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Construction and Characterization of a Recombinant Mutant Homolog of the CheW Protein from Thermotoga petrophila RKU-1

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Abstract

In this work, a recombinant chemotaxis CheW protein from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homologue (TpeCheW-mut) have been obtained. Despite the low homology with the CheW protein of the Escherichia coli bacteria, these proteins do not cause metabolic overload and are well expressed by E. coli laboratory strains. A wide range of features and parameters important for isolation of the TpeCheW-mut protein, such as stability over a wide range of temperatures and pH values, high level of expression, solubility, and the possibility of using simple low-stage purification schemes, including heat pretreatment, has been recognized. Possible directions of using this protein in the practice of scientific and applied research have been formulated and justified.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, ul. Pogodinskaya 10, Moscow, 119121, Russia

    D. V. Grishin, D. D. Zhdanov, Ju. A. Gladilina, V. S. Pokrovsky, O. V. Podobed, M. V. Pokrovskaya, S. S. Aleksandrova, A. L. Milyushkina, M. A. Vigovskiy & N. N. Sokolov

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  1. D. V. Grishin
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  2. D. D. Zhdanov
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  3. Ju. A. Gladilina
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  4. V. S. Pokrovsky
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  5. O. V. Podobed
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  6. M. V. Pokrovskaya
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  7. S. S. Aleksandrova
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Correspondence to D. V. Grishin.

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Original Russian Text © D.V. Grishin, D.D. Zhdanov, Ju.A. Gladilina, V.S. Pokrovsky, O.V. Podobed, M.V. Pokrovskaya, S.S. Aleksandrova, A.L. Milyushkina, M.A. Vigovskiy, N.N. Sokolov, 2018, published in Biomeditsinskaya Khimiya.

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Grishin, D.V., Zhdanov, D.D., Gladilina, J.A. et al. Construction and Characterization of a Recombinant Mutant Homolog of the CheW Protein from Thermotoga petrophila RKU-1. Biochem. Moscow Suppl. Ser. B 12, 143–150 (2018). https://doi.org/10.1134/S1990750818020051

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  • DOI: https://doi.org/10.1134/S1990750818020051

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