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The combined action of binase and bleomycin on human lung adenocarcinoma cells

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Abstract

Some microbial ribonucleases (RNases) demonstrate selective cytotoxic effect against a wide range of tumor cells. In this context combined use of cytotoxic RNases in complex therapy with other chemotherapeutic agents appears to be especially promising. In this study we have investigated the apoptosisinduced effect of Bacillus pumilus RNase (binase) in combination with known antitumor antibiotic bleomycin on human lung adenocarcinoma A549 cells. The combined effect of high concentrations of these agents did not have any mutual increase in their apoptosisinduced action, while a combination of nonapoptotic concentrations resulted in the increase of proportion of apoptotic cells up to 22% as compared with individual effect of bleomycin (6%) and binase (12%) used separately. These results indicate that binase and bleomycin are effective in combination of their low concentrations and ineffective in combination of their high concentrations.

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Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, ul. Kremlyovskaya 18, Kazan, 420008, Russia

    P. V. Zelenikhin, A. V. Makeeva, Y. A. Siraj & O. N. Ilinskaya

  2. College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, 79, Ethiopia

    T. N. Nguen & Y. A. Siraj

Authors
  1. P. V. Zelenikhin
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  2. A. V. Makeeva
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  3. T. N. Nguen
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  4. Y. A. Siraj
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  5. O. N. Ilinskaya
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Correspondence to P. V. Zelenikhin.

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Original Russian Text © P.V. Zelenikhin, A.V. Makeeva, T.N. Nguen, Y.A. Siraj, O.N. Ilinskaya, 2016, published in Biomeditsinskaya Khimiya.

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Zelenikhin, P.V., Makeeva, A.V., Nguen, T.N. et al. The combined action of binase and bleomycin on human lung adenocarcinoma cells. Biochem. Moscow Suppl. Ser. B 10, 87–90 (2016). https://doi.org/10.1134/S1990750816010121

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  • DOI: https://doi.org/10.1134/S1990750816010121

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