Lysozyme activity of the salivary gland secretion of the medicinal leeches H. verbana, H. medicinalis, and H. orientalis

Abstract

Salivary gland secretions of three species of the medicinal leech differ in the level of their lysozyme, and peptidoglycan-lysing activity. Using a synthetic fluorogenic substrate, 4-methylumbelliferyltetra N-acetyl-β-chitotetraoxide, the glycosidase activity (as one of peptidoglycan-lysing activities) of salivary gland secretion of these species of the medicinal leech was quantitatively evaluated in comparison with egg lysozyme. It is suggested that lysozyme activity of the leech secretions is determined not only by 5 isoforms of destabilase-lysozyme, but by some other enzymes which can utilize these substrates including lysozymes other than i-type (invertebrate) lysozymes.

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Correspondence to I. P. Baskova.

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Original Russian Text © I.P. Baskova, O.V. Kharitonova, L.L. Zavalova, 2012, published in Biomeditsinskaya Khimiya.

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Baskova, I.P., Kharitonova, O.V. & Zavalova, L.L. Lysozyme activity of the salivary gland secretion of the medicinal leeches H. verbana, H. medicinalis, and H. orientalis . Biochem. Moscow Suppl. Ser. B 6, 138–143 (2012). https://doi.org/10.1134/S1990750812020035

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Keywords

  • medicinal leech salivary gland secretion
  • lysozyme activity
  • glycosidase activity
  • substrate 4-methylumbelliferyl-tetra N-acetyl-β-chitotetraoxide
  • egg lysozyme
  • destabilase-lysozyme