Abstract
It is shown that upon oxidation of succinate in the presence of rotenone and antioxidant Trolox (or pyruvate) in liver mitochondria of mature rats (9–12-month old) the respiration stimulated by palmitate is suppressed by ADP (the substrate of ADP/ATP-antiporter) and aspartate (the substrate of aspartate/glutamate antiporter). However, it was found that in the presence of the oxidative agent tert-butylhydroperoxide neither ADP nor aspartate is effective even at their joint action. In the presence of ADP and aspartate, uncoupling activity of palmitate is minimal, since the lipid peroxidation is inhibited by Trolox or pyruvate, and rises as the accumulation rate of conjugated dienes increases, reaching the maximal value at the oxidative stress caused by tert-butylhydroperoxide. In liver mitochondria of senile rats (22–26-month old) at high intensity of lipid peroxidation, ADP and aspartate do not affect the uncoupling activity of palmitate (Samartsev and Kozhina, 2008, Biochemistry (Mosc.), vol. 73, no. 7, pp. 783–790). Comparative studies have shown that in liver mitochondria of mature and senile rats at the similar accumulation rate of the conjugated dienes in the presence of ADP and aspartate, the uncoupling activity of palmitate reaches the same level relative to the maximal activity. We conclude that an enhancement of free radical reactions and lipid peroxidation in liver mitochondria can result in an increase of protonophore uncoupling activity of fatty acids with the involvement of ADP/ATP- and aspartate/glutamate antiporters due to the suppression of the ability of physiological substrates of these carriers of ADP and aspartate to inhibit the uncoupling process.
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Original Russian Text © O.V. Kozhina, V.N. Samartsev, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 2, pp. 184–188.
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Kozhina, O.V., Samartsev, V.N. Uncoupling activity of fatty acids in liver mitochondria in the presence of substrates of ADP/ATP- and aspartate/glutamate antiporters is increased under oxidative stress. Biochem. Moscow Suppl. Ser. A 4, 196–199 (2010). https://doi.org/10.1134/S1990747810020108
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DOI: https://doi.org/10.1134/S1990747810020108