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Investigation of micro- and nanostructure of biocompatible scaffolds from regenerated fibroin of Bombix mori by scanning probe nanotomography

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Abstract

This paper presents a study of three-dimensional micro- and nanosctuctures of porous biocompatible matrices from regenerated fibroin and a quantitative analysis of their microporosity parameters. An analysis of the three-dimensional structure of matrices has been carried out by scanning probe nanotomography with the use of an experimental setup combining an ultramicrotome and scanning probe microscope. The formation of a three-dimensional network of interconnected pores with characteristic dimensions ranging from 1.7 to 6.0 μm is observed in the bulk volume of studied matrices. The measured mean pore diameter is 3.54 ± 1.23 μm; the mean pore wall thickness is 672 ± 282 nm. The volume porosity of macropore walls is 65.7%, while the volume fraction of pores interconnected in large pore clusters is more than 80% of the whole pore volume. Quantitative characteristics of porous micro- and nanostructures of matrices obtained as a result of the study show a significant degree of porosity and percolation of micropores, which correlates with the reported high efficiency of tissue regeneration on such matrices. The use of scanning probe nanotomography to analyze three-dimensional morphology characteristics and the topology of micro- and nanopore systems enables us to improve the efficiency of developing new biomaterials.

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Authors and Affiliations

  1. Laboratory of Bionanotechnology, V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, 123182, Russia

    A. E. Efimov & I. I. Agapov

  2. Faculty of Biology, Moscow State University, Moscow, 119991, Russia

    M. M. Moisenovich, A. G. Kuznetsov, L. A. Safonova & M. M. Bobrova

Authors
  1. A. E. Efimov
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  2. M. M. Moisenovich
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  3. A. G. Kuznetsov
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  4. L. A. Safonova
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  5. M. M. Bobrova
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  6. I. I. Agapov
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Correspondence to I. I. Agapov.

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Original Russian Text © A.E. Efimov, M.M. Moisenovich, A.G. Kuznetsov, L.A. Safonova, M.M. Bobrova, I.I. Agapov, 2014, published in Rossiiskie Nanotekhnologii, 2014, Vol. 9, Nos. 11–12.

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Efimov, A.E., Moisenovich, M.M., Kuznetsov, A.G. et al. Investigation of micro- and nanostructure of biocompatible scaffolds from regenerated fibroin of Bombix mori by scanning probe nanotomography. Nanotechnol Russia 9, 688–692 (2014). https://doi.org/10.1134/S1995078014060081

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  • DOI: https://doi.org/10.1134/S1995078014060081

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