Abstract
The effect of structurally different sulfated polysaccharides from brown algae Fucus evanescens, Saccharina cichorioides, and Saccharina japonica on the morphofunctional changes of dendritic cells has been studied using flow cytometry and phase-contrast microscopy. It has been found that the dendritic cells are characterized by larger sizes, vacuolated cytoplasm, eccentrically located nucleus, the presence of numerous cytoplasmic pseudopodia of various forms; they do express surface markers, thus providing evidence for their maturation (CD83, CD11c, HLA-DR, CD86). Increased production of immunoregulatory (IL-12) and proinflammatory cytokines (TNF-α, IL-6) by dendritic cells polarizes the development of the Th-1 type immune response.
Similar content being viewed by others
References
Akhmatova, N.K. and Kiselevskii, M.V., Vrozhdennyi immunitet protivoopukholevyi i protivoinfektsionnyi (Antitumor and Antiinfective Innate Immunity), Moscow Prakticheskaya Meditsina, 2008.
Talaev, V.Yu., Plekhanova, M.V., and Matveichev, A.V., Medial, 2014, vol. 2, p. 135–153.
Kremer, E.E., Cellular mechanisms of the immune response induced by the Opisthorchis felineus extract in bronchial asthma, Cand. Sci. (Med. Sci.), Tomsk Siberian State Medical University, 2012.
Anastyuk, S.D. and Zvyagintseva, T.N., in Fucoidans–sulfatirovannye polysakharidy burykh vodoroslei. Struktura. Fermentativnaya transformatsiya i biologicheskie svoistva (Fucoidans—Sulfated Polysaccharides from Brown Algae. Structure. Enzymatic transformation and Biological Features), Besednova N.N. and Zvyagintseva T.N., Eds., Vladivostok: Dal’nauka, FEB RAS, 2014, pp. 60–95.
Kim, S.-Y. and Joo, H.-G., J. Vet. Sci., 2015, vol. 16, pp. 145–150. doi 10.4142/jvs.2015.16.2.145
Fitton, J. H., Stringer, D.N., and Karpiniec, S.S., Mar. Drugs, 2015, vol. 13, pp. 5920–5946. doi 10.3390/md13095920
Lebedynskaya, E.A., Makarenkova, I.D., Lebedynskaya, O.V., Akhmatova, N.K., and Zvyagintseva, TN., Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2015, vol. 9, pp. 86–94.
Zvyagintseva, T.N., Shevchenko, N.M., Chizhov, A.O., Krupnova, T.N., Sundukova, E.V., and Isakov, V.V., J. Exp. Mar. Biol. Ecol., 2003, vol. 294, pp. 1–13.
Makarenkova, I.D., Logunov, D.Yu., Tukhvatulin, A.I., Semenova, I.B., Zvyagintseva, T.N., Gorbach, V.I., Ermakova, S.P., and Besednova, N.N., Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2012, vol. 6, no. 1, pp. 75–80.
Wang, W., Wang, S.X., and Guan, H.S., Mar. Drugs, 2012, vol. 10, pp. 2795–2816.
Vishchuk, O.S., Tarbeeva, D.V., Ermakova, S.P., and Zvyagintseva, T.N., Chem. Biodivers., 2012, vol. 9, pp. 817–828.
Fang, Q., Wang, J.-F., Zha, X.-Q., Cui, S.-H., Cao L., and Luo, J.-P., Carbohydr. Polym., 2015, vol. 134, pp. 66–73.
Meng, J., Cao, Y., Meng, Y., Luo, H., Gao, X., and Shan, F., Int. J. Biol. Macromol., 2014, vol. 69, pp. 388–392.
Lin, C.C., Pan, I.H., Li, Y.R., Pan, Y.G., Lin, M.K., Lu, Y.H., Wu, H.C, and Chu, C.L., PLoS One, 2015, vol. 10, no. 2, e0116191. doi 10.1371/journal. pone.0116191
Chkadua, G.Z., Zabotina, T.N., Burkova, A.A., Tamaeva, Z.E., Ogorodnikova, E.V., Zhordania, K.I., Kadagidze, Z.G., and Baryshnikov, A.Yu., Eksper. Bioter., 2002, vol. 1, pp. 56–62.
Boks, M.A., Kager-Groenland, J.R., Haasjes, M.S., Zwaginga, J.J., van Ham, S.M., and ten Brinke, A., Clin. Immunol., 2012, vol. 142, pp. 332–342.
Khil’chenko, S.R., Zaporozhets, T.S., Shevchenko, N.M., Zvyagintseva, T.N., Vogel, U., Seeberger, P., and Lepenies, B., Journal of Carbohydrate Chemistry, 2011, vol. 30, pp. 291–305.
Besednova, N.N. and Zaporozhets, T.S., Zhurn. Mikrobiol. Epidemiol. Immunobiol., 2011, vol. 5, pp. 98–106.
Teruya, T., Takeda, S., Tamaki, Y., and Tako, M., Bioscience, Biotechnology and Biochemistry, 2010, vol. 4, pp. 1960–1962.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © I.D. Makarenkova, N.K. Akhmatova, S.P. Ermakova, N.N. Besednova, 2017, published in Biomeditsinskaya Khimiya.
Rights and permissions
About this article
Cite this article
Makarenkova, I.D., Akhmatova, N.K., Ermakova, S.P. et al. Morphofunctional changes of dendritic cells induced by sulfated polysaccharides of brown algae. Biochem. Moscow Suppl. Ser. B 11, 243–250 (2017). https://doi.org/10.1134/S1990750817030076
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1990750817030076