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Production of reactive oxygen species by monocyte-derived macrophages from blood of healthy donors and patients with ischemic heart disease

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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

Production of reactive oxygen species (ROS) by macrophages derived from blood monocytes of healthy donors (MPN) and patients with ischemic heart disease (IHD) (MPIHD) before, during, and after their incubation with low-density lipoprotein (LDL) isolated from blood plasma of healthy donors (LDLN) and patients with a high cholesterol level (LDLH) was investigated by the method of luminol-dependent (spontaneous) and stimulated chemiluminescence (CL) using opsonized zymosan (OZ) or phorbol-12-myristate-13-acetate (PMA) as the CL stimulators. It was shown that proper, luminol-dependent, and zymosan-or PMA-stimulated chemiluminescence of MPIHD was 1.4-, 1.8-, 2.7-, and 1.6-fold higher than the same types of chemiluminescence of MPN, respectively, (p<0.05–0.01). Although the effect of OZ on MPN and MPIHD was more potent than that of PMA (by 4.3- and 3.2-fold, respectively), but it appeared in 2.5–3.0 times slower than that of PMA. LDLN and LDLH incubated with MPN for the first 15 and 60 min caused the 1.4- and 2.5-increase of the luminol-dependent CL of MPN; the same treatment of MPIHD did not influence ROS production by these cells. Repeated increase in the OZ-stimulated CL of MPN was also observed after preincubation for 15–180 min with LDLN and LDLH followed by LDL removal, subsequent MPN washing and addition of Hanks solution and OZ; the repeated increase in OZ-stimulated CL of MPN was only observed after incubation with LDLH than with LDLN. No increase of CL was observed in experiments with MPIHD. Thus, more intensive chemiluminescence of macrophages obtained from blood of patients with IHD suggests their in vivo stimulation. LDLN and LDLH may cause both primary and secondary (after preincubation) stimulating effect on CL of MPN but not of MPIHD. Thus, the analysis of macrophage chemiluminescence is a sensitive test for evaluation the degree of macrophage stimulation; it may be effectively used for monitoring of effectiveness of medical treatment of patients.

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Abbreviations

CL:

chemiluminescence

IHD:

ischemic heart disease

LDL:

low density lipoproteins

LDLH :

LDL from the blood plasma of hypercholesterolemic patients

LDLN :

LDL from blood plasma of healthy donors

MP:

macrophages obtained from human blood

MPIHD :

macrophages from IHD patients

MPN :

macrophages from healthy donors

OZ:

opsonized zymosan

PMA:

phorbol-12-myristate-13-acetate

ROS:

reactive oxygen species

TBARS:

thiobarbituric acid-reactive substances

References

  1. Parthasarathy, S., Steinberg, D., and Witztum, J.L., Annu. Rev. Med., 1992, vol. 43, pp. 219–225.

    Article  CAS  Google Scholar 

  2. Takahashi, K., Takeya, M., and Sakashita, N., Annu. Rev. Med., 2002, vol. 35, no. 4, pp. 179–203.

    CAS  Google Scholar 

  3. Bilenko, M.V., Khil’chenko, A.V., and Shmit’ko, N.A., Byull. Eksp. Biol. Med., 2003, vol. 135, no. 4, pp. 410–413.

    Article  Google Scholar 

  4. Klebanov, G.I. and Vladimirov, Yu.A., Usp. Sovrem. Biol., 1999, vol. 119, pp. 461–474.

    Google Scholar 

  5. Bilenko, M.V., Ischemia and Reperfusion of Various Organs: Injury Mechanisms, Methods of Prevention and Treatment, Boriotti, S. and Dennis, D., Eds., Huntington: Nova Science Publishers, Inc., 2001.

    Google Scholar 

  6. Bilenko, M.V., Khilchenko, A.V., and Nikitina, N.A., J. Mol. Cell. Cardiol., 2004, vol. 37, no. 1, pp. 242–243.

    Google Scholar 

  7. Bilenko, M.V., Khilchenko, A.V., Nikitina, N.A., and Aksenov, D.V., Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2008, vol. 2, pp. 395–405.

    Article  Google Scholar 

  8. Klebanov, G.I., Kreinina, M.V., Pozin, V.M., Skuratovskaya, S.G., Pocheptsova, G.A., and Vladimirov, Yu.A., Byull. Eksp. Biol. Med., 1988, vol. 106, no. 9, pp. 297–299.

    CAS  Google Scholar 

  9. Filipov, A.E., Chechetkin, A.V., Danil’chenko, V.A., Danil’chenko, V.V., Kas’yanov, A.N., and Vaschenko, V.I., Terra Medica Nova, 2004, vol. 1, no. 3, pp. 33–37.

    Google Scholar 

  10. Uchiyama, M. and Mihara, M., Anal. Biochem., 1978, vol. 86, pp. 271–278.

    Article  CAS  Google Scholar 

  11. Morel, D.W., Hessler, J.R., and Chisolm, G.M., J. Lipid Res., 1983, vol. 24, pp. 1070–1076.

    CAS  Google Scholar 

  12. Nanda, A. and Grinstein, S., Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 10816–10820.

    Article  CAS  Google Scholar 

  13. Tohyama, Y.I. and Yamamura, H., IUBMB Life, 2006, vol. 58, pp. 304–308.

    Article  CAS  Google Scholar 

  14. Witztum, J.L. and Steinberg, D., J. Clin. Invest., 1991, vol. 88, pp. 1785–1792.

    Article  CAS  Google Scholar 

  15. Lavy, A., Brook, G.J., Dankner, G., Ben Amotz, A., and Aviram, M., Metabolism, 1991, vol. 40, pp. 794–799.

    Article  CAS  Google Scholar 

  16. Handberg, A., Levin, K., Hojlund, K., Beck-Nielsen, H., Circulation, 2006, vol. 114, pp. 1169–1176.

    Article  CAS  Google Scholar 

  17. Kopprasch, S., Pietzsch, J., and Graessler, J., Luminescence, 2003, vol. 8, pp. 268–273.

    Article  Google Scholar 

  18. Voevoda, M.V., Ragino, Yu.I., Semaeva, E.V., Kashtanova, E.V., Ivanova, M.V., Chernyavskii, A.M., and Nikitin, Yu. P., Byull. SB RAMS, 2003, vol. 109, no. 3, pp. 47–50.

    Google Scholar 

  19. Calvo, D., Gómez-Coronado, D., Suárez, Y., Lasunción, M.A., and Vega, M.A., J. Lipid Res., 1998, vol. 39, 777–788.

    CAS  Google Scholar 

  20. Hultén, L.M., Ullström, C., Krettek, A., Van Reyk, D., Marklund, S.L., Dahlgren, C., and Wiklund, O., Lipids Health Dis., 2005, vol. 4, no. 1, pp. 6–17.

    Article  Google Scholar 

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Authors and Affiliations

  1. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul. 10, Moscow, 119121, Russia

    M. V. Bilenko, S. A. Pavlova, Nguyen Thi Thu Thuy & Tran Thi Hai Yen

  2. Faculty of Basic Medicine, Moscow State University, Moscow, 119992, Russia

    Yu. A. Vladimirov

Authors
  1. M. V. Bilenko
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  2. Yu. A. Vladimirov
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  3. S. A. Pavlova
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  4. Nguyen Thi Thu Thuy
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  5. Tran Thi Hai Yen
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Correspondence to M. V. Bilenko.

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Original Russian Text © M.V. Bilenko, Yu.A. Vladimirov, S.A. Pavlova, Nguyen Thi Thu Thuy, Tran Thi Hai Yen, 2009, published in Biomeditsinskaya Khimiya.

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Bilenko, M.V., Vladimirov, Y.A., Pavlova, S.A. et al. Production of reactive oxygen species by monocyte-derived macrophages from blood of healthy donors and patients with ischemic heart disease. Biochem. Moscow Suppl. Ser. B 3, 64–70 (2009). https://doi.org/10.1134/S1990750809010090

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  • DOI: https://doi.org/10.1134/S1990750809010090

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