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Study of the interaction of trypsin inhibitor from the sea anemone Radianthus macrodactylus with proteases

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Abstract

The interaction of the inhibitor VJ (InhVJ), isolated from sea anemone R. macrodactylus, with different proteases was investigated using the method of biosensor analysis. The following enzymes were tested: serine proteases (trypsin, α-chymotrypsin, plasmin, thrombin, kallikrein), cysteina protease (papain) and aspartic protease (pepsin). In the rage of the concentrations studied (10–400 nM) inhibitor VJ interacted only with trypsin and α-chymotrypsin. The intermolecular complexes formation between inhibitor VJ and each of these enzymes was characterized by the following kinetic and thermodynamics parameters: KD = 7.38 × 10−8 M and 9.93 × 10−7 M for pairs InhVJ/trypsin and InhVJ/α-chymotrypsin, respectively.

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References

  1. Birk, Y., Int. J. Pept. Protein Res., 1985, vol. 25, pp. 113–131.

    Article  PubMed  CAS  Google Scholar 

  2. Christensen, S. and Sottrup-Jensen, L., Biochem. J., 1994, vol. 303, pp. 383–390.

    PubMed  CAS  Google Scholar 

  3. Mosolov, V.V. and Valueva, T.A., Prikl. Biokhim. Mikrobiol., 2005, vol. 41, pp. 261–282.

    PubMed  CAS  Google Scholar 

  4. Vadgama, P. and Crump, P.W., Analyst, 1992, vol. 117, pp. 1657–1670.

    Article  CAS  Google Scholar 

  5. O’shannesy, D.J., Curr. Opin. Biothechnol., 1994, vol. 5, pp. 65–71.

    Article  Google Scholar 

  6. Szabo, A., Stolz, L., and Granzow, R., Curr. Opin. Struct. Biol., 1995, vol. 5, pp. 699–705.

    Article  PubMed  CAS  Google Scholar 

  7. Brigham-Burke, M., Edwards, J.R., and O’shannesy, D.J., Anal. Biochem., 1992, vol. 205, pp. 125–131.

    Article  PubMed  CAS  Google Scholar 

  8. O’shannesy, D.J., Brigham-Burke, M., and Peck, K., Anal. Biochem., 1992, vol. 205, pp. 125–131.

    Article  Google Scholar 

  9. Deinum, J., Gustavsson, L., Gyzander, E., Kullman-Magnusson, M., Edstrom, A., and Karlsson, R., Anal. Biochem., 2002, vol. 300, pp. 152–162.

    Article  PubMed  CAS  Google Scholar 

  10. Delfin, J., Martinez, I., Antuch, W., Morera, V., Gonzalez, Y., Rodriguez, R., Marquez, M., Saroyan, A., Larionova, N., Diaz, J., Padron, G., and Chavez, M., Toxicon, 1996, vol. 34, pp. 1367–1376.

    Article  PubMed  CAS  Google Scholar 

  11. Minagawa, S., Ishida, M., Shimakura, K., Nagashima, Y., and Shiomi, K., Comp. Biochem. Physiol. (B) Biochem. Mol. Biol., 1997, vol. 118(2), pp. 381–386.

    Article  CAS  Google Scholar 

  12. Mebs, D., Liebrich, M., Reul, A., and Samejima, Y., Toxicon, 1983, vol. 21, pp. 257–264.

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, pr. 100 let Vladivostoku 159, Vladivostok, 690022, Russia

    I. N. Sokotun, A. V. Leychenko, M. M. Monastyrnaya, E. P. Kozlovskaya & A. A. Molnar

  2. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul., 10, Moscow, 119121, Russia

    O. V. Gnedenko & A. S. Ivanov

Authors
  1. I. N. Sokotun
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  2. O. V. Gnedenko
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  3. A. V. Leychenko
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  4. M. M. Monastyrnaya
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  5. E. P. Kozlovskaya
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  6. A. A. Molnar
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  7. A. S. Ivanov
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Corresponding author

Correspondence to A. S. Ivanov.

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Original Russian Text © I.N. Sokotun, O.V. Gnedenko, A.V. Leychenko, M.M. Monastyrnaya, E.P. Kozlovskaya, A.A. Molnar, A.S. Ivanov, 2007, published in Biomeditsinskaya Khimiya.

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Sokotun, I.N., Gnedenko, O.V., Leychenko, A.V. et al. Study of the interaction of trypsin inhibitor from the sea anemone Radianthus macrodactylus with proteases. Biochem. Moscow Suppl. Ser. B 1, 139–142 (2007). https://doi.org/10.1134/S1990750807020059

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  • DOI: https://doi.org/10.1134/S1990750807020059

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