Abstract
Electrokinetic measurements are carried out in suspensions of liposomes made from mixtures of charged (cardiolipin, CL) and neutral (phosphatidylcholine, PC) lipids in the presence of lysine and lysine-based polypeptides. Neither monolysine nor polylysines adsorbed on neutral (PC) membranes. In the case of negatively charged membranes (CL/PC) all polypeptides showed a sharp dependence of liposome electrophoretic mobility on the amount of polymer added to the cell. In suspension of cardiolipin liposomes the position of zero charge point coincided for all high-molecular polylysines; thus, pentalysine neutralizes the membrane surface, whereas polycations with a higher polymerization degree change a sign of the surface charge. Electrophoretic mobility of liposomes in plateau range depended on the molecular weight of polylysines and composition of liposomes; for large macromolecules the absolute value came close to its value for the initial liposomes. Adsorption of polycations on planar bilayer lipid membranes (BLM) resulted in alteration of the boundary potential measured by the method of intramembranous field compensation (IFC). The electrokinetic measurements and IFC method gave close results in the case of lysine monomers; their surface concentration could be fitted by an isotherm of the molecule distribution between the membrane surface and solution. Considerable differences of the surface and boundary potentials found in the case of pentalysine, correspond to changes in the dipole component of boundary potential induced by the adsorbed molecules. Using the IFC method, the kinetics of the adsorption process before saturation was studied. The adsorption of polylysines was markedly slower (more than hour) than that of pentalysine (tens of min) or monolysine (minutes). Washout experiments showed that adsorption of penta-and monolysine on planar BLM was reversible, while that of high-molecular polylysines was practically irreversible.
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Lv, H., Zhang, S., Wang, B., Cui, S., and Yan, J., Toxicity of Cationic Lipids and Cationic Polymers in Gene Delivery, J. Control Release, 2006, vol. 114, no. 1, pp. 100–109.
Atanasov, V., Knorr, N., Duran, R.S., Ingebrandt, S., Offenhäusser, A., Knoll, W., and Koeper, I., Membrane on a Chip: A Functional Tethered Lipid Bilayer Membrane on Silicon Oxide Surfaces, Biophys. J., 2005, vol. 89, no. 3, pp. 1780–1788.
Zintchenko, A. and Konak, C., Interaction of DNA/Polycation Complexes with Phospholipids: Stabilising Strategy for Gene Delivery, Macromol. Biosci., 2005, vol. 5, no. 12, pp. 1169–1174.
Ravi, K.M., Hellermann, G., Lockey, R.F., and Mohapatra, S.S., Nanoparticle-Mediated Gene Delivery: State of the Art, Expert. Opin. Biol. Ther., 2004, vol. 4, no. 8, pp. 1213–1224.
Demina, T., Melik-Nubarov, N., Shtil, A., Frey, H., Pohl, P., and Pohl, E.E., Relationship between Structure of Amphiphilic Copolymers and Their Ability to Cause Chemosensitization of Multi-Drug Resistant Tumour Cells, Biophys. J., 2005, vol. 88, no. 1, pp. 595A–595A.
Melik-Nubarov, N.S., Dorodnykh, T.Y., Batrakova, E.V., Kozlov, M.Y., Suzdal’tseva, Y.G., Kabanov, A.V., Alakhov, V.Y., and Arzhakov, S.A., Synthesis and Biological Activity of the Functional Block-Copolymers Based on Pluronic P85-Doxorubicin Conjugates, Vysokomolekulyarnye Soedineniya, Seriya A i Seriya B (Rus.), 1999, vol. 41, no. 5, pp. 768–775.
Menger, F.M., Seredyuk, V.A., Kitaeva, M.V., Yaroslavov, A.A., and Melik-Nubarov, N.S., Migration of Poly-L-Lysine through a Lipid Bilayer, J. Amer. Chem. Soc., 2003, vol. 125, no. 10, pp. 2846–2847.
Pashkovskaya, A.A., Lukashev, E.P., Antonov, P.E., Finogenova, O.A., Ermakov, Y.A., Melik-Nubarov, N.S., and Antonenko, Y.N., Grafting of Polylysine with Polyethylenoxide Prevents Demixing of O-Pyromellitylgramicidin in Lipid Membranes, Biochim. Biophys. Acta, 2006, vol. 1758, pp. 1685–1695.
Mosior, M. and McLaughlin, S., Peptides That Mimic the Pseudosubstrate Region of Protein Kinase C Bind to Acidic Lipids in Membranes, Biophys. J., 1991, vol. 60, no. 1, pp. 149–159.
Denisov, G., Wanaski, S., Luan, P., Glaser, M., and McLaughlin, S., Binding of Basic Peptides to Membranes Produces Lateral Domains Enriched in the Acidic Lipids Phosphatidylserine and Phosphatidylinositol 4,5-Bisphosphate: An Electrostatic Model and Experimental Results, Biophys. J., 1998, vol. 74, no. 2, pp. 731–744.
Murray, D., Arbuzova, A., Hangyas-Mihalyne, G., Gambhir, A., Ben Tal, N., Honig, B., and McLaughlin, S., Electrostatic Properties of Membranes Containing Acidic Lipids and Adsorbed Basic Peptides: Theory and Experiment, Biophys. J., 1999, vol. 77, no. 6, pp. 3176–3188.
Ermakov, Yu.A., Fevraleva, I.S., and Ataullakhanov, R. I., The Influence of Polycations on the Boundary Potentials of BLM, Biologicheskie Membrany (Rus.), 1985, vol. 2, no. 11, pp. 1094–1100.
Ermakov, Yu.A., Bioelectrochemistry of Lipid Membranes, Rossiiskii Khimicheskii Zhurnal (Rus.), 2005, vol. 49, no. 5, pp. 114–121.
Yaroslavov, A.A., Sitnikova, T.A., Rakhnyanskaya, A.A., Ermakov, Y.A., Burova, T.V., Grinberg, V.Y., and Menger, F.M., Contrasting Behavior of Zwitterionic and Cationic Polymers Bound to Anionic Liposomes, Langmuir, 2007, vol. 23, no. 14, pp. 7539–7544.
Yaroslavov, A.A., Efimova, A.A., Lobyshev, V.I, and Ermakov, Yu.A., The Reversibility of Structural Changes in the Lipid Membranes Induced by Adsorption of Polycation, Biologicheskie Membrany (Rus.), 1996, vol. 13, no. 6, pp. 628–633.
Ermakov, Y.A., Averbakh, A.Z., Yusipovich, A.I., and Sukharev, S., Dipole Potentials Indicate Restructuring of the Membrane Interface Induced by Gadolinium and Beryllium Ions, Biophys. J., 2001, vol. 80, no. 4, pp. 1851–1862.
Ermakov, Yu.A., Equilibrium of Ions at the Lipid Membranes—The Empirical Analysis of the Simplest Model, Kolloidnyi Zhurnal (Rus.), 2000, vol. 6, no. 4, pp. 437–449.
Ermakov, Yu.A. and Sokolov, V.S., Boundary Potentials of Bilayer Lipid Membranes: Methods and Interpretations, Planar Lipid Bilayers (BLMs) and Their Applications, Tien, H.T. and Ottova, A., Eds., Elsevier, 2003, pp. 109–141.
Ermakov, Yu.A., Interaction of Polyelectrolytes with Bilayer Lipid Membranes, Elektrokhimiya Polimerov (Electrochemistry of Polymers), Moscow, Nauka, 1990, pp. 93–101.
Ermakov, Yu. A., The Determination of Binding Site Density and Association Constants for Monovalent Cation Adsorption onto Liposomes Made from Mixtures of Zwitterionic and Charged Lipids, Biochim. Biophys. Acta., 1990, vol. 1023, no. 1, pp. 91–97.
Hunter, R.J., Zeta Potential in Colloid Science. Principles and Applications, Colloid Science, Ottewill, R.H. and Rowell, R.L., Eds., London: Academic Press, 1981.
Cevc, G. and Marsh, D., Phospholipid Bilayers: Physical Principles and Models, Cell Biology: A Series of Monographs, Bittar, E.E., Ed., New York: Willey-Interscience Publication, 1987, vol. 5.
Ermakov, Y.A., Makhmudova, S.S., and Averbakh, A.Z., Two Components of Boundary Potentials at the Lipid Membrane Surface: Electrokinetic and Complementary Methods Studies, Colloids and Surfaces, A-Physicochemical and Engineering Aspects, 1998, vol. 140, nos. 1–3, pp. 13–22.
Ermakov, Y.A., Averbakh, A.Z., Arbuzova, A.B., and Sukharev, S.I., Lipid and Cell Membranes in the Presence of Gadolinium and Other Ions with High Affinity to Lipids. 2. The Dipole Components of a Boundary Potential of Membranes with Varied Surface Charge, Biologicheskie Membrany (Rus.), 1998, vol. 15, no. 3, pp. 330–341.
Ermakov, Yu.A., Averbakh, A.Z., and Sukharev, S.I., Lipid and Cellular Membranes in the Presence of Gadolinium and Other Ions with High Affinity to Lipids. 1. Dipole and Diffuse Components of the Boundary Potential, Biologicheskie Membrany (Rus.), 1997, vol. 14, no. 4, pp. 434–445.
Choi, E.J. and Dimitriadis, E.K., Cytochrome C Adsorption to Supported Anionic Lipid Bilayers Studied via Atomic Force Microscopy, Biophys. J., 2004, vol. 87, pp. 3234–3241.
Schwieger, C. and Blume, A., Interaction of Poly (L-Lysines) with Negatively Charged Membranes: An FT-IR and DSC Study, Eur. Biophys. J., 2007, vol. 36, nos. 4–5, pp. 437–450.
Yaroslavov, A.A., Koulkov, V.Ye., Polynsky, A.S., Baibakov, B.A., and Kabanov, V.A., A Polycation Causes Migration of Negatively Charged Phospholipids from the Inner to Outer Leaflet of the Liposomal Membrane, FEBS Lett., 1994, vol. 340, nos.1–2, pp. 121–123.
Yaroslavov, A.A., Kuchenkova, O.Y., Okuneva, I.B., Melik-Nubarov, N.S., Kozlova, N.O., Lobyshev, V.I., Menger, F.M., and Kabanov, V.A., Effect of Polylysine on Transformations and Permeability of Negative Vesicular Membranes, Biochim. Biophys. Acta, 2003, vol. 1611, nos. 1–2, pp. 44–54.
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Original Russian Text © O.A. Finogenova, D.V. Filinsky, Yu.A. Ermakov, 2008, published in Biologicheskie Membrany, 2008, Vol. 25, No. 3, pp. 230–238.
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Finogenova, O.A., Filinsky, D.V. & Ermakov, Y.A. Electrostatic effects upon adsorption and desorption of polylysines on the surface of lipid membranes of different composition. Biochem. Moscow Suppl. Ser. A 2, 181–188 (2008). https://doi.org/10.1134/S1990747808020128
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DOI: https://doi.org/10.1134/S1990747808020128