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Regulation of PTPID mRNA by Peptide Growth Factors in the Human Endometrial Cell Line HEC-I-A

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

To assess, in the human endometrial cell line HEC-1-A, the presence of protein tyrosine phosphatase 1 (PTP1D) and the possible regulation of its mRNA expression by mitogens such as forskolin (an agent that increases intracellular cyclic adenosine monophosphate [cAMP] levels), epidemial growth factor (EGF), and insulin-like growth factor-I (IGF-1).

Methods

Cells were grown to confluence and maintained in serum-free media for 24 hours before treatment. Cells were exposed to forskolin, EGF, and IGF-I for increasing time periods (0, 1, 3, 6, and 24 hours), and PTP1D mRNA expression was determined by Northern blot analysis. In addition, cells were incubated with increasing doses of forskolin (final concentrations: 1, 5, 10, 20, and 30 μmol/L) for 6 hours.

Results

When treated with the various mitogens, cells increased their stimulation of PTP1D mRNA expression in a time- and dose-dependent fashion. Specifically, forskolin, EGF, and IGF-I induced maximal mRNA expression at 6, 3, and 6 hours, respectively. Expression induced by forskolin, EGF, and IGF-I was five, three, and six times control levels, respectively. At a dose of 10 μmol/L, forskolin induced PTP1D mRNA expression almost two times higher than control values.

Conclusion

These data suggest that in human endometrial carcinomas, cAMP, EGF, and IGF-I may regulate the expression of PTP1D mRNA, which may, in turn, play a role in uncontrolled cell proliferation and neoplastic transformation.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Michigan Medical Center, 6428 Med Sci I, 1301 Catherine Street, Ann Arbor, MI, 48109-0617, USA

    James J. Burke II MD, Francisco Talavera PhD & K. M. J. Menon PhD

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  1. James J. Burke II MD
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  2. Francisco Talavera PhD
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  3. K. M. J. Menon PhD
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Burke, J.J., Talavera, F. & Menon, K.M.J. Regulation of PTPID mRNA by Peptide Growth Factors in the Human Endometrial Cell Line HEC-I-A. Reprod. Sci. 4, 310–315 (1997). https://doi.org/10.1177/107155769700400608

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  • DOI: https://doi.org/10.1177/107155769700400608

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