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Researching the mechanisms of PHO3 gene regulation depending on the nitrogen source in medium in yeast Saccharomyces cerevisiae

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Russian Journal of Genetics: Applied Research

Abstract

Delicate regulation of gene expression is performed through competition of transcriptional factors for promoters that provides punctual cell response to environmental changes. Gene PHO3 of yeast S. cerevisiae encodes the constitutive acid phosphatase. In this work we have researched the genetic control of PHO3 gene expression depending on the source of nitrogen in medium. The PHO3 expression level was proven to decrease with yeast using a poor nitrogen source like urea. Possible regulatory mechanisms for gene PHO3 were revealed involving a repressor of nitrogen regulated genes Gzf3p and ubiquitin ligase Rsp5p.

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Authors and Affiliations

  1. Faculty of Biology and Soil Science, Department of Genetics and Breeding, St. Petersburg State University, Universitetskaya nab. 7-9, St. Petersburg, Russia

    V. A. Savinov, A. Yu. Fizikova, A. M. Rumyantsev & E. V. Sambuk

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  1. V. A. Savinov
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  2. A. Yu. Fizikova
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  3. A. M. Rumyantsev
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  4. E. V. Sambuk
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Correspondence to V. A. Savinov.

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Original Russian Text © V.A. Savinov, A.Yu. Fizikova, A.M. Rumyantsev, E.V. Sambuk, 2011, published in Ekologicheskaya Genetika, 2011, Vol. 9, No. 4, pp. 70–78.

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Savinov, V.A., Fizikova, A.Y., Rumyantsev, A.M. et al. Researching the mechanisms of PHO3 gene regulation depending on the nitrogen source in medium in yeast Saccharomyces cerevisiae . Russ J Genet Appl Res 2, 405–412 (2012). https://doi.org/10.1134/S207905971205005X

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  • DOI: https://doi.org/10.1134/S207905971205005X

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