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Comparative proteomic analysis of cancerous and adjacent normal lung tissues

  • Nanobiotechnologies, Proteomics and Bioinformatics
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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

Lung cancer is the leading cause of cancer-related mortality in industrialized countries. Unfortunately, most lung cancers are found too late for a cure, therefore early detection and treatment is very important. We have applied proteomic analysis by using two-dimensional gel electrophoresis and peptide mass fingerprinting techniques for examination of cancerous and adjacent non-cancerous lung tissues from the same patient. The aim of the study was to find proteins, which could be used as biomarkers for diagnosis and monitoring of this disease. Indeed, we found differences in expression of several proteins, related to various cellular activities, such as, chaperoning (e,g. GRP96, GRP78, HSP27), metabolism and oxidation stress (e.g. L-fucose, GST), cytoskeleton (e.g., tubulin beta 2/3, beta actin), cell adhesion (e.g. annexin A5/3), binding proteins (e.g. 14-3-3 theta) and signal transduction. These changes may be important for progression of carcinogenesis; they may be used as the molecular-support for future diagnostic markers.

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References

  1. Anderson, L. and Seilhamet, J., Electrophoresis, 1997, vol. 18, pp. 533–537.

    Article  CAS  Google Scholar 

  2. Wilkins, M.R., Sanchez, J.C., Williams, K.L., and Hochstrasser, D.F., Electrophoresis, 1996, vol. 17, pp. 830–838.

    Article  CAS  Google Scholar 

  3. Chang, J.W., Jeon, H.B., Lee, J.H., Yoo, J.S., Chun, J.S., Kim, J.H., and Yoo, Y.J., Biochem. Biophys. Res. Commun., 2001, vol. 289, pp. 507–512.

    Article  CAS  Google Scholar 

  4. Kanamoto, T., Hellman, U., Heldin, C.H., and Souchelnytskyi, S., EMBO. J., 2002, vol. 21, pp. 1219–1230.

    Article  CAS  Google Scholar 

  5. Nishio, K., Nakamua, T., Koh, Y., Kanzawa, F., Tamura, T., and Saijo, N., Cancer, 2001, vol. 97, pp. 1494–1499.

    Article  Google Scholar 

  6. Predic, J., Soskic, V., Bradley, D., and Godovac-Zimmermann, J., Biochemistry, 2002, vol. 41, pp. 1070–1078.

    Article  CAS  Google Scholar 

  7. Griese, M., von Bredwo, C., and Birrer, P. Electrophoresis, 2001, vol. 22, pp. 165–171.

    Article  CAS  Google Scholar 

  8. Chen, G., Gharib, T.G., Huang, C.C., Taylor, J.M., Misek, D.E., Kardia, S.L., Giordano, T.J., Iannettoni, M.D., Orringer, M.B., Hanash, S.M., and Beer, D.G., Mol. Cell Proteomics, 2002, vol. 1, pp. 304–313.

    Article  CAS  Google Scholar 

  9. Brichory, F., Beer, D., Le Naour, F., Giordana, T., and Hanash, S., Cancer Res., 2001, vol. 61, pp. 7908–7912.

    CAS  Google Scholar 

  10. Hanash, S., Brichory, F., and Beer, D., Dis. Markers, 2001, vol. 17, pp. 295–300.

    CAS  Google Scholar 

  11. Oh, J.M., Brichory, F., and Puravs, E., Proteomics, 2001, vol. 1, pp. 1303–1319.

    Article  CAS  Google Scholar 

  12. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M., Anal. Chem., 1996, vol. 68, pp. 850–858.

    Article  CAS  Google Scholar 

  13. Huang, H.L., Stasyk, T., Morandell, S., Dieplinger, H., Falkensammer, G., Griesmacher, A., Mogg, M., Schreiber, M., Feuerstein, I., Huck, C.W., Stecher, G., and Bonn, G.K., Electrophoresis, 2006, vol. 27, pp. 1641–1650.

    Article  CAS  Google Scholar 

  14. Cui, L., Zhuchu, C., Zhiqiang, X., Xiaoying, W., Xianquan, Z., Xiaopeng, Z., Maoyu. L., JianLing, L., Xueping, F., Songping, L., Ping, C., and Jing-yun, X., Biochem. Biophys. Res. Commun., 2003, vol. 309, pp. 253–260.

    Article  Google Scholar 

  15. Shiu, R.P. and Pastan, I.H., Biochem. Biophys. Acta., 1979, vol. 576, pp. 141–150.

    CAS  Google Scholar 

  16. Jamora, C., Dennert, G., and Lee, A.S., Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 7690–7694.

    Article  CAS  Google Scholar 

  17. Koong, A.C., Chen, E.Y., Lee, A.S., Brown, J.M., and Giaccia, A.J., Int. J. Radiat. Oncol. Biol. Phys., 1994, vol. 28, pp. 661–666.

    CAS  Google Scholar 

  18. Li, L.J., Li, X., Ferrario, A., Rucker, N., Liu, E.S., Wong, S., Gomer, C.J., and Lee, A.S., J. Cell Physiol., 1992, vol. 153, pp. 575–582.

    Article  CAS  Google Scholar 

  19. Melnick, J., Dull, J.L., and Argon, Y., Nature, 1994, vol. 370, pp. 373–375.

    Article  CAS  Google Scholar 

  20. Little, E. and Lee, A.S., J. Biol. Chem., 1995, vol. 270, pp. 9526–9534.

    Article  CAS  Google Scholar 

  21. Strivastava, P.K. and Old, L., Immunol. Today, 1988, vol. 9, pp. 78–83.

    Article  Google Scholar 

  22. Udono, H., Levey, D.L., and Srivastava, P.K., Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 3077–3081.

    Article  CAS  Google Scholar 

  23. Morimoto, R.I., Science, 1993, vol. 259, pp. 1409–1410.

    Article  CAS  Google Scholar 

  24. Isomoto, H., Oka, M., Yano, Y., Kanazawa, Y., Soda, H., Terada, R., Yasuake, T., Nakayama, T., Shikuwa, S., Takeshima, F., Udono, H., Murata, I., Ohtsuka, K., and Kohno, S., Cancer Lett., 2003, vol. 198, pp. 219–228.

    Article  CAS  Google Scholar 

  25. Tanner, F., Sutterlin, M.W., Kapp, M., Rieger, L., Kristen, P., Dietl, J., Gassel, A.M., and Müller, T., Anticancer Res., 2003, vol. 23, pp. 1057–1062.

    Google Scholar 

  26. Wozniak, A., Drewa, T., Rozwodowska, M., Drewa, G., Lambrecht, W., and Wisniewska, I., Neoplasma, 2002, vol. 49, pp. 10–15.

    CAS  Google Scholar 

  27. Ishihara, H. and Heath, E.C., (1968) J. Biol. Chem., vol. 243, pp. 1110–1115.

    CAS  Google Scholar 

  28. Noda, K., Miyoshi, E., Gu, J., Gao, C.X., Nakahara, S., Kitada, T., Honke, K., Suzuk, K., Yoshihara, H., Yoshikawa, K., Kawano, K., Tonetti, M., Kasahara, A., Hori, M., Hayashi, N., and Taniguchi, N., Cancer Res., 2003, vol. 63, pp. 6282–6289.

    CAS  Google Scholar 

  29. Ogawa, J., Inoue, H., and Koide, S., Cancer (Phila.), 2003, vol. 79, pp. 1678–1685.

    Article  Google Scholar 

  30. Achiwa, H., Sato, S., Shimizu, S., Maeda, H., Niimi, T., Takahashi, T., Ueda, R., and Mitsudomi, T., Cancer Lett., 2003, vol. 201, pp. 211–216.

    Article  CAS  Google Scholar 

  31. Raynal, P. and Pollard, H.B., Biochim. Biophys. Acta., 1994, vol. 1197, pp. 63–93.

    CAS  Google Scholar 

  32. Rhee, H.J., Kim, G.Y., Huh, J.W., Kim, S.W., and Na, D.S., Eur. J. Biochem., 2000, vol. 267, pp. 3220–3225.

    Article  CAS  Google Scholar 

  33. Moncrief, N.D., Kretsinger, R.H., and Goodman, M., J. Mol. Evol., 1990, vol. 30, pp. 522–562.

    Article  CAS  Google Scholar 

  34. Hermani, A., Hess, J., De Servi, B., Medunjanin, S., Groholz, R., Trojan, L., and Angel, P., Clin. Cancer Res., 2005, vol. 11, pp. 5146–5152.

    Article  CAS  Google Scholar 

  35. Tzuvuibm, G., Gupta, V.S., Kaplun, L., and Balan, V., Seminars in Cancer Biology, 2006, vol. 16, pp. 203–213.

    Article  Google Scholar 

  36. Hermeking, H., Nat. Rev. Cancer, 2003, vol. 3, pp. 931–943.

    Article  CAS  Google Scholar 

  37. Wilker, E. and Yaffe, M.B., Mol. Cell Cardiol., 2004, vol. 37, pp. 633–642.

    Article  CAS  Google Scholar 

  38. Burbelo, P., Wellstein, A., and Pestell, R., Breast Cancer Res. Treat., 2004, vol. 84, no. 1, pp. 43–48.

    Article  CAS  Google Scholar 

  39. Heike, R., Petra, Z., Roman, H., and Jörgen, B., Proteomics, 2006, vol. 6, pp. 3127–3137.

    Article  Google Scholar 

  40. Schultz, J., Milpetz, F., Bork, P., and Ponting, C.P., Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 5857–5864.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, SongDo Techno Park, 7-50 Songdo-Dong, Yeonsu Gu, Incheon, 406-840, South Korea

    KiBeom Lee

  2. Research Institute, AdipoGen, Room #401, Venture Building B, SongDo TechnoPark, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon, 406-840, South Korea

    Kyung Bae Pi

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  1. KiBeom Lee
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Correspondence to KiBeom Lee.

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Original Russian Text © KiBeom Lee, Kyung Bae Pi, 2010, published in Biomeditsinskaya Khimiya.

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Lee, K., Pi, K.B. Comparative proteomic analysis of cancerous and adjacent normal lung tissues. Biochem. Moscow Suppl. Ser. B 4, 123–129 (2010). https://doi.org/10.1134/S1990750810020022

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  • DOI: https://doi.org/10.1134/S1990750810020022

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