Abstract
Nuclear receptors LXRα/β and PPARγ play an important role in the regulation of lipid metabolism and reverse cholesterol transport. However, the effect of the expression of LXRα/β and PPARγ genes in macrophages on the development of atherosclerosis remains poorly studied. We determined the levels of LXRα mRNA, LXRβ mRNA, and PPARγ mRNA by a real-time polymerase chain reaction in macrophages cultivated for 5 days with macrophage colony-stimulating factor (M-CSF). The levels of LXRβ mRNA and PPARγ mRNA were lower in patients with coronary artery stenosis than in the control group, p < 0.001. The groups did not differ in the content of LXRα mRNA (p = 0.17). We suggest that the level of expression of LXRβ and PPARγ genes in macrophages may be a significant factor associated with the development of atherosclerosis.
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Amoruso, A., Bardelli, C., Fresu, L.G., et al., Peroxisome proliferator-activated receptor-expression in monocyte/macrophages from coronary artery disease patients and possible gender differences, J. Pharmacol. Exp. Ther., 2009, vol. 331, no. 2, pp. 531–538.
Apostoli, A.J. and Nicol, C.J., PPAR medicines and human disease: the ABCs of it all, PPAR Res., 2012, p. 504918.
Bensinger, S.J. and Tontonoz, P., Integration of metabolism and inflammation by lipid-activated nuclear receptors, Nature, 2008, vol. 454, pp. 470–477.
Calkin, A.C. and Tontonoz, P., Transcriptional integration of metabolism by the nuclear sterol-activated receptors LXR and FXR, Nat. Rev. Mol. Cell Biol., 2012, vol. 13, no. 4, pp. 213–24.
Giaginis, C., Klonaris, C., Katsargyris, A., et al., Correlation of Peroxisome proliferator-activated receptorgamma (PPAR-gamma) and retinoid X receptor-alpha (RXR-alpha) expression with clinical risk factors in patients with advanced carotid atherosclerosis, Med. Sci. Monit., 2011, vol. 17, no. 7, pp. 381–391.
Li, A.C., Binder, C.J., Gutierrez, A., et al., Differential inhibition of macrophage foam-cell formation and atherosclerosis in mice by PPARalpha, beta/delta, and gamma, J. Clin. Invest., 2004, vol. 114, no. 11, pp. 1564–1576.
Meurs, I., Van Eck, M., and Van Berkel, T.J., High-density lipoprotein: key molecule in cholesterol efflux and the prevention of atherosclerosis, Curr. Pharm. Des., 2010, vol. 16, pp. 1445–1467.
Oosterveer, M.H., Grefhorst, A., Groen, A.K., et al., The liver X receptor: control of cellular lipid homeostasis and beyond: implications for drug design, Prog. Lipid Res., 2010, vol. 49, pp. 343–352.
Rader, D.J. and Pure, E., Lipoproteins, macrophage function, and atherosclerosis: beyond the foam cell?, Cell Metab., 2005, vol. 1, pp. 223–230.
Soumian, S., Gibbs, R., Davies, A., et al., mRNA expression of genes involved in lipid efflux and matrix degradation in occlusive and ectatic atherosclerotic disease, J. Clin. Pathol., 2005, vol. 58, pp. 1255–1260.
Sueyoshi, S., Mitsumata, M., Kusumi, Y., et al., Increased expression of peroxisome proliferator-activated receptor (PPAR)-alpha and PPAR-gamma in human atherosclerosis, Pathol. Res. Pract., 2010, vol. 206, no. 7, pp. 429–438.
Wahli, W. and Michalik, L., PPARs at the crossroads of lipid signaling and inflammation, Trends Endocrinol. Metab., 2012, vol. 23, no. 7, pp. 351–363.
Zar, J.H., Biostatistical Analys, 4th ed., Prentice Hall, 1998.
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Original Russian Text © Ye.P. Demina, V.V. Miroshnikova, N.V. Mayorov, V.V. Davydenko, A.L. Schvartzman, 2014, published in Ekologicheskaya Genetika, 2014, Vol. 12, No. 1, pp. 14–18.
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Demina, Y.P., Miroshnikova, V.V., Mayorov, N.V. et al. Reduction of the level of LXRβ mRNA and PPARγ mRNA in macrophages stimulated with a macrophage colony-stimulating factor in patients with atherosclerosis. Russ J Genet Appl Res 5, 155–158 (2015). https://doi.org/10.1134/S2079059715020021
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DOI: https://doi.org/10.1134/S2079059715020021