2 Expression in Cervical Carcinogenesis Using Tissue Array and Integrated Optical Density for Immunoreactivity

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Abstract

To correlate matrix metalloproteinase—2 (MMP-2) expression with cervical carcinogenesis, the authors use reverse-transcription polymerase chain reaction to detect MMP-2 mRNA expression in 10 cervical squamous cell carcinoma (SCC), 10 high-grade cervical intraepithelial neoplasia (CIN), and 10 normal tissues. They further detect MMP-2 immunoreactivity of 24 tissue cores in each SCC, high-and low-grade CINs, and a healthy subgroup on a tissue array using integrated optical density (IOD) for number and intensity of stained cells in 345 × 345 pixels. They found the mRNA expression of MMP-2 to be higher in most SCCs (9/10 samples) and high-grade CINs (7/10 samples) but lower in normal tissues. The IOD of MMP-2 was significantly higher in high-grade CIN than in normal and low-grade CIN tissue cores (P <.001 for both) and significantly higher in SCC than in high-grade CIN tissue cores (P <.001). The results show that MMP-2 upregulation confers on tumor cells the ability to degrade the subepithelial basement membrane and subsequently invade the cervix.

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Author notes

  1. L-YL and P-HW contributed equally as the corresponding authors.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Chung Shan Medical University, Taichung, Taiwan

    Yi-Torng Tee PhD, Gin-Den Chen PhD, Horng-Jyh Tsai MD, PhD, Long-Yau Lin MD, PhD & Po-Hui Wang MD, PhD

  2. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Yi-Torng Tee PhD, Chih-Ping Han MD, PhD, Shun-Fa Yang PhD, Horng-Jyh Tsai MD, PhD, Long-Yau Lin MD, PhD & Po-Hui Wang MD, PhD

  3. School of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Gin-Den Chen PhD, Horng-Jyh Tsai MD, PhD, Long-Yau Lin MD, PhD & Po-Hui Wang MD, PhD

  4. Clinical Research Center, Chung Shan Medical University, Taichung, Taiwan

    Chih-Ping Han MD, PhD

  5. Department of Pathology, Chung Shan Medical University, Taichung, Taiwan

    Chih-Ping Han MD, PhD

  6. Institute of Medical and Molecular Toxicology, Chung Shan Medical University, Taichung, Taiwan

    Jiunn-Liang Ko PhD

  7. Department of Family Medicine, Chung Shan Medical University, Taichung, Taiwan

    Shiuan-Chih Chen MD, PhD

Authors
  1. Yi-Torng Tee PhD
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  2. Chih-Ping Han MD, PhD
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  9. Po-Hui Wang MD, PhD
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Corresponding author

Correspondence to Po-Hui Wang MD, PhD.

Additional information

We acknowledge grant support from Chung Shan Medical University (CSMU 95-OM-B-014). We also acknowledge Yi-Chieh Chen and Ken Hsu, who provided kind assistance for the construction and immunohistochemical staining of the cervical tissue array.

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Tee, YT., Han, CP., Ko, JL. et al. Evaluation of Matrix Metalloproteinase—2 Expression in Cervical Carcinogenesis Using Tissue Array and Integrated Optical Density for Immunoreactivity. Reprod. Sci. 14, 719–726 (2007). https://doi.org/10.1177/1933719107307537

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  • DOI: https://doi.org/10.1177/1933719107307537

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