Abstract
Pre-selection of tumor exosomes from a total pool of circulating microvesicles is an important stage required to increase sensitivity and specificity of the developing methods for diagnostics of oncological diseases. In this study, universal proteins characterized by increased expression in plasma exosomes have been identified in patients with colorectal cancer, head and neck squamous cell carcinomas, and lung cancer. The use of antibodies against major exosomal proteins will help to develop a simple and high-performance method of affinity isolation of tumor exosomes.
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Jemal, A., Vineis, P., Bray, F., Torre, L., and Forman, D., The Cancer Atlas, 2nd ed., Atlanta, GA: American Cancer Society, 2014.
Tamkovich, S.N., Voitsitskii, V.E., and Laktionov, P.P., Biomed. Khim., 2014, vol. 60, pp. 141–160.
Choi, D.S., Kim, D.K., Kim, Y.K., and Gho, Y.S., Proteomics, 2013, vol. 13, pp. 1554–1571. doi 10.1002/pmic.201200329
Sempere, L.F., Keto, J., and Fabbri, M., Cancers (Basel), 2017, vol. 9, 71. doi 10.3390/cancers9070071
Boukouris, S. and Mathivanan, S., Proteomics Clin. Appl., 2015, vol. 9, pp. 358–367. doi 10.1002/prca.201400114
Clark, D.J., Fondrie, W.E., Liao, Z., Hanson, P.I., Fulton, A., Mao, L., and Yang, A.J., Anal. Chem., 2015, vol. 87, pp. 10462–10469. doi 10.1021/acs.analchem. 5b02586
Tamkovich, S.N., Yunusova, N.V., Stakheeva, M.N., Somov, A.K., Frolova, A.E., Kirushina, N.A., Afanasyev, S.G., Grigoryeva, A.E., Laktionov, P.P., and Kondakova, I.V., Biomed. Khim., 2017, vol. 63, pp. 165–169. doi 10.18097/PBMC20176302165
Gaál, Ö., Medgyesi, G., and Vereczkey, L., Elektroforez v razdelenii biologicheskikh makromolekul (Electrophoresis in Separation of Biological Macromolecules), Moscow: Mir, 1982.
Lötvall, J., Hill, A.F., Hochberg, F., Buzás, E.I., Di Vizio, D., Gardiner, C., Gho, Y.S., Kurochkin, I.V., Mathivanan, S., Quesenberry, P., Sahoo, S., Tahara, H., Wauben, M.H., Witwer, K.W., and Théry, C., J. Extracell. Vesicles, 2014, vol. 3, 26913. doi 10.3402/jev.v3.26913
Lässer, C., Alikhani, V.S., Ekström, K., Eldh, M., Paredes, P.T., Bossios, A., Sjöstrand, M., Gabrielsson, S., Lötvall, J., and Valadi, H., J. Transl. Med., 2011, vol. 9, 9. doi 10.1186/1479-5876-9-9
Théry, C., Amigorena, S., and Raposo, G., Curr. Protoc. Cell Biol., 2006, Chapter 3.22, pp. 3.22.1−3.22.29.
Grigor’eva, A.E., Tamkovich, S.N., Eremina, A.V., Tupikin, A.E., Kabilov, M.R., Chernykh, V.V., Vlassov, V.V., Laktionov, P.P., and Ryabchikova, E.I., Biomed. Khim., 2016, vol. 62, pp. 99–106. doi 10.18097/PBMC20166201099
Samsonov, R., Shtam, T., Burdakov, V., Glotov, A., Tsyrlina, E., Berstein, L., Nosov, A., Evtushenko, V., Filatov, M., and Malek, A., Prostate, 2016, vol. 76, pp. 68–79. doi 10.1002/pros.23101
Andreu, Z. and Yanez-Mo, M., Front. Immunol., 2014, vol. 5, 442. doi 10.3389/fimmu.2014.00442
King, J.B., von Furstenberg, R.J., Smith, B.J., McNaughton, K.K., Galanko, J.A., and Henning, C.J., Am. J. Physiol. Gastrointest. Liver Physiol., 2012, vol. 303, pp. G443–G452. doi 10.1152/ajpgi.00087.2012
Palazzolo, G., Albanese, N.N., Gianluca, D.C., Gygax, D., Vittorelli, M.L., and Pucci-Minafram, I., Anticancer Res., 2012, vol. 32, pp. 847–860.
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Original Russian Text © S.N. Tamkovich, N.V. Yunusova, A.K. Somov, S.G. Afanas’ev, G.V. Kakurina, E.S. Kolegova, E.A. Tugutova, P.P. Laktionov, I.V. Kondakova, 2018, published in Biomeditsinskaya Khimiya.
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Tamkovich, S.N., Yunusova, N.V., Somov, A.K. et al. Comparative Subpopulation Analysis of Plasma Exosomes from Cancer Patients. Biochem. Moscow Suppl. Ser. B 12, 151–155 (2018). https://doi.org/10.1134/S1990750818020130
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DOI: https://doi.org/10.1134/S1990750818020130