Abstract
Using pea single mutant lines SGEFix−-2 (sym33) and RisFixV (sym42), which are characterized by different abnormalities during symbiotic nodule development, including thickening of infection threads’ walls, a double mutant RBT4 line, carrying a pair of symbiotic genes sym33 and sym42 was constructed. The epistasis of the mutant allele sym33 over the mutant allele sym42 with respect to the histological and ultra-structural organization of nodules was shown. Thus, it was demonstrated that the Sym33 gene functions earlier in symbiotic nodule development than the Sym42 gene.
Similar content being viewed by others
References
Borisov, A.Y., Morzhina, E.V., Kulikova, O.A., et al., New symbiotic mutants of pea (Pisum sativum L.) affecting either nodule initiation or symbiosome development, Symbiosis, 1992, vol. 14, pp. 297–313.
Borisov, A.Y., Rozov, S.M., Tsyganov, V.E., et al., Sequential functioning of sym13 and sym31, two genes affecting symbiosome development in root nodules of pea (Pisum sativum L.), Mol. Gen. Genet., 1997, vol. 254, pp. 592–598.
Brewin, N.J., Plant cell wall remodelling in the Rhizobium-legume symbiosis, Critic. Rev. Plant Sci., 2004, vol. 23, pp. 293–316.
Engvild, K.J., Nodulation and nitrogen fixation mutants of pea (Pisum sativum), Theor. Appl. Genet., 1987, vol. 74, pp. 711–713.
Kosterin, O.E. and Rozov, S.M., Mapping of the new mutation blb and the problem of integrity of linkage group I, Pisum Genet., 1993, vol. 25, pp. 27–31.
Lévy, J., Bres, C., Geurts, R., et al., A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses, Science, 2004, vol. 303, pp. 1361–1364.
Morzhina, E.V., Tsyganov, V.E., Borisov, A.Y., et al., Four developmental stages identified by genetic dissection of pea (Pisum sativum L.) root nodule morphogenesis, Plant Sci., 2000, vol. 155, pp. 75–83.
Novák, K., Pesina, K., Nebesarova, J., et al., Symbiotic tissue degradation pattern in the ineffective nodules of three nodulation mutants of pea (Pisum sativum L.), Ann. Bot., 1995, vol. 76, pp. 303–313.
Oldroyd, G.E. and Downie, J.A., Coordinating nodule morphogenesis with rhizobial infection in legumes, Annu. Rev. Plant. Biol., 2008, vol. 59, pp. 519–546.
Ovchinnikova, E., Journet, E.P., Chabaud, M., et al., IPD3 controls the formation of nitrogen-fixing symbiosomes in pea and Medicago spp., Mol. Plant-Microbe Interact., 2011, vol. 24, pp. 1333–1344.
Rae, A.L., Bonfante-Fasolo, P., and Brewin, N.J., Structure and growth of infection threads in the legume symbiosis with Rhizobium leguminosarum, Plant J., 1992, vol. 2, pp. 385–395.
Rathbun, E.A., Naldrett, M.J., and Brewin, N.J., Identification of a family of extensin-like glycoproteins in the lumen of Rhizobium-induced infection threads in pea root nodules, Mol. Plant-Microbe Interact., 2002, vol. 15, pp. 350–359.
Safronova, V.I. and Novikova, N.I., Comparison of two methods for root nodule bacteria preservation: lyophilization and liquid nitrogen freezing, J. Microbiol. Methods, 1996, vol. 24, pp. 231–237.
Tsyganov, V.E., Morzhina, E.V., Stefanov, S.Y., et al., The pea (Pisum sativum L.) genes sym33 and sym40 control infection thread formation and root nodule functioning, Mol. Gen. Genet., 1998, vol. 259, pp. 491–503.
Tsyganov, V.E., Voroshilova, V.A., Herrera-Cervera, J.A., et al., Developmental downregulation of rhizobial genes as a function of symbiosome differentiation in symbiotic root nodules of Pisum sativum L., New Phytol., 2003, vol. 159, pp. 521–530.
Tsyganov, V.E., Seliverstova, E.V., Voroshilova, V.A., et al., Double mutant analysis of sequential functioning of pea genes (Pisum sativum L.) Sym13, Sym33, and Sym40 during the symbiotic nodule development, Ekol. Genet., 2010, vol. 8, no. 2, pp. 3–8.
Tsyganov, V.E., Voroshilova, V.A., Rozov, S.M., et al., A new series of pea symbiotic mutants induced in the line SGE, Ekol. Genet., 2012, vol. 10, no. 1, pp. 19–26.
Tsyganova, A.V., Tsyganov, V.E., Findlay, K.K., et al., Distribution of legume arabinogalactan protein-extensin (AGPE) glycoproteins in symbiotically defective pea mutants with abnormal infection threads, Tsitologiia, 2009, vol. 51, no. 1, pp. 53–62.
Tsyganova, A.V. and Tsyganov, V.E., The role of rhizobial surface components in symbiotic interactions with legumes, Usp. Sovrem. Biol., 2012, vol. 132, no. 2, pp. 211–222.
Tsyganova, A.V., Kitaeva, A.B., Brewin, N.J., and Tsyganov, V.E., Cellular mechanisms of nodule development in legume plants, S.-Khoz. Biol., 2011, no. 3, pp. 34–40.
Voroshilova, V.A., Boesten, B., Tsyganov, V.E., et al., Effect of mutations in Pisum sativum L. genes (sym13, sym31, sym33, sym40) blocking different stages of nodule development on the expression of late symbiotic genes in Rhizobium leguminosarum bv. viciae, Mol. Plant-Microbe Interact., 2001, vol. 14, pp. 471–476.
Wang, T.L., Wood, E.A., and Brewin, N.J., Growth regulators, Rhizobium, and nodulation of peas, Planta, 1982, vol. 155, pp. 345–349.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.E. Tsyganov, A.V. Tsyganova, V.A. Voroshilova, A.Y. Borisov, I.A. Tikhonovich, 2012, published in Ekologicheskaya Genetika, 2012, Vol. 10, No. 4, pp. 50–55.
Rights and permissions
About this article
Cite this article
Tsyganov, V.E., Tsyganova, A.V., Voroshilova, V.A. et al. Analysis of the interaction of pea (Pisum sativum L.) symbiotic genes Sym33 and Sym42 whose mutations result in abnormalities during infection thread development. Russ J Genet Appl Res 4, 83–87 (2014). https://doi.org/10.1134/S2079059714020105
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079059714020105