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Comparative molecular genetic study of the Asian population of Drosophila mercatorum (Diptera; Drosophilidae) at the COI gene fragment and the ITS1–ITS2 region of the rRNA genes

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Russian Journal of Genetics: Applied Research

Abstract

Drosophila mercatorum is a species of neotropical origin. It is represented by two subspecies, marcatorum and pararepleta. Synanthropic subspecies D. mercatorum mercatorum spread into Eurasia in the mid-20th century, and to date, it is widespread over the territory of Europe and the former Soviet Union. The experiments were performed using the specimens of D. m. mercatorum from natural Asian populations, laboratory stock 2328 of D. mercatorum (displaying some morphological differences from Asian wild-type flies), the specimens of D. busckii and D. virilis, and the COI gene sequence of D. mercatorum from NCBI database, accession number, DQ471607. The specimens were investigated for the variability of the standard DNA sequences used for species identification (the cytochrome oxidase subunit I (COI) gene fragment and the ITS1–ITS2 region of rRNA genes). Sequences of the COI gene fragment from Asian D. m. mercatorum were identical between the specimens, but differed from the sequences of stock 2328, as well as from the NCBI sequence. Asian populations of D. mercatorum and stock 2328 were identical in the ITS1–ITS2 sequences. Low sequence divergence between the COI gene fragments, along with the absence of differences at the ITS1–ITS2 region of the rRNA genes indicate that the difference between the Asian populations of D. m. mercatorum and morphologically different stock 2328 are within the frames of intraspecies divergence.

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References

  • Altukhov, Yu.P., Geneticheskie protsessy v populyatsiyakh (TRANSLATION), Moscow: IKTs “Akademkniga”, 2003.

    Google Scholar 

  • Altukhov, Yu.P. and Salmenkova, E.A., DNA polymorphism in population genetics, Russ. J. Genet., 2002, vol. 38, no. 9, pp. 989–1008.

    Article  CAS  Google Scholar 

  • Bächli, G. and Rocha, PiteM.T., Drosophilidae of the Palearctic region, in The Genetics and Biology of Drosophila, Ashburner, M., Carson, H.L., and Thompson, N., Jr., Eds., Acad. Press, 1981, vol. 3a, pp. 169–196.

    Google Scholar 

  • Bandelt, H.-J., Forster, P., and Rohl, A., Median-joining networks for inferring intraspecific phylogenies, Mol. Biol. Evol., 1999, vol. 16, no. 1, pp. 37–48.

    Article  CAS  PubMed  Google Scholar 

  • Beebe, N.W., Maung, J., Hurk, A.F., Ellis, J.T., and Cooper, R.D., Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and New Guinea, Insect Mol. Biol., 2001, vol. 10, no. 5, pp. 407–414.

    Article  CAS  PubMed  Google Scholar 

  • Bender, W., Spierer, P., Hognes, D.S., and Chambon, P., Chromosomal walking and jumping to isolate DNA from Ace and rosy loci of bithorax loci in Drosophila melanogaster, J. Mol. Biol., 1983, vol. 168, no. 1, pp. 17–33.

    Article  CAS  PubMed  Google Scholar 

  • Bennett, H.J., Jenkinsonm, L.S., and Davis, A.J., Drosophila mercatorum Patterson and Wheeler 1942 (Diptera: Drosophilidae): first record for the British isles, Dros. Inform. Serv., 1995, vol. 76, pp. 94–95.

    Google Scholar 

  • David, J.R. and Tsacas, L., Cosmopolitan, subcosmopolitan, and widespread species: different strategies with in the Drosophilid family (Diptera), S. R. Soc. Biogeogr., 1980, vol. 57, no. 1, pp. 11–26.

    Google Scholar 

  • Dobzhansky, Th. and Pavan, C., Studies on Brasilian species of Drosophila, Separata do Bol. Facul. Fil. Cien. e Letr. Univ. S. Paolo, no. 36, Biologia Geral., 1943, no. 4.

    Google Scholar 

  • Dover, G. and Flavell, R., Molecular coevolution: DNA divergence and the maintenance of function, Cell, 1984, vol. 38, no. 3, pp. 622–623.

    Article  CAS  PubMed  Google Scholar 

  • Hebert, P.D.N., Cywinska, A., and Ball, S., Biological identifications through DNA barcodes, Proc. R. Soc. London, Ser. B, 2003, vol. 270, pp. 313–321.

    Article  CAS  Google Scholar 

  • Hurst, G.D.D. and Jiggins, F.M., Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts, Proc. Roy. Soc. B: Biol. Sci., 2005, vol. 272, pp. 1525–1534.

    Article  CAS  Google Scholar 

  • Ilinsky, Yu.Yu. and Zakharov, I.K., Infection of the Uman’ population of Drosophila melanogaster with the cytoplasmic endosymbiont Wolbachia, Dokl. Biol. Sci., 2007, vol. 413, pp. 166–168.

    Article  Google Scholar 

  • Ivannikov, A.V. and Zakharov, I.K., Distribution of Drosophila mercatorum into Eurasian continent, Dros. Inform. Serv., 1995, no. 76, p. 107.

    Google Scholar 

  • Ivannikov, A.V., Zakharov, I.K., and Skibitsky, E.E., Synantropic Drosophila species in the Ukraine and Moldova, Dros. Inform. Serv., 1993, no. 72, p. 87.

    Google Scholar 

  • Ivannikov, A.V., Katokhin, A.V., and Sidorenko, V.S., List of Drosophilidae (Diptera) of West Siberia, Far Eastern Entomol., 1998, no. 57, pp. 12–16.

    Google Scholar 

  • Kimura, M., A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences, J. Mol. Evol., 1980, vol. 16, pp. 111–120.

    Article  CAS  PubMed  Google Scholar 

  • Kraaijeveld, A.R., First record of Drosophila mercatorum (Diptera: Drosophilidae) in northwestern Europe, Entomologische Berichten, Amsterdam, 1992, vol. 52, p. 32 (cited by: Bennett, H.J., Jenkinson, L.S., and Davis, A.J., 1995).

    Google Scholar 

  • Michel, A.P., Guelbeogo, W.M., Grushko, O.B., et al., Molecular differentiation between chromosomally defined incipient species of Anopheles funestus, Insect Mol. Biol., 2005, vol. 14, no. 4, pp. 375–387.

    Article  CAS  PubMed  Google Scholar 

  • Monclu-s, M., Distribución y ecología de Drosophilidos en España. I. Especies de Drosophila de la region Catalana, Genet. Iber., 1964, vol. 16, pp. 143–165.

    Google Scholar 

  • Monclu-s, M., Distributión y ecología de Drosophilidos en España. II Especies de Drosophila de las Islas Canarias, con la description de una nueva especie, Biol. R. Soc. Espanola Hist. Nat. (Biol.), 1976, vol. 74, pp. 197–213.

    Google Scholar 

  • Monclu-s, M., Drosophilidae of Madeira, with the description of Drosophila madeirensis n. sp., Soderdruckaus Z. f. zool. Systematik u. Evolutionstorschung, 1984, vol. 22, no. 2, pp. 94–103.

    Article  Google Scholar 

  • Novikov, Yu.M., Shevchenko, A.I., and Vaulin, O.V., The molecular-genetic divergence of cryptic species of the taxon Anopheles messeae (Diptera: Culicidae) and the phylogeny of the complex maculipennis, Vestn. Tomsk. Gos. Univ. (Suppl.), Tomsk: Tomsk. Gos. Univ., 2004, vol. 10, pp. 69–77.

    Google Scholar 

  • Patterson, J.T. and Wheeler, M.R., Description of new species of the subgenera Hirtodrosophila and Drosophila, Univ. Texas Publ., 1942, vol. 4213, pp. 67–109.

    Google Scholar 

  • Prevosti, A., Two newly introduced species of drosophila found in Europe, Dros. Inform. Serv., 1953, vol. 27, p. 110.

    Google Scholar 

  • Sidorenko, V.S., Family Drosophilidae, in Opredelitel’ nasekomykh Dal’nego Vostoka Rossii (Insects of Russian Far East: An Identification Guide), Vladivostok: Dal’nauka, 2001, vol. 4, part 2, pp. 211–241.

    Google Scholar 

  • Tamura, K., Peterson, D., Peterson, N., et al., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, no. 10, pp. 2731–2739.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Vaulin, O.V. and Novikov, Yu.M., Geographic variation of ITS 2 rDNA and COI mtDNA in cryptic species of the malaria mosquito Anopheles messeae Fall. (Diptera: Culicidae), Inform. Vestn. VOGiS, 2010, vol. 14, no. 3, pp. 546–557.

    Google Scholar 

  • Vaulin, O.V. and Novikov, Yu.M., YM Polymorphism and interspecific variability of the nucleotide sequence of the cytochrome oxidase subunit I (COI) gene from the sibling species A and B Anopheles messeae and An. beklemishevi (Diptera: Culicidae), Vavilov. Zh. Genet. Selekts., 2012, vol. 16, no. 2, pp. 358–368.

    Google Scholar 

  • Wasserman, M., Evolution of the repleta group, in The Genetics and Biology of Drosophila, Ashburner, M., Carson, H.L., and Thompson, J.N., Jr., Eds., Acad. Press, 1981, vol. 3b, pp. 61–139.

    Google Scholar 

  • Wheeler, M.R., The Drosophilidae: a taxonomic overview, in The Genetics and Biology of Drosophila, Ashburner, M., Carson, H.L., and Thompson, J.N., Jr., Eds., Acad. Press, 1981, vol. 3a, pp. 1–97.

    Google Scholar 

  • Wilkerson, R.C., Reinert, J.F., and Li, C., Ribosomal DNA ITS2 sequences differentiation six species in the Anopheles crucians complex (Diptera: Culicidae), J. Med. Entomol., 2004, vol. 41, no. 3, pp. 392–401.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    A. V. Ivannikov, O. V. Vaulin, Yu. A. Koromyslov & I. K. Zakharov

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  1. A. V. Ivannikov
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  2. O. V. Vaulin
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  3. Yu. A. Koromyslov
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  4. I. K. Zakharov
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Correspondence to A. V. Ivannikov.

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Original Russian Text © A.V. Ivannikov, O.V. Vaulin, Yu.A. Koromyslov, I.K. Zakharov, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 2, pp. 308–314.

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Ivannikov, A.V., Vaulin, O.V., Koromyslov, Y.A. et al. Comparative molecular genetic study of the Asian population of Drosophila mercatorum (Diptera; Drosophilidae) at the COI gene fragment and the ITS1–ITS2 region of the rRNA genes. Russ J Genet Appl Res 4, 533–538 (2014). https://doi.org/10.1134/S2079059714060100

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  • DOI: https://doi.org/10.1134/S2079059714060100

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