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Chemical Identification of Botanical Components Using Liquid Chromatography/Mass Spectrometry

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Abstract

The chemical identification of botanical products can be accomplished by obtaining a “profile “ of the mixture components. Changes in this profile are powerful indicators of variations due to such factors as process differences, raw material source, or storage stability. Such a profile may be obtained using an analytical protocol based on liquid chromatography/mass spectrometry (LC/MS) techniques. This paper describes an analytical strategy using LC/MS which can be used to rapidly characterize the components in complex materials and to support their research, development, and manufacturing. This strategy utilizes standard method approaches, a rapid “template” approach for structure identification, and the application of databases of compounds to facilitate identification and consistency throughout the product lifetime. This analytical strategy is illustrated with the LC/MS characterization of mixtures of taxanes from Taxus Brevifolia extracts and development of a taxane database. This LC/MS-based strategy provides effective information for the accelerating medicinal field.

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Authors and Affiliations

  1. Bristol-Myers Squibb Pharmaceutical Research Institute, 1 Squibb Drive, New Brunswick, NJ, 08903-0191, USA

    Edward H. Kerns, Kevin J. Volk, Jeffrey L. Whitney, Robyn A. Rourick & Mike S. Lee

  2. Wallingford, Connecticut, USA

    Edward H. Kerns, Kevin J. Volk, Jeffrey L. Whitney, Robyn A. Rourick & Mike S. Lee

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  1. Edward H. Kerns
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  2. Kevin J. Volk
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  4. Robyn A. Rourick
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  5. Mike S. Lee
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Kerns, E.H., Volk, K.J., Whitney, J.L. et al. Chemical Identification of Botanical Components Using Liquid Chromatography/Mass Spectrometry. Ther Innov Regul Sci 32, 471–485 (1998). https://doi.org/10.1177/009286159803200220

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