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Detection of the Causative Agent of Tularemia by Surface Plasmon Resonance

  • INVESTIGATION METHODS FOR PHYSICOCHEMICAL SYSTEMS
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Abstract

A method for detection of causative agent of tularemia in aqueous solutions by surface plasmon resonance spectrometry was proposed. The kinetics of immobilization of antibodies to Francisella tularensis on the surface of gold-plated glass chips was studied. Based on a quantitative analysis of the results, it was concluded that the antibody molecules were predominantly laterally oriented on the surface. This conclusion was confirmed by analysis of sensorgrams of the subsequent antigen–antibody interaction. It is shown that the proposed method can reliably determine the presence of the causative agent of tularemia in aqueous solutions with a sensitivity of 1.0 × 102 cell/mL for several minutes. Various options for improving this method for detection of the tularemia causative agent to increase its sensitivity have been proposed.

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FUNDING

The work was performed in the framework of a Scientific and Technical Agreement of September 15, 2015, between the Institute of Physical Chemistry of the Russian Academy of Sciences and the Stavropol Research Antiplague Institute on a nonreimbursable basis.

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Authors and Affiliations

  1. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071, Moscow, Russia

    A. A. Isakova & A. V. Indenbom

  2. Stavropol Research Antiplague Institute, 355106, Stavropol, Russia

    I. V. Zharnikova & T. V. Zharnikova

  3. Moscow Institute of Physics and Technology (State University), 141701, Dolgoprudnyi, Moscow oblast, Russia

    A. V. Indenbom

Authors
  1. A. A. Isakova
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  2. I. V. Zharnikova
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  3. T. V. Zharnikova
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  4. A. V. Indenbom
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Corresponding authors

Correspondence to A. A. Isakova or A. V. Indenbom.

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Translated by A. Bulaev

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Isakova, A.A., Zharnikova, I.V., Zharnikova, T.V. et al. Detection of the Causative Agent of Tularemia by Surface Plasmon Resonance. Prot Met Phys Chem Surf 55, 407–411 (2019). https://doi.org/10.1134/S2070205119020102

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  • DOI: https://doi.org/10.1134/S2070205119020102

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