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Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma

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Abstract

It has been shown that factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity determined with a commercial analogue, a protease from Russell’s viper venom (RVV-X®). It was revealed that the protease of A. ochraceus, along with the RVV-X® preparation, can be used to determine the content of factor X in the plasma with its reduced content. A study of the protease activity of A. ochraceus as an activator to factor X showed that it is slightly higher compared to the snake venom preparation, which can make A. ochraceus protease a promising substitute for the snake activator in diagnostics for determining the content of factor X.

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REFERENCES

  1. Girolami, A., Molaro, G., and De Marco, L., Factor X survival and therapeutic factor X levels in the abnormal factor X (factor X Friuli) coagulation disorder, Acta Haematol., 1974, vol. 52, no. 4, pp. 223–231.

    Article  CAS  PubMed  Google Scholar 

  2. Chatterjee, T., Philip, J., Nair, V., Mallhi, R.S., Sharma, H., Ganguly, P., and Biswas, A.K., Inherited factor X (Stuart-Prower factor) deficiency and its management, Med. J. Armed Forces India, 2015, vol. 71, suppl. 1, pp. S184–S186.

    Article  CAS  PubMed  Google Scholar 

  3. Tsevrenis, H., Mandalaki, T., Symvoulidis, A., and Philips, H., Fluctuations and relations of the Quick time and the heparin tolerance test and the X factor during anticoagulant treatment with coumarins, Arch. Inst. Pasteur Hell., 1961, vol. 7, pp. 49–58.

    CAS  PubMed  Google Scholar 

  4. Sajevic, T., Leonardi, A., and Križaj, I., Haemostatically active proteins in snake venoms, Toxicon, 2011, vol. 57, no. 5, pp. 627–645.

    Article  CAS  PubMed  Google Scholar 

  5. Bobrovskaya, A.A., Osmolovskii, A.A., Zvonareva, E.S., Kreier, V.G., and Kurakov, A.V., Proteinases of micromycetes with the activities of human hemostasis enzymes, Usp. Med. Mikol., 2015, vol. 14, no. 14, pp. 414–416.

    Google Scholar 

  6. Osmolovskiy, A.A., Orekhova, A.V., Kreyer, V.G., Baranova, N.A., and Egorov, N.S., Possibility of application of extracellular proteases of the micromycete Aspergillus ochraceus VKM F-4104D for determination of the protein C content in human blood plasma, Biochem. (Moscow) Suppl. Ser. B, Biomed. Chem., 2018, vol. 12, no. 2, pp. 164–166.

    Google Scholar 

  7. Osmolovsky, A.A., Kreier, V.G., Baranova, N.A., Kurakov, A.V., and Egorov, N.S., Production of extracellular proteinases—protein C activators of blood plasma—by the micromycete Aspergillus ochraceus during submerged and solid-state fermentation, Appl. Biochem. Microbiol., 2013, vol. 49, no. 6, pp. 581–586.

    Article  CAS  Google Scholar 

  8. Barranco-Medina, S., Murphy, M., Pelc, L., Chen, Z., Di Cera, E., and Pozzi, N., Rational design of protein C activators, Sci. Rep., 2017, vol. 7, p. 44596.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Gertler, A. and Trop, M., The elastase-like enzymes from Streptomyces griseus (pronase). Isolation and partial characterization, Eur. J. Biochem., 1971, vol. 19, no. 1, pp. 90–96.

    Article  CAS  PubMed  Google Scholar 

  10. Osmolovskiy, A.A., Kreier, V.G., Kurakov, A.V., Baranova, N.A., and Egorov, N.S., Aspergillus ochraceus micromycetes—producers of extracellular proteinases—protein C activators of blood plasma, Appl. Biochem. Microbiol., 2012, vol. 48, no. 5, pp. 488–492.

    Article  CAS  Google Scholar 

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Funding

This work was supported by the Foundation for the Promotion of the Development of Small Enterprises in the Scientific and Technical Sphere (agreement no. 9107GU / 2015).

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Authors and Affiliations

  1. Department of Microbiology, Biological Faculty, Moscow State University, 119234, Moscow, Russia

    A. V. Orekhova, A. A. Osmolovskiy, V. G. Kreyer & N. A. Baranova

  2. International Biotechnology Center, Moscow State University, 119234, Moscow, Russia

    N. S. Egorov

Authors
  1. A. V. Orekhova
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  2. A. A. Osmolovskiy
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  3. V. G. Kreyer
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  4. N. A. Baranova
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  5. N. S. Egorov
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Corresponding author

Correspondence to A. A. Osmolovskiy.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. The studies were performed without the use of animals.

Statement of compliance with standards of research involving humans as subjects. The studies did not involve people as subjects.

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Translated by I. Shipounova

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Orekhova, A.V., Osmolovskiy, A.A., Kreyer, V.G. et al. Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma. Moscow Univ. Biol.Sci. Bull. 74, 117–120 (2019). https://doi.org/10.3103/S0096392519020081

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  • DOI: https://doi.org/10.3103/S0096392519020081

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