Skip to main content
SpringerLink
Log in

The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer

  • Protocol
  • First Online:
Cervical Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1249))

Abstract

Cervical cancer is the third most common cancer affecting women worldwide. It is characterized by chromosomal aberrations and alteration in the expression levels of many cell cycle regulatory proteins, driven primarily by transforming human papillomavirus (HPV) infection. MYBL2 is a member of the MYB proto-oncogene family that encodes DNA binding proteins. These proteins are involved in cell proliferation and control of cellular differentiation. We have previously demonstrated the utility of MYBL2 as a putative biomarker for cervical pre-cancer and cancer. In this chapter we describe the methodological approach for testing MYBL2 protein expression in tissue biopsies from cases of cervical intraepithelial neoplasia (CIN) and cervical cancer, using immunohistochemistry techniques on the automated immunostaining platform, the Ventana BenchMark LT. The protocol outlines the various steps in the procedure from cutting tissue sections, antibody optimization, antigen retrieval, immunostaining, and histological review.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. International Agency for Research on Cancer (2008) GLOBOCAN

    Google Scholar 

  2. Walboomers JM, Jacobs MV, Manos M et al (1999) Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol 189(1):12–19, PMID: 10451482

    Article  PubMed  CAS  Google Scholar 

  3. Duensing S, Munger K (2003) Human papillomavirus 16 E7 oncoprotein can induce abnormal centrosome duplication through a mechanism independent of inactivation of retinoblastoma protein family members. J Virol 77(22):12331–12335, PMID: 14581569

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  4. Duensing S, Munger K (2004) Mechanisms of genomic instability in human cancer: insights from studies with human papillomavirus oncoproteins. Int J Cancer 109(2):157–162, PMID: 14750163

    Article  PubMed  CAS  Google Scholar 

  5. Narisawa-Saito M, Kiyono T (2007) Basic mechanisms of high-risk human papillomavirus-induced carcinogenesis: roles of E6 and E7 proteins. Cancer Sci 98(10):1505–1511, PMID: 17645777

    Article  PubMed  CAS  Google Scholar 

  6. Lyon J, Robinson C, Watson R (1994) The role of Myb proteins in normal and neoplastic cell proliferation. Crit Rev Oncog 5(4):373–388, PMID: 7711114

    Article  PubMed  CAS  Google Scholar 

  7. Ansieau S, Kowenz-Leutz E, Dechend R, Leutz A (1997) B-MYB a repressed trans-activating protein. J Mol Med 75:815–19, PMID: 9428611

    Article  PubMed  CAS  Google Scholar 

  8. Bessa M, Joaquin M, Tavner F, Saville MK, Watson RJ (2001) Regulation of the cell cycle by B-Myb. Blood Cells Mol Dis 27(2):416–21, PMID: 11259164

    Article  PubMed  CAS  Google Scholar 

  9. Lam EW, Robinson C, Watson RJ (1992) Characterization and cell cycle-regulated expression of mouse B-myb. Oncogene 7(9):1885–1890, PMID: 1501895

    PubMed  CAS  Google Scholar 

  10. Lam E, Bennett JD, Watson RJ (1995) Cell-cycle regulation of human B-myb transcription. Gene 160:277–281, PMID: 7642110

    Article  PubMed  CAS  Google Scholar 

  11. Sala A (2005) B-MYB, a transcription factor implicated in regulating cell cycle, apoptosis and cancer. Eur J Cancer 41:2479–2484, PMID: 16198555

    Article  PubMed  CAS  Google Scholar 

  12. Nakagoshi H, Takemoto Y, Ishii S (1993) Functional domains of the human B-MYB gene product. J Biol Chem 268:14161–14167, PMID: 8314782

    PubMed  CAS  Google Scholar 

  13. Lam E, Morris JDH, Davies R et al (1994) HPV16 E7 oncoprotein deregulates B-myb expression: correlation with targeting of p107/E2F complexes. EMBO J 13(4):871–878, PMID: 8112300

    PubMed  CAS  PubMed Central  Google Scholar 

  14. Sheffer M, Bacolod MD, Zuk O et al (2009) Association of survival and disease progression with chromosomal instability: a genomic exploration of colorectal cancer. Proc Natl Acad Sci U S A 106(17):7131–7136, PMID: 19359472

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  15. Thorner AR, Hoadley KA, Parker JS et al (2009) In vitro and in vivo analysis of B-Myb in basal-like breast cancer. Oncogene 28(5):742–751, PMID: 19043454

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  16. Astbury K, McEvoy L, Brian H et al (2011) MYBL2 (B-MYB) in cervical cancer: putative biomarker. Int J Gynecol Cancer 21(2):206–212, PMID: 21270603

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This work was carried out within the CERVIVA research programme www.cerviva.ie. CERVIVA is funded by the Health Research Board, Ireland, The Irish Cancer Society, Friends of the Coombe, Enterprise Ireland, The Meath Foundation, The Royal City of Dublin Trust, and the European Union’s 7th framework program.

Author information

Authors and Affiliations

  1. Department of Histopathology, University of Dublin, Trinity College, Dublin, Ireland

    Cara M. Martin, Katharine Astbury & John J. O’Leary

  2. Department of Pathology, The Coombe Women and Infants University Hospital, Dublin 8, Ireland

    Cara M. Martin, Louise Kehoe, Jacqueline Barry O’Crowley & John J. O’Leary

  3. Department of Obstetrics and Gynaecology, Galway University Hospital, Galway, Ireland

    Katharine Astbury

  4. Department of Obstetrics and Gynaecology, University of Dublin, Trinity College, Trinity Centre for Health Sciences, St. James’s Hospital, Dublin 8, Ireland

    Sharon O’Toole

Authors
  1. Cara M. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katharine Astbury
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Louise Kehoe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jacqueline Barry O’Crowley
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sharon O’Toole
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John J. O’Leary
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cara M. Martin .

Editor information

Editors and Affiliations

  1. Touro University California, Vallejo, California, USA

    Daniel Keppler

  2. Touro University California, Vallejo, California, USA

    Athena W. Lin

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Martin, C.M., Astbury, K., Kehoe, L., O’Crowley, J.B., O’Toole, S., O’Leary, J.J. (2015). The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer. In: Keppler, D., Lin, A. (eds) Cervical Cancer. Methods in Molecular Biology, vol 1249. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2013-6_18

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2013-6_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2012-9

  • Online ISBN: 978-1-4939-2013-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation