Skip to main content
SpringerLink
Log in

Symbiogenetics and breeding of a macrosymbiont for increased nitrogen fixation capacity with special reference to the pea (Pisum sativum L.)

  • Published:
Russian Journal of Genetics: Applied Research

Abstract

This article presents the results of long-term experimental studies on symbiogenetics and breeding of macrosymbionts in a system of legume-rhizobial symbiosis with the pea (Pisum sativum L.) as an example. The collection of pea symbiotic mutants was created and genetically studied. The new symbiotic genes were identified and localized on a Pisum chromosome map. The dominant symbiotic mutants characterized by high productivity and active nitrogen fixation were induced in the pea for the first time. Certain physiological peculiarities of the auxin and gibberellin phytohormone content were studied in symbiotic mutants. The differences in the activity of nitrogen fixation during ontogeny were revealed for numerous mutants. The forms with a prolonged period of active nitrogen fixation were isolated. A new approach to estimate the efficiency of legume-rhizobial symbiosis was proposed. The method of use of symbiotic mutants in breeding for higher efficiency of nitrogen fixation was proposed for the first time. This method is based on the interaction between different sym genes within one genotype. A series of productive constant recurrent lines of the seventh generation was designed which exceed commercial pea cultivars in nodulation and nitrogen fixation activity. Lines are characterized by accumulation of higher root biomass with increased nitrogen content in them. This approach allows their use in agricultural production instead of expensive organic and mineral fertilizers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Vavilov, P.P. and Posypanov, G.S., Bobovye kul’tury i problema rastitel’nogo belka (Legume Cultures and Problem of Plant Protein), Moscow, 1983.

  • Gyuli-Zade, V.Z., Immunoanaliz i Izuchenie Rostoreguliruyushchei Funktsii Auksinov (Immune Analysis and Study of Auxine Role in Growth Regulation), Extended Abstract of Cand. Sci. (Biol.) Dissertation, Kazan: Kazanskii Institut Biologii, USSR Academy of Sciences, 1990.

    Google Scholar 

  • Dorosinskii, L.M., Kluben’kovye Bakterii i Nitragin (Root Nodule Bacteria and Nitragin), Leningrad: Kolos, 1970.

    Google Scholar 

  • Zakharov, I.A., 100 Year of Symbiosis Theory, Inform. Vestnik VOGiS, 2009, vol. 13, no. 2, pp. 355–361.

    Google Scholar 

  • Ignatov, V.V., Biological Nitrogen Fixation and Nitrogen Fixing Organisms, Sorosovskii Obrazovat. Zh., 1998, no. 9, pp. 28–33.

  • Kudoyarova, G.R., Veselov, S.Yu., Erkeev, M.I., and Gyuli-Zade, V.Z., Immune Estimation of IAA Content in Maize Seeds with Usage of Labeled Antibodies, Fiziol. Rastenii, 1986, vol. 33, no. 6, pp. 376–377.

    Google Scholar 

  • Margelis, L., Symbiosis in Cell Evolution: Life and Its Environment on the Early Earth, San Francisco, 1981.

  • Nazaryuk, V.M., Sidorova, K.K., Shumny, V.K., and Klenova, M.I., New Method of Estimation of Efficiency of Legume-Rhizobial Symbiosis in Field Conditions, Agrokhimiya, 2003, no. 1, pp. 77–83.

  • Nazaryuk, V.M., Sidorova, K.K., Shumnyi, V.K., and Klenova, M.I., Role of Macrosymbiont’s Genotype in Nitrogen Fixation from Soil and Air, Dokl. Akad. Nauk, 2004, vol. 394, no. 1, pp. 139–141 [Dokl. Biol. Sci. (Engl. Transl.), vol. 394, no. 1, pp. 44–46].

    Google Scholar 

  • Pavlova, Z.B., Dobrodumova, V.V., Kravchenko, L.V., and Lutova, L.A., Characterization of Some Symbiotic Pea Mutants in Traits Connected with Hormone Status, Genetika, 2000, vol. 36, no. 6, pp. 799–804 [Rus. J. Genet. (Engl. Transl.), 2000, vol. 36, no. 6, pp. 656–660].

    PubMed  CAS  Google Scholar 

  • Pavlova, Z.B. and Lutova, L.A., Nodule Formation as Model for Studies on Differentiation in Higher Plants, Genetika, 2000, vol. 36, no. 9, pp. 1173–1188 [Rus. J. Genet. (Engl. Transl.), 2000, vol. 36, no. 9, pp. 975–988].

    PubMed  CAS  Google Scholar 

  • Posypanov, G.S., Metody Izucheniya Biologicheskoi Fiksatsii Azota Vozdukha (Methods of Studies on Biological Fixation of Air Nitrogen), Moscow, 1991.

  • Sidorova, K.K., Nazaryuk, V.M., and Klenova, M.I., RF Patent 2195104, 2002.

  • Sidorova, K.K., Nazaryuk, V.M., Shumnyi, V.K., and Klenova, M.I., New Model for Estimation of Efficiency of Legume-Rhizobial Symbiosis, Dokl. Akad. Nauk, 2001, vol. 380, no. 2, pp. 283–285 [Dokl. (Engl. Transl.), 2001, vol. 380, no. 2, pp. 464–466].

    CAS  Google Scholar 

  • Sidorova, K.K., Stolyarova, S.N., and Katysheva, V.B., Nitrogen Fixing Activity of Pea Mutants, Genetika, 1987, vol. 23, no. 7, pp. 1218–1221.

    Google Scholar 

  • Sidorova, K.K. and Uzhintseva, L.P., Usage of Mutants to Reveal Genes Controlling Symbiotic Features in Pea), Genetika, 1992, vol. 28, no. 4, pp. 144–151.

    Google Scholar 

  • Sidorova, K.K. and Uzhintseva, L.P., Localization of Mutant Gene nod4 Controlling Supernodulation in Pea, Dokl. Akad. Nauk, 1994, vol. 336, no. 6, pp. 847–849.

    Google Scholar 

  • Sidorova, K.K. and Shumnyi, V.K., New Gene nod5-nod5 Controlling Nodulation in Pea (Pisum sativum L.), Dokl. Akad. Nauk, 1997, vol. 353, no. 5, pp. 703–704.

    CAS  Google Scholar 

  • Sidorova, K.K. and Shumnyi, V.K., Studies on Supernodulating Pea (Pisum sativum L.) Mutants, Genetika, 1998, vol. 34, no. 10, pp. 1452–1454 [Rus. J. Genet. (Engl. Transl.), vol. 34, no. 10, pp. 1233–1235].

    Google Scholar 

  • Sidorova, K.K. and Shumnyi, V.K., Genetics of Symbiotic Nitrogen Fixation and Bases of Breeding for Self-Pollinating Cultures (with reference to Pisum sativum L.), Genetika, 1999, vol. 35, no. 11, pp. 1550–1557 [Rus. J. Genet. (Engl. Transl.), 1999, vol. 35, no. 11, pp. 1336–1342].

    Google Scholar 

  • Sidorova, K.K. and Shumnyi, V.K., Construction and Genetic Investigation of Symbiotic Mutants of Pea (Pisum sativum L.), Genetika, 2003, vol. 39, no. 4, pp. 501–509 [Rus. J. Genet. (Engl. Transl.), 2003, vol. 39, no. 4, pp. 406–414].

    PubMed  CAS  Google Scholar 

  • Sidorova, K.K., Shumnyi, V.K., Vlasova, E.Yu., et al., Investigation of Morphological and Symbiotic Features in Ontogeny Dynamics in Supernodulating and Hypernodulating Pea Mutants, Genetika, 2006a, vol. 42, no. 2, pp. 219–225 [Rus. J. Genet. (Eng. Transl.), 2006, vol. 42, no. 2, pp. 158–164].

    PubMed  CAS  Google Scholar 

  • Sidorova, K.K., Shumnyi, V.K., Vlasova, E.Yu., et al., Interaction between Symbiotic Genes nod3 and Nod5 in One Genotype of Pea Pisum sativum L., Dokl. Akad. Nauk, 2008, vol. 419, no. 4, pp. 569–571 [Dokl. (Engl. Transl.), 2008, vol. 419, no. 4, pp. 126–128].

    Google Scholar 

  • Sidorova, K.K., Shumnyi, V.K., Glyanenko, M.N., et al., Investigation of Dominant Symbiotic Mutants of Pea Pisum sativum L., Genetika, 2009, vol. 45, no. 7, pp. 907–912 [Rus. J. Genet. (Eng. Transl.), 2009, vol. 45, no. 7, pp. 794–798].

    PubMed  CAS  Google Scholar 

  • Sidorova, K.K., Shumnyi, V.K., and Mishchenko, T.M., Chromosome Localization of Gene Nod5 Controlling Nodulation in Pea, Dokl. Akad. Nauk, 1999, vol. 367, no. 6, pp. 851–852 [Dokl. (Engl. Transl.), vol. 367, no. 6, pp. 406–407].

    CAS  Google Scholar 

  • Sidorova, K.K., Shumnyi, V.K., and Nazaryuk, V.M., Simbioticheskaya azotfiksatsiya: geneticheskie, selektsionnye i ekologo-agrokhimicheskie aspekty (Symbiotic Nitrogen Fixation: Genetic, Breeding, Ecological and Agrochemical Aspects), Novosibirsk: Akad. Izd-vo “Geo”, 2006.

    Google Scholar 

  • Kholodar’, A.V., Shvetsov, S.V., and Chekurov, V.M., Application of Immune Analysis for Studies on Photoregulation of Gibberellin Level in Ethioplasts of Wheat Leaves, Fiziol. Rastenii, 1995, vol. 42, no. 4, pp. 647–651.

    Google Scholar 

  • Bhatia, C.R., Nichterlein, K., and Maluszynski, M., Mutations Affecting Nodulation in Grain Legumes and Their Potential in Sustainable Cropping Systems, Euphytica, 2001, vol. 120, pp. 415–432.

    Article  Google Scholar 

  • Duc, G. and Messager, A., Mutagenesis of Pea (Pisum sativum L.) and the Isolation of Mutants for Nodulation and Nitrogen Fixation, Plant Sci., 1989, vol. 60, pp. 207–213.

    Article  Google Scholar 

  • Fang, Y. and Hirsch, A.M., Studying Early Nodulin Gene ENOD40 Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa, Plant Physiol., 1998, vol. 116, pp. 53–68.

    Article  PubMed  CAS  Google Scholar 

  • Gresshoff, P.M., Molecular Genetic Analyses of Nodulation Genes in Soybean, Plant Breed. Rev., 1993, vol. 11, pp. 275–318.

    Google Scholar 

  • Herridge, D. and Rose, J., Breeding for Enhanced Nitrogen Fixation in Crop Legumes, Field Crops Res., 2000, vol. 65, pp. 229–248.

    Article  Google Scholar 

  • Jacobsen, E. and Nijdam, H.A., A Mutant Showing Efficient Nodulation in the Presence of Nitrate, Pisum Newslett., 1983, vol. 15. pp. 31–32.

    Google Scholar 

  • Kneen, B.E. and LaRue, T.A., Induced Symbiosis Mutants of Pea (Pisum sativum) and Sweet Clover (Melilotus alba annual), Plant Sci., 1988, vol. 58, pp. 177–182.

    Article  Google Scholar 

  • Mathesius, U., Djordjevic, M.A., Weinmann, J.J. et al., Transient Auxin Transport Inhibition and Localised Flavonoid Induction Occur During the Earliest Stages of Nodulation in White Clover, in Biological Nitrogen Fixation for the 21st Century, Elmerich, C., Condorosi, A., and Newton, W.E., Eds., Dordrecht: Kluwer, 1997.

    Google Scholar 

  • Postma, J.G., Jacobsen, E., and Feenstra, W.J., Three Pea Mutants with an Altered Nodulation Studied by Genetic Analysis and Grafting, J. Plant. Physiol., 1988, vol. 132, pp. 424–430.

    Google Scholar 

  • Sagan, M., Huguet, T., and Duc, G., Phenotypic Characterization and Classification of Nodulation Mutants of Pea (Pisum sativum L.), Plant Sci., 1994, vol. 100, pp. 59–70.

    Article  CAS  Google Scholar 

  • Sidorova, K.K., Shumny, V.K., Mischenko, T.M., and Vlasova, E.Yu., A New Gene for Supernodulation in Pea: nod6, Pisum Genet., 2003, vol. 35, pp. 28–29.

    Google Scholar 

  • Sidorova, K.K., Shumny, V.K., Vlasova, E.Yu. et al., Interaction of Two Symbiotic Genes within a Single Genotype, Pisum Genet., 2005a, vol. 37, pp. 34–36.

    Google Scholar 

  • Sidorova, K.K., Shumny, V.K., Vlasova, E.Yu. et al., A New Pea Symbiotic Mutant nod7, Pisum Genet., 2005b., vol. 37, pp. 37–38.

    Google Scholar 

  • Sidorova, K.K., Shumny, V.K., Vlasova, E.Yu. et al., The Brt (Branch Roots) and Lrt (Long Roots) Genes Control the Development of Roots in Peas (Pisum sativum L.), Pisum Genet., 2002, vol. 34, pp. 23–24.

    Google Scholar 

  • Sidorova, K.K., Shumny, V.K., Vlasova, E.Yu., and Mischenko, T.M., Dominant Symbiotic Pea Mutants, Pisum Genet., 2001, vol. 33, p. 35.

    Google Scholar 

  • Sidorova, K.K. and Uzhintseva L.P., Mapping of nod-4, a New Hypernodulating Mutant in Pea, Pisum Genet., 1995, vol. 27, p. 21.

    Google Scholar 

  • Sidorova, K.K., Vlasova, E.Yu., Mischenko, T.M. et al., A Study of Supernodulation Pea Mutants, Pisum Genet., 1999, vol. 31, p. 34.

    Google Scholar 

  • Song, L., Carroll, B.J., Gresshoff, P.M., and Herridge, D.F., Field Assessment of Supernodulating Genotypes of Soybean for Field, N2 Fixation and Benefit to Subseguent Crops, J. Soil Biol. Biochem., 1995, vol. 27, no. 4/5, pp. 563–569.

    Article  CAS  Google Scholar 

  • Stougaard, J., Regulators and Regulation of Legume Root Nodule Development, Plant Physiol., 2000, vol. 124, pp. 531–540.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    K. K. Sidorova, V. K. Shumny, E. Yu. Vlasova, M. N. Glyanenko, T. M. Mishchenko & G. G. Maystrenko

Authors
  1. K. K. Sidorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. K. Shumny
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Yu. Vlasova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. N. Glyanenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. M. Mishchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. G. Maystrenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. K. Sidorova.

Additional information

Original Russian Text © K.K. Sidorova, V.K. Shumny, E.Yu. Vlasova, M.N. Glyanenko, T.M. Mishchenko, G.G. Maystrenko, 2010, published in Informatsionnyi Vestnik Vavilovskogo Obshchestva Genetikov i Selektsionerov, 2010, Vol. 14, No. 2, pp. 357–374.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sidorova, K.K., Shumny, V.K., Vlasova, E.Y. et al. Symbiogenetics and breeding of a macrosymbiont for increased nitrogen fixation capacity with special reference to the pea (Pisum sativum L.). Russ J Genet Appl Res 1, 73–87 (2011). https://doi.org/10.1134/S2079059711010096

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079059711010096

Keywords

Search

Navigation