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Experimental opisthorchiasis: Study of blood cell composition, hematopoiesis, and startle reflex in laboratory animals

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Russian Journal of Genetics: Applied Research

Abstract

The Opisthorchis felineus (O. felineus), one of the species from the Opisthorchiidae family, causes severe disorders in the human and animal organism; therefore, it is the subject of topical scientific studies. In this work, a comparative study of the influence of the O. felineus invasions on the change in the blood cell composition, bone marrow hematopoiesis, and behavioral startle reflex was conducted in the C57BL/6 mice and Syrian hamsters two weeks after infection. Significant interspecies differences in many indices were found. It was established that the relative weight of the spleen (the main peripheral immune system organ) is significantly larger in mice than in hamsters. Moreover, the O. felineus invasion caused the hypertrophy of the spleen only in mice. More pronounced deviations from the norm in the blood cell composition, that were accompanied by the activation of myelo- and erythropoiesis, were observed in hamsters. The established changes in the blood in mice were not accompanied by changes in the bone marrow colony-forming activity. Mice also differed from hamsters by the startle reaction to the acoustic signal both by the severity of the reaction and prepulse inhibition value. No habituation reaction to the sound signal was observed in the infected hamsters. In addition, maturation of the O. felineus worms in hamsters occurred earlier than in mice. The data obtained indicate the increased resistance of mice to the O. feluneus infection; however, they do not exclude the possibility of using mice as a model during the study of the processes occurring in the host organism against the background of the experimental opisthorchiasis development.

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Authors and Affiliations

  1. Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    D. F. Avgustinovich, G. B. Vishnivetskaya, A. V. Katokhin, M. N. Lvova, E. V. Kashina, N. P. Bondar, N. A. Feofanova & V. A. Mordvinov

  2. Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russia

    I. A. Orlovskaya, L. B. Toporkova & N. A. Feofanova

  3. Research Institute of Molecular Biology and Biophysics, Novosibirsk, Russia

    V. A. Mordvinov

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  1. D. F. Avgustinovich
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  2. I. A. Orlovskaya
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  3. L. B. Toporkova
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  4. G. B. Vishnivetskaya
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  5. A. V. Katokhin
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  6. M. N. Lvova
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  7. E. V. Kashina
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  8. N. P. Bondar
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  9. N. A. Feofanova
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  10. V. A. Mordvinov
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Correspondence to D. F. Avgustinovich.

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Original Russian Text © D.F. Avgustinovich, I.A. Orlovskaya, L.B. Toporkova, G.B. Vishnivetskaya, A.V. Katokhin, M.N. Lvova, E.V. Kashina, N.P. Bondar, N.A. Feofanova, V.A. Mordvinov, 2016, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2016, Vol. 20, No. 2, pp. 155–164.

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Avgustinovich, D.F., Orlovskaya, I.A., Toporkova, L.B. et al. Experimental opisthorchiasis: Study of blood cell composition, hematopoiesis, and startle reflex in laboratory animals. Russ J Genet Appl Res 7, 82–92 (2017). https://doi.org/10.1134/S2079059717010038

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