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Salt-modulated structure of polyelectrolyte-macroion complex fibers

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Abstract

The structure and stability of strongly charged complex fibers, formed by complexation of a single long semi-flexible polyelectrolyte chain and many oppositely charged spherical macroions, are investigated numerically at the ground-state level using a chain-sphere cell model. The model takes into account chain elasticity as well as electrostatic interactions between charged spheres and chain segments. Using a numerical optimization method based on a periodically repeated unit cell, we obtain fiber configurations that minimize the total energy. The optimal fiber configurations exhibit a variety of helical structures for the arrangement of macroions including zig-zag, solenoidal and beads-on-a-string patterns. These structures result from the competition between attraction between spheres and the polyelectrolyte chain (which favors chain wrapping around the spheres), chain bending rigidity and electrostatic repulsion between chain segments (which favor unwrapping of the chain), and the interactions between neighboring sphere-chain complexes which can be attractive or repulsive depending on the system parameters such as salt concentration, macroion charge and chain length per macroion (linker size). At about physiological salt concentration, dense zig-zag patterns are found to be energetically most stable when parameters appropriate for the DNA-histone system in the chromatin fiber are adopted. In fact, the predicted fiber diameter in this regime is found to be around 30 nanometers, which roughly agrees with the thickness observed in in vitro experiments on chromatin. We also find a macroion (histone) density of 5–6 per 11nm which agrees with results from the zig-zag or cross-linker models of chromatin. Since our study deals primarily with a generic chain-sphere model, these findings suggest that structures similar to those found for chromatin should also be observable for polyelectrolyte-macroion complexes formed in solutions of DNA and synthetic nano-colloids of opposite charge. In the ensemble where the mean linear density of spheres on the chain is fixed, the present model predicts a phase separation at intermediate salt concentrations into a densely packed complex phase and a dilute phase.

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Authors and Affiliations

  1. Department of Physics, Technical University of Munich, 85748, Garching, Germany

    Hoda Boroudjerdi, Ali Naji & Roland R. Netz

  2. Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, CB3 0WA, Cambridge, UK

    Ali Naji

  3. School of Physics, Institute for Research in Fundamental Sciences (IPM), 19395-5531, Tehran, Iran

    Ali Naji

  4. Fachbereich Physik, Freie Universität Berlin, Arnimalle 14, 14195, Berlin, Germany

    Roland R. Netz

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  1. Hoda Boroudjerdi
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  2. Ali Naji
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Correspondence to Ali Naji.

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Boroudjerdi, H., Naji, A. & Netz, R.R. Salt-modulated structure of polyelectrolyte-macroion complex fibers. Eur. Phys. J. E 34, 72 (2011). https://doi.org/10.1140/epje/i2011-11072-1

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