Abstract:
We observed fluctuations of elongated DNA molecules by fluorescence microscopy. The molecules are fixed at both ends and undulate. Mode analysis of the thermally excited undulations of the labeled DNA molecules gives access to the spectral density of the amplitude fluctuations. From these measurements we estimate the tension acting on the DNA molecules. We found the forces to be within the entropic elasticity range of a typical DNA molecule (measured on the single-molecule level).
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Received: 11 November 2002 / Accepted: 12 May 2002 / Published online: 4 June 2003
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ID="a"Present address: Center for Studies in Physics and Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. e-mail: zoherg@rockefeller.edu
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Gueroui, Z., Freyssingeas, E., Place, C. et al. Transverse fluctuation analysis of single extended DNA molecules. Eur Phys J E 11, 105–108 (2003). https://doi.org/10.1140/epje/i2002-10150-9
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DOI: https://doi.org/10.1140/epje/i2002-10150-9