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Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

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Abstract

A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Akad. Lavrentieva 8, Novosibirsk, 630090, Russia

    S. N. Tamkovich, A. K. Somov, A. E. Grigor’eva & P. P. Laktionov

  2. Tomsk Cancer Research Institute, Tomsk, Russia

    N. V. Yunusova, M. N. Stakheeva, A. E. Frolova, S. G. Afanasyev & I. V. Kondakova

  3. Novosibirsk State University, Novosibirsk, Russia

    S. N. Tamkovich

  4. Novosibirsk Regional Oncological Dispensary, Novosibirsk, Russia

    N. A. Kiryushina

Authors
  1. S. N. Tamkovich
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  2. N. V. Yunusova
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  3. M. N. Stakheeva
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  4. A. K. Somov
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  5. A. E. Frolova
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  6. N. A. Kiryushina
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  7. S. G. Afanasyev
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  8. A. E. Grigor’eva
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  9. P. P. Laktionov
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  10. I. V. Kondakova
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Corresponding author

Correspondence to S. N. Tamkovich.

Additional information

Original Russian Text © S.N. Tamkovich, N.V. Yunusova, M.N. Stakheeva, A.K. Somov, A.E. Frolova, N.A. Kiryushina, S.G. Afanasyev, A.E. Grigor’eva, P.P. Laktionov, I.V. Kondakova, 2017, published in Biomeditsinskaya Khimiya.

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Tamkovich, S.N., Yunusova, N.V., Stakheeva, M.N. et al. Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. Biochem. Moscow Suppl. Ser. B 11, 291–295 (2017). https://doi.org/10.1134/S1990750817030106

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  • DOI: https://doi.org/10.1134/S1990750817030106

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