Abstract
A comparative study of the nerve structures and the density of mast cells in the heart of rats aged 3–4 and 23 months was carried out with histological and histochemical methods and the immunohistochemical markers PGP 9.5, tyrosine hydroxylase (TH), and synaptophysin. It was found that tissues innervation decreases with aging in all studied heart areas, particularly, sympathetic innervation. A decrease in sympathetic plexus density and the presence of dystrophic and degenerative changes in TH-immunopositive axon terminals were registered. It was also demonstrated that the mast cell population in old animals increases in the connective tissue of the pericardial, in the epicardium, and the myocardium of atria and ventricles region upon a decrease in sympathetic innervation. It is suggested that the increase in the number of mast cells in the heart during aging occurs in response to a deficiency in catecholamines and is related to the mast-cell secretion of biologically active substances (heparin, histamine, and serotonin), which help maintain local tissue homeostasis and adaptive and compensatory reactions of the animal heart.
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This work was carried out within the framework of the state assignment of the Federal State Budgetary Scientific Institution “Institute of Experimental Medicine.”
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Statement on the welfare of animals. The work was conducted in compliance with the EU legislation on the protection of animals used for scientific purposes and the Rules for Researches with Experimental Animals (the application for the order by Health Ministry of USSR on August 12, 1977, no. 755). The study was approved by local ethical committee of the Institute of Experimental Medicine in St. Petersburg (protocol no. 3 from November 30, 2017).
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Chumasov, E.I., Petrova, E.S., Kolos, E.A. et al. Study of the Nerve Apparatus and Mast Cells in the Hearts of Old Rats. Adv Gerontol 11, 29–36 (2021). https://doi.org/10.1134/S2079057021010355
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DOI: https://doi.org/10.1134/S2079057021010355