Abstract
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agentFrancisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detectF. tularensis with the limit of detection 105 CFU/mL with a typical measuring cycle >5 min. The sensor was successfully evaluated for rapid detection ofF. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
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Abbreviations
- BTA:
-
biothreat alert
- BWA:
-
biological warfare agent
- ELISA:
-
enzyme-linked immunosorbent assay
- LD50 :
-
lethal dose
- LOD:
-
limit of detection
- LPS:
-
lipopolysaccharide
- PBS:
-
phosphate-buffered saline
- PCR:
-
polymerase chain reaction
- PZ:
-
piezoelectric
- QCM:
-
quartz crystal microbalance
- RAPID:
-
ruggedized advanced pathogen identification device
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Pohanka, M., Skládal, P. Piezoelectric immunosensor for the direct and rapid detection ofFrancisella tularensis . Folia Microbiol 52, 325–330 (2007). https://doi.org/10.1007/BF02932086
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DOI: https://doi.org/10.1007/BF02932086