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Structural Characteristic of Nucleolus and Heterochromatin Aggregates of Rat Brain Tanycytes

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract—

This work was aimed at studying the structural organization of nucleolus and constitutive heterochromatin in different types of tanycytes during postnatal development and aging of rats. The distribution of nucleolus argentophilic proteins (nucleolin and nucleophosmin) and heterochromatin aggregates in tanycytes at various stages of postnatal development have been described for the first time using immunohistochemical methods and confocal laser microscopy. The heterogeneity of the size and number of nucleoli was demonstrated both in different tanycytes subpopulations and at different ages of an animal. This may indicate different levels of the tanycyte synthetic activity and the ability to proliferate during early postnatal development and aging. During aging, the distribution of heterochromatin aggregates varies among tanycyte subpopulations: α-tanycytes undergo intense heterochromatization, while β-tanycytes are characterized by a stable organization of the studied compartments of the cell nucleus. The data obtained significantly supplement the modern understanding of organization of the structure of the cell nucleus of tanycytes during normal development and aging. This can subsequently serve as a basis for establishing the role of these subnuclear structures in pathological processes.

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ACKNOWLEDGMENTS

The work was supported by the State Assignment for the Institute of Experimental Medicine. The images were obtained at the Human Microbiome Center for Collective Use of Scientific Equipment of the Institute of Experimental Medicine.

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Authors and Affiliations

  1. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    D. A. Sufieva, I. M. Pleshakova & D. E. Korzhevskii

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  1. D. A. Sufieva
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  2. I. M. Pleshakova
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  3. D. E. Korzhevskii
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Correspondence to D. A. Sufieva.

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The authors declare that they have no conflict of interest.

All procedures were performed in accordance with the European Communities Council Directive (November 24, 1986; 86/609/EEC) and the Declaration on humane treatment of animals. The protocol of experiments was approved by the Commission on Bioethics of the Institute of Experimental Medicine.

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Translated by E. Makeeva

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Sufieva, D.A., Pleshakova, I.M. & Korzhevskii, D.E. Structural Characteristic of Nucleolus and Heterochromatin Aggregates of Rat Brain Tanycytes. Biochem. Moscow Suppl. Ser. A 15, 319–328 (2021). https://doi.org/10.1134/S199074782105007X

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  • DOI: https://doi.org/10.1134/S199074782105007X

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