Abstract
The heterogeneous BCL biocatalyst based on the recombinant extracellular lipase from the thermophilic bacteria Geobacillus stearothermophilus G3 with an activity of 23.6 U.A./g was prepared by covalent immobilization on aminated silica gel. The effect of the solvent, temperature (30–60°C), methanol : oil molar ratio (1 : 1 to 9 : 1), and the amounts of water (1–10%) and catalyst (0.25–25%) on the yield of fatty acid methyl esters (FAME) during the methanolysis of sunflower oil with BCL was studied. The maximum yield of FAME was 43%. The biocatalyst exhibits high operational stability: after 480 h of operation (20 cycles), it retains more than 50% of its original activity, making BCL a promising catalyst for application in manufacturing of FAME as feedstock for biodiesel production.
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Moser, B.R., In vitro Cell Dev. Biol.: Plant, 2009, vol. 45, no. 3, pp. 229–266.
Demirbas, A., Appl. Energy, 2009, vol. 86, suppl. 1, pp. S108–S117.
Baroutian, S., Aroua, M.K., Raman, A.A.A., and Sulaiman, N.M.N., J. Chem. Eng. Data, 2008, vol. 53, no. 3, pp. 877–880.
Di Serio, M., Tesser, R., Pengmei, L., and Santacesaria, E., Energy Fuels, 2008, vol. 22, no. 1, pp. 207–217.
Tan, T., Lu, J., Nie, K., Deng, L., and Wang, F., Biotechnol. Adv., 2010, vol. 28, no. 5, pp. 628–634.
Samoylova, Yu.V., Tulupov, A.A., and Sorokina, K.N., Vestn. Novosib. Gos. Univ., Ser. Biol. Klin. Med., 2014, vol. 12, no. 4, pp. 77–84.
Huynh, L.H., Kasim, N.S., and Ju, Y.H., in Biofuels: Alternative Feedstocks and Conversion Processes, Pandey, A., Laeeoche, C., Ricke, S.C., Dussap, C.-G., and Gnansounou, E., Eds., Amsterdam: Elsevier, 2011, pp. 375–392.
Chen, L., Liu, T., Zhang, W., Chen, X., and Wang, J., Bioresour. Technol., 2012, vol. 111, pp. 208–214.
Fjerbaek, L., Christensen, K.V., and Norddahl, B., Biotechnol. Bioeng., 2009, vol. 102, no. 5, pp. 1298–1315.
Shimada, Y., Watanabe, Y., Samukawa, T., Sugihara, A., Noda, H., Fukuda, H., and Tominaga, Y., J. Am. Oil Chem. Soc., 1999, vol. 76, no. 7, pp. 789–793.
Xu, Y., Du, W., Zeng, J., and Liu, D., Biocatal. Biotransform., 2004, vol. 22, no. 1, pp. 45–48.
Rodrigues, R.C. and Fernandez-Lafuente, R., J. Mol. Catal. B: Enzym., 2010, vol. 64, nos. 1–2, pp. 1–22.
Al’myasheva, N.R., Kopitsyn, D.S., Vinokurov, A.A., and Novikov, A.A., Chem. Technol. Fuels Oils, 2015, vol. 50, no. 6, pp. 449–452.
Da Rós, P.C.M., Silva, G.A.M., Mendes, A.A., Santos, J.C., and de Castro, H.F., Bioresour. Technol., 2010, vol. 101, no. 14, pp. 5508–5516.
Dror, A., Shemesh, E., Dayan, N., and Fishman, A., Appl. Environ. Microbiol., 2014, vol. 80, no. 4, pp. 1515–1527.
Ebrahimpour, A., Rahman, R.N.Z.R.A., Barsi, M., and Salleh, A.B., Bioresour. Technol., 2011, vol. 102, no. 13, pp. 6972–6981.
Sorokina, K.N., Nuriddinov, M.A., Rozanov, A.S., Ivanisenko, V.A., and Pel’tek, S.E., Vavilov. Zh. Genet. Sel., 2013, vol. 17, no. 4/1, pp. 666–674.
Sorokina, K.N., Rozanov, A.S., Bryanskaya, A.V., and Pel’tek, S.E., Vavilov. Zh. Genet. Sel., 2013, vol. 17, no. 4/1, pp. 651–658.
Bradford, M.M., Anal. Biochem., 1976, vol. 72, nos. 1–2, pp. 248–254.
JIS K (Japanese Industrial Standard) 0070-1992: Test Method for Acidity, Saponification Value, Ester Value, Iodine Value and Hydroxy Value of Chemical Products and Unsaponifiables, 1992.
Costa, S.A., Azevedo, H.S., and Reis, R.L., in Biodegradable Systems in Tissue Engineering and Regenerative Medicine, Reis, R.L. and San Román, J., Eds., Boca Raton, FL: CRC Press, 2004, pp. 301–318.
Narwal, S.K., Saun, N.K., Dogra, P., Chauhan, G., and Gupta, R., BioMed. Res. Int., 2015. http://dxdoi. org/10.1155/2015/281934. Cited May 21, 2016.
Handbook of Plant-Based Biofuels, Pandey, A., Ed., Boca Raton, FL: CRC Press, 2009.
Li, W., Du, W., Liu, D., and Yao, Y., Biochem. Eng. J., 2008, vol. 41, no. 2, pp. 111–115.
Du, W., Xu, Y., Liu, D., and Zeng, J., J. Mol. Catal. B: Enzym., 2004, vol. 30, nos. 3–4, pp. 125–129.
Da Silva, C. and Oliveira, J.V., Braz. J. Chem. Eng., 2014, vol. 31, no. 2, pp. 271–285. http://dxdoiorg/ 10.1590/0104-6632.20140312s00002616. Cited May 21, 2016.
Patel, U., Vaghela, A., and Patel, N.M., Int. J. Res. Inf. Technol., 2013, vol. 1, no. 4, pp. 17–30.
Lukovic, N., Kneževic-Jugovic, Z., and Bezbradica, D., in Alternative Fuel, Manzanera, M., Ed., Rijeka: InTech, 2011, ch. 3, pp. 47–72.
Liu, Y., Xin, H.-L., and Yan, Y.-J., Ind. Crops Prod., 2009, vol. 30, no. 3, pp. 431–436.
Wang, L., Du, W., Liu, D., Li, L., and Dai, N., J. Mol. Catal. B: Enzym., 2006, vol. 43, nos. 1–4, pp. 29–32.
Lu, J., Nie, K., Xie, F., Wang, F., and Tan, T., Process Biochem., 2007, vol. 42, no. 9, pp. 1367–1370.
Salis, A., Pinna, M., Monduzzi, M., and Solinas, V., J. Biotechnol., 2005, vol. 119, no. 3, pp. 291–299.
Zheng, Y., Quan, J., Ning, X., Zhu, L.-M., Jiang, B., and He, Z.-Y., World J. Microbiol. Biotechnol., 2009, vol. 25, no. 1, pp. 41–46.
Noureddini, H., Gao, X., and Philkana, R.S., Bioresour. Technol., 2005, vol. 96, no. 7, pp. 769–777.
Ognjanovic, N., Bezbradica, D., and Knezevic-Jugovic, Z., Bioresour. Technol., 2009, vol. 100, no. 21, pp. 5146–5154.
Kumari, V., Shah, S., and Gupta, M.N., Energy Fuels, 2007, vol. 21, no. 1, pp. 368–372.
Xie, W. and Ma, N., Energy Fuels, 2009, vol. 23, no. 3, pp. 1347–1353.
Kawakami, K., Oda, Y., and Takahashi, R., Biotechnol. Biofuels, 2011, vol. 4, no. 1. doi 10.1186/1754-6834-4-42
Yang, G., Wu, J., Xu, G., and Yang, L., Colloids Surf., B, 2010, vol. 78, no. 2, pp. 351–356.
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Original Russian Text © Y.V. Samoylova, A.V. Piligaev, K.N. Sorokina, A.S. Rozanov, S.E. Peltek, A.A. Novikov, N.R. Almyasheva, V.N. Parmon, 2015, published in Kataliz v Promyshlennosti.
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Samoylova, Y.V., Piligaev, A.V., Sorokina, K.N. et al. Application of the immobilized bacterial recombinant lipase from Geobacillus stearothermophilus G3 for the production of fatty acid methyl esters. Catal. Ind. 8, 187–193 (2016). https://doi.org/10.1134/S2070050416020082
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DOI: https://doi.org/10.1134/S2070050416020082