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Binase-Induced Changes of Tumor Cell Membranes

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Abstract

Exogenous ribonucleases of Bacilli can selectively induce apoptosis of malignant cells. The ability of Bacillus pumilus ribonuclease, binase, to induce processes leading to a dynamic disruption of the integrity of A549 human pulmonary adenocarcinoma cell membranes was analyzed. The influence of different enzyme concentrations on the state of the cytoplasmic membrane of cells and mitochondrial membranes was characterized. Using the methods of flow cytofluorometry and fluorescence microscopy, it has been established that binase leads to disruption in normal functioning of both types of membranes, with mitochondrial membranes affected first. The study made it possible to identify and visualize the effects of binase on the membrane structures of target cells and to confirm that bacterial RNase induces apoptosis of target cells mainly through the “internal” (mitochondrial) pathway.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, 420008, Russia

    P. V. Zelenikhin, A. S. Gorbunova, A. V. Makeeva & O. N. Ilinskaya

  2. Rudolf Buchheim Institute of Pharmacology, Justus Liebig University, Giessen, 35392, Germany

    K. Beuerlein

Authors
  1. P. V. Zelenikhin
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  2. A. S. Gorbunova
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  3. K. Beuerlein
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  4. A. V. Makeeva
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  5. O. N. Ilinskaya
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Corresponding author

Correspondence to A. V. Makeeva.

Additional information

Original Russian Text © P.V. Zelenikhin, A.S. Gorbunova, K. Beuerlein, A.V. Makeeva, O.N. Ilinskaya, 2018, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2018, Vol. 73, No. 1, pp. 10–15.

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Zelenikhin, P.V., Gorbunova, A.S., Beuerlein, K. et al. Binase-Induced Changes of Tumor Cell Membranes. Moscow Univ. Biol.Sci. Bull. 73, 7–12 (2018). https://doi.org/10.3103/S0096392518010091

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  • DOI: https://doi.org/10.3103/S0096392518010091

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