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Increased myeloperoxidase activity is a risk factor for ischemic heart disease in patients with diabetes mellitus

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Abstract

Using a previously developed spectrophotometric method (Bioorg. Khim. 2009, vol. 35, pp. 629–639) a significant increase of myeloperoxidase (MPO) activity (versus healthy control) was found in blood plasma of patients with type 2 diabetes mellitus (DM2) without cardiovascular complications, and also in patients with ischemic heart disease (IHD). The plasma MPO concentration measured by an enzyme-linked immunosorbent assay was significantly higher only in blood plasma of patients with DM2 and IHD. A significant positive correlation between blood MPO activity and blood MPO content was observed only in blood plasma samples from healthy donors. Increased MPO activity did not correlate with MPO concentration in blood plasma of patients with DM2 and DM2 with IHD. Taken together, these results indicate that studies on the MPO role in the development of pathological processes should include simultaneous determination of both the amount of enzyme and its peroxidase activity in blood of patients. The proposed approach gives comprehensive information about the relationship between MPO activity and MPO concentration in patient’s blood. Since the high concentration of MPO is a diagnostically important parameter for the prediction of development of endothelial dysfunction and cardiovascular diseases, the obtained results point to the contribution of MPO-dependent reactions in cardiovascular complications associated with diabetes mellitus. MPO activity may serve as an additional diagnostic criterion for determination of risk of IHD in DM patients.

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Abbreviations

DM2:

type 2 diabetes mellitus

ELISA:

enzyme-linked immunosorbent assay

fMLP:

N-formyl-Met-Leu-Phe

IHD:

ischemic heart disease

LDL:

low density lipoproteins

MPO:

myeloperoxidase

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Authors and Affiliations

  1. Department of Biophysics, Belarusian State University, ul. Nezavisimosty, 4, Minsk, 220030, Belarus

    I. V. Gorudko & S. N. Cherenkevich

  2. Institute for Experimental Medicine, North-West Branch of the Russian Academy of Medical Sciences, Saint-Petersburg, Russia

    V. A. Kostevich, A. V. Sokolov, E. T. Zakharova & V. B. Vasilyev

  3. Research Institute of Physico-Chemical Medicine, Moscow, Russia

    V. A. Kostevich, A. V. Sokolov & O. M. Panasenko

  4. Republican Science-Practical Center Cardiology, Minsk, Belarus

    I. V. Buko, E. E. Konstantinova, N. L. Tsapaeva & E. V. Mironova

Authors
  1. I. V. Gorudko
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  2. V. A. Kostevich
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  3. A. V. Sokolov
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  4. I. V. Buko
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  5. E. E. Konstantinova
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  6. N. L. Tsapaeva
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  7. E. V. Mironova
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  8. E. T. Zakharova
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  9. V. B. Vasilyev
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  10. S. N. Cherenkevich
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  11. O. M. Panasenko
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Corresponding author

Correspondence to I. V. Gorudko.

Additional information

Original Russian Text © I.V. Gorudko, V.A. Kostevich, A.V. Sokolov, I.V. Buko, E.E. Konstantinova, N.L. Tsapaeva, E.V. Mironova, E.T. Zakharova, V.B. Vasilyev, S.N. Cherenkevich, O.M. Panasenko, 2011, published in Biomeditsinskaya Khimiya.

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Gorudko, I.V., Kostevich, V.A., Sokolov, A.V. et al. Increased myeloperoxidase activity is a risk factor for ischemic heart disease in patients with diabetes mellitus. Biochem. Moscow Suppl. Ser. B 5, 307–312 (2011). https://doi.org/10.1134/S199075081103005X

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  • DOI: https://doi.org/10.1134/S199075081103005X

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