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Possibility of Application of Extracellular Protease of the Micromycetе Aspergillus ochraceus VKM F-4104D for Determination of the Protein C Content in Human Blood Plasma

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Abstract

The protein C activator activity, determined in normal plasma by using A. ochraceus protease is comparable with the activity of a commercial protease analogue from the South American copperhead venom (Protac®). As in the case of Protac®, the A. ochraceus protease can be used for protein C determination in plasma with its reduced content. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may be a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.

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Authors and Affiliations

  1. Biological Faculty of Moscow State University, Moscow, 119234, Russia

    A. A. Osmolovskiy, A. V. Orekhova, V. G. Kreyer & N. A. Baranova

  2. Faculty of Fundamental Medicine of Moscow State University, Moscow, Russia

    A. V. Orekhova

  3. International Biotechnology Center of Moscow State University, Moscow, Russia

    N. S. Egorov

Authors
  1. A. A. Osmolovskiy
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  2. A. V. Orekhova
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  3. V. G. Kreyer
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  4. N. A. Baranova
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  5. N. S. Egorov
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Correspondence to A. A. Osmolovskiy.

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Original Russian Text © A.A. Osmolovskiy, A.V. Orekhova, V.G. Kreyer, N.A. Baranova, N.S. Egorov, 2018, published in Biomeditsinskaya Khimiya.

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Osmolovskiy, A.A., Orekhova, A.V., Kreyer, V.G. et al. Possibility of Application of Extracellular Protease of the Micromycetе Aspergillus ochraceus VKM F-4104D for Determination of the Protein C Content in Human Blood Plasma. Biochem. Moscow Suppl. Ser. B 12, 164–166 (2018). https://doi.org/10.1134/S1990750818020099

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  • DOI: https://doi.org/10.1134/S1990750818020099

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